Wait a second!
More handpicked essays just for you.
More handpicked essays just for you.
Dna replication biochemistry
Dna replication biochemistry
The process of transcription and translation in dna replication
Don’t take our word for it - see why 10 million students trust us with their essay needs.
Recommended: Dna replication biochemistry
The Process of Replication in a DNA Molecule
Replication is the process of when a double-stranded DNA molecule is copied to produce two identical DNA molecules.
DNA replication is one of the most basic processes that occurs within a cell. Each time a cell divides, the two resulting daughter cells must contain exactly the same DNA as the parent cell. To accomplish this, each strand of existing DNA acts as a template for replication.
How is DNA replicated? DNA replication occurs in three major steps:
1. the opening of the double helix and separation of the DNA strands
2. the priming of the template strand
3. the assembly of the new DNA segment
During separation, the two strands of the DNA double helix uncoil at a specific location called the
…show more content…
First, an initiator protein unwinds a short amount of the double helix. After this, the protein known as helicase, attaches to and then breaks up the hydrogen bonds between the bases of the DNA strands, therefore pulling them apart. As the helicase moves along the DNA molecule, it continues breaking the hydrogen bonds and also separating the two polynucleotide chains.
Meanwhile, as the helicase separates the strands, another enzyme called primase briefly attaches to each strand and creates a place where the replication can begin. This place is full of nucleotides, called a primer.
How are DNA strands replicated?
At the beginning at the primer sequence, the DNA polymerase attaches to the original DNA strand and begins assembling a complementary strand.
After the primer is in place on a single, unwound polynucleotide strand, the DNA polymerase wraps itself around that strand, and it attaches new nucleotides to the exposed nitrogenous bases. The polymerase assembles a new DNA strand on top of the existing one.
As the DNA polymerase makes its way down the unwound DNA strand, it relies on the floating nucleotides that surround the existing strand to build the new strand. The nucleotides that make up the new strand are paired with partner nucleotides in the template
The two modes of analysis that will be used to identify an unknown insert piece of DNA would be plating the transformation cells onto LA plates that have either ampicillin or chloramphenicol and PCR. We will use the PCR thermocycler to denature the restriction enzymes that were specifically used to assimilate the vector DNA. It is important to use the PCR thermocycler because denaturation of the restriction enzyme will prevent the restriction enzyme from cutting the vector DNA, after the insert DNA has assimilated to the vector DNA. After the addition of specific primers that complement the base pair to its corresponding target strand, PCR will be used. Subsequently, Taq polymerase will be used to determine whether the insert DNA has been properly assimilated to the vector DNA. Within this specific situation, the target strand will be the insert DNA. After we let the PCR thermocycler run for approximately 2 ½ hours, we will then put our PCR products in the gel and run the gel to completion. After the gel has run to completion, we will then take a photograph of the gel using the UV transilluminator with the assistance of our TA. If the insert DNA was properly assimilated to the vector DNA, then our corresponding gel photo would have one band. After the cells have been transformed, we would g...
The adage is a symphony. The way the PCR method works is by first mixing a solution containing the DNA, DNA polymerase primers, and certain nucleotides.... ... middle of paper ... ...
DNA is the genetic material found in cells of all living organisms. Human beings contain approximately one trillion cells (Aronson 9). DNA is a long strand in the shape of a double helix made up of small building blocks (Riley). The repeat segments are cut out of the DNA strand by a restrictive enzyme that acts like scissors and the resulting fragments are sorted out by electrophoresis (Saferstein 391).
Miller, Kenneth R. and Joseph S. Levine. “Chapter 12: DNA and RNA.” Biology. Upper Saddle River: Pearson Education, Inc., 2002. Print.
"Polymerase Chain Reaction (PCR) Fact Sheet." National Human Genome Research Institute. 10 Dec. 2007. National Institutes of Health. .
In telophase, these separate chromatids uncoil to become chromosomes. This division produces two identical cells.
DNA methylation primarily occurs within sites in the DNA sequence known as CpG dinucleotides, which is a 2 base pair sequence involving a Cytosine bonded to a Guanine by a phosphodiester bond.
In bacteria, RNA polymerase attaches right to the DNA of the promoter. You can see how this process works, and how it can be regulated by transcription factors, in the lac operon and trp operon videos.
In the Prophase, chromosome as double threads joined at centromere and then shorten and thicken. Then nucleolus and nuclear membrane disappears. Fibrils of astral rays extend across forming mitotic spindle. After that centriole divides into two.
Precise chromosomal DNA replication during S phase of the cell cycle is a crucial factor in the proper maintenance of the genome from generation to generation. The current “once-per-cell-cycle” model of eukaryotic chromosome duplication describes a highly coordinated process by which temporally regulated replicon clusters are sequentially activated and subsequently united to form two semi-conserved copies of the genome. Replicon clusters, or replication domains, are comprised of individual replication units that are synchronously activated at predetermined points during S phase. Bi-directional replication within each replicon is initiated at periodic AT-rich origins along each chromosome. Origins are not characterized by any specific nucleotide sequence, but rather the spatial arrangement of origin replication complexes (ORCs). Given the duration of the S phase and replication fork rate, adjacent origins must be appropriately spaced to ensure the complete replication of each replicon. Chromatin arrangement by the nuclear matrix may be the underpinning factor responsible for ORC positioning. The six subunit ORC binds to origins of replication in an ATP-dependent manner during late telophase and early G1. In yeast, each replication domain simply contains a single ORC binding site. However, more complex origins are characterized by an initiation zone where DNA synthesis may begin at numerous locations. A single round of DNA synthesis at each activated origin is achieved by “lic...
A chromosome is made up of two identical structures called chromatids. The process of nuclear division is called interphase; each DNA molecule in a nucleus makes an identical copy of itself. Each copy is contained in the chromatid and a characteristic narrow region called the centromere holds the two chromatids together. The centromere can be found anywhere along a chromosome but the position is the characteristic for a particular chromosome. Each Chromatid contains one DNA molecule. DNA is the molecule of inheritance and is made up of a series of genes. The fact that the two DNA molecules in the sister chromatids, and hence their genes, are identical is the key to precise nuclear division.
DNA (deoxyribonucleic acid) is a self-replicating molecule or material present in nearly all living organisms as the main constituent in chromosomes. It encodes the genetic instructions used in the development and functioning of all known living organisms and many viruses. Simply put, DNA contains the instructions needed for an organism to develop, survive and reproduce. The discovery and use of DNA has seen many changes and made great progress over many years. James Watson was a pioneer molecular biologist who is credited, along with Francis Crick and Maurice Wilkins, with discovering the double helix structure of the DNA molecule. The three won the Nobel Prize in Medicine in 1962 for their work (Bagley, 2013). Scientist use the term “double helix” to describe DNA’s winding, two-stranded chemical structure. This shape looks much like a twisted ladder and gives the DNA the power to pass along biological instructions with great precision.
The process of cell division plays a very important role in the everyday life of human beings as well as all living organisms. If we did not have cell division, all living organisms would cease to reproduce and eventually perish because of it. Within cell division, there are some key roles that are known as nuclear division and cytokinesis. There are two types within nuclear division. Those two types being mitosis and meiosis. Mitosis and meiosis play a very important role in the everyday life as well. Mitosis is the asexual reproduction in which two cells divide in two in order to make duplicate cells. The cells have an equal number of chromosomes which will result in diploid cells. Mitosis is genetically identical and occurs in all living
Secondly the gene has to be cut from its DNA chain. Controlling this process are many restriction endonucleases (restriction enzymes). Each of these enzymes cut DNA at a different base sequence called a recognition sequence. The recognition sequence is 6 base pairs long. The restriction enzymes PstI cuts DNA horizontally and vertically to produce sticky ends.
As a result of the viral infection by two viruses, both viruses would replicate, producing four homologous chromosomes which would then be 'handcuffed' together in such a way preventing further replication.(14) Viral segregation mechanisms ensure that the viral pairs would segregate to either pole of the cell during replication.(14) After a round of replication, there would still be two viral chromosomes attached to each other and viral replication could not continue.(14) As a result, the cell would enter a second replication cycle to segregate the individual viral chromosomes into the host cells.(14) This