Daphnia Magna Experiment

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Introduction
The use of model organisms in pre clinical trial is relied upon for the collection of safety and efficacy data prior to first in human clinical studies. The crustacean Daphnia magna is one model organism, whose heart rate is reported to react similarly to the human heart rate, when exposed to identical compounds (3).
In human studies, caffeine and nicotine have been reported to increase the heart rate of human beings (2).

The purpose of this study is to determine the effect of varying concentration of alcohol, caffeine and nicotine on the heart rate of a daphnia magna and confirm any similarities between the affect of the chemical compound on the heart rate of daphnia magna and human beings.

Material and Method
The crustacean Daphnia magna, maintained in water at room temperature, was obtained. The chemical compound used are five (5) fold dilution of one (1) molar aqueous nicotine, five (5) fold dilution of one (1) molar aqueous caffeine, 10 fold dilution of one (1) molar aqueous nicotine, five (5) fold dilution of one (1) molar aqueous caffeine, water and 100% alcohol. …show more content…

The heartbeat per minute was calculated after exposure of Daphnia magna to one of the five chemical compounds listed above.
A total of twelve Daphnia magna were used for this experiment; two Daphnia magna were exposed simultaneously to each of the five chemical

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