Abstract (½ pagina)
This lab report dealt with the analyzation and transmission of genetic traits in monohybrid and dihybrid crosses using Caenorhabditis. Mutations will be either dominant or recessive or X-linked or autosomal. Where using a sterile pick you will pick certain worms and place them in a new petri dish for them to reproduce and observe new progenies, mutations and different crosses.
should provide a summary of the entire lab report so that a reader could get the “gist” of your lab report before they read it. It should include the purpose of your experiment, brief
Overview of the experimental approach, and primary results and conclusions.
Intro (1.5 – 2 paginas)
As learned in genetics, genetic traits are carried onto offspring by either being dominant or recessive which can be either displayed in their phenotype or be shown in their genotype. Here is where we get a deeper understanding of such. Since the objective was to analyze the transmission of genetic traits in monohybrid and dihybrid progenies using Caenorhabditis elegans as a model organism. Which will be used to determine
…show more content…
Firstly, Stock 1 was the wild type, Stock 2 was the dumpy, and stock 3 was the uncoordinated worms. Worms could be one of the two: males, or hermaphrodites/females. Secondly, Hermaphrodites being 30% males and males and hermaphrodites being 50% males, from a mutation. From the three different stocks, there were some visual observations that could be made. Thirdly, Wild type worms were males, these worms moved in an s shape motion. Stock 2 being dumpy, these worms were fat and short but also moved in an s shape, and finally stock 3. Stock 3 was the uncoordinated were strictly females/hermaphrodites. These worms are considered uncoordinated since there is a mutation in the nervous
Sordaria fimicola is a species of microscopic fungus that is an Ascomycete and are used to test for genetic variation in the lab setting (Sordaria fimicola: A Fungus used in Genetics, Volk). These organisms are what are called model organisms, or species that has been widely studied usually because it is easy to maintain and breed in a laboratory setting and has particular experimental advantages (Sordaria fimicola, Volk). S. fimicola, because it is in the Ascomycota phylum, have a distinguishing reproductive structure called the ascus, which is surrounded by the perithecium. This cylindrical sac-like structure houses 8 haploid spores; created through meiosis to produce 4 haploid spores and then mitosis to make 8 (Lab Manual, pg. 59-68). Based on the genotype they will vary in order and color. There are 3 different ratios that can arise from the 8 ascospores: 4:4, 2:2:2:2, and 2:4:2 (black/wild type and tan coloration). The 4:4 ratio suggests that no crossing over had occurred because there is no difference in order of the color parents that were mated. The two other ratios suggest genetic recombination, or crossing over, because of the
to get an idea of how I would do my real experiment and what apparatus
The idea of the project was to experiment breeding Drosophila Melanogaster (fruit fly) to figure out if certain genes of that species were sex linked or not (autosomal). A mono-hybrid cross and di-hybrid cross was performed. For the mono-hybrid cross, white eyed female and red eyed male were placed in one vial for them to reproduce. For the di-hybrid cross, red eyed and normal winged flies and sepia eyed and vestigial winged flies were placed in their vial to reproduce. In the mono-hybrid cross the results expected were within a 1:1:1:1 ratio. Expected results similar to the expected desired null hypothesis proposed with what the F1 parental generation breeds. The potential results would have had to have been within the ratios of 9:3:3:1. The results were clear and allowed the null hypothesis to be correct. The white eyed gene in the fruit flies is sex linked. Sepia eyes and vestigial wings are not sex linked and are examples of independent assortment.
In this experiment, Mendelain Models are observed. The purpose of the experiment is to understand how traits are passed from one generation to the other as well as understanding the difference between sex linked and autosomal genes. One particular trait that is observed in this experiment is when a fly is lacking wings, also known as an apterous mutation. In this experiment, we will determine whether this mutation is carried on an autosomal chromosome or on a sex chromosome. The data for this experiment will be determined statistically with the aid of a chi-square. If the trait is autosomal, then it will be able to be passed to the next generation on an autosomal chromosome, meaning that there should be an equal amount of male and
The major topic of this experiment was to examine two different crosses between Drosophila fruit flies and to determine how many flies of each phenotype were produced. Phenotype refers to an individual’s appearance, where as genotype refers to an individual’s genes. The basic law of genetics that was examined in this lab was formulated by a man often times called the “father of genetics,” Gregor Mendel. He determined that individuals have two alternate forms of a gene, referred to as two alleles. An individual can me homozygous dominant (two dominant alleles, AA), homozygous recessive, (two recessive alleles, aa), or heterozygous (one dominant and one recessive allele, Aa). There were tow particular crosses that took place in this experiment. The first cross-performed was Ebony Bodies versus Vestigle Wings, where Long wings are dominant over short wings and normal bodies are dominant over black bodies. The other cross that was performed was White versus Wild where red eyes in fruit flies are dominant over white eyes.
List of the tests to be conducted, material to be tested, the location of sampling, the organization’s name that will perform the test, and the frequency of testing.
E. Wrap up the conclusive section with a closing note that provides brief information about a unique fact and/or behavior of your chosen organism.
The exercise involved a series of ‘mating’ events resulting in 6 generations. Each mating event produced offspring with ‘possible’ newly inherited traits. The idea of ‘chance’ was included through simple coin tosses. Also, ideas of selection and mutations were introduced into the ‘gene pool’, which presented a deeper and more clear understanding of Mendelian inheritance and the Hardy-Weinberg equilibrium. Upon reaching the third generation, A B1 mutant allele was introduced to the blue locus-influencing fin shape and a G1 mutant allele was introduced to the green locus-influencing Mouth
Results. Write up your result (the letters and the full typology) and your interpretation of the meaning of your result (do not copy and paste what the assessment gives you).
First we placed the slide under the simple microscope and observed it at ten times magnification level. We each took turns looking. We then copied them as drawings into our Cornell notebooks. After that we changed the magnification to fifty times and observed the slide. We each took turns looking. We then copied what we saw into our Cornell notebooks. After that we changed the magnification level to sixty times and we each took turns looking at the slide. We then copied what we saw into our Cornell notebooks and sat down together. When we were all done drawing we spoke about what we seen and gave each other ideas on how to write our Lab Reports.
Genetically influenced traits tend to be polygenic in character, involving many genes acting in concert to produce a certain response. Therefore, association of one gene with one behavior is usually only partially conclusive. Behavior depends on the interaction of multiple gene sequences with environmental influences. ...
more than half the variation was found to be due to heredity. Among these traits were
Do not cram lots of detail, diagrams, figures, evidence, references etc., into the main body of the report. Index and attach these references as appendices at the end of the report.