Chemical Testing Lab Report

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Identifying macromolecules by using chemical testing in an unknown solution
Leonardo Vargas, Hyat Farraj, Naraly Sosa, Alain Greenwald
To be able to identify what kind of macromolecules a solution has, it has to be tested to be able to identify what macromolecules are present in the solution. Here we used only one test for each macromolecule that we are testing for. We tested for RNA, DNA, protein, sugar, starch, and lipid macromolecules. Using these kinds of test we were able to figure out, that we can detect what kind of macromolecules are present in a solution almost with full accuracy.
To be able to understand what kind of functions are going on at a molecular level on a cell, you have to first know what macromolecules form the structure you are testing. To do these kinds of studies with chemical testing you have to be able to isolate the structure you are testing for.
One scientist who was doing these kinds of testing early on was Friedrich Miescher, who “wanted to determine the …show more content…

5 test tubes were used for this lab, each label 1-5. Then we added 1ml of assign solution for each test tube. Tube 1 had DNA, 2 had RNA, 3 had the nucleic acids from the liver extract supernatant, 4 had water, and 5 had the unknown. 2ml of Dische’s reagent was then added to each test tube. All of the test tubes are then place in boiling water. After 15 minutes in the boiling water, we recorded the color of each of the different test tubes in a table.
Orcinol Test. 5 clean test tubes were label from 1-5. 1ml of different solutions were then added to different tubes. Tube 1 had DNA, 2 had RNA, 3 had nucleic acids, 4 had water, and 5 had the unknown. 1.5ml of Orcinol A was then added to each test tubes, and then 2 drops of Orcino B were also added to each of test tubes. The contents of each tube were then mixed, after mixing, the tubes were then placed in boiling water for 15 minutes with marbles covering the tubes. Colors of the solutions were then recorded in a

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