Alcian Blue Staining System Essay

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Figure 4.1 (above): 4X magnification (taken above the light source) light microscope image of a late gestation period mouse embryo, stained with Haematoxylin/Eosin and Alcian Blue
Figure 4.1 depicts the macro section of a mouse embryo, the Alcian Blue stain indicates that there is connective tissue present. The Nasal cartilages is supposedly made up of Hyaline cartilage, however the staining method and its appearance, is hinted towards elastic cartilage. Although not visible or past the visible stage, Neural stem cells would have been present for the brain development, alongside Mesenchymal stem cells for cartilage and bone development (Alcian Blue stain).
Figure 4.2 (left): 10X magnification light microscope image of a late gestation period mouse embryo, stained with Haematoxylin/Eosin and Alcian Blue. …show more content…

Within the ear canal, there appears to be a blood vessel present, which is stained in red. Coincidentally and potentially, due to a blood vessel being present next to the ear canal, the calcification of the ear cartilage may be due to vessel derived stem cells (6). The Alcian Blue stain highlights the chondrocytes that are in the process of calcification.

Figure 4.3 (above): 40X magnification light microscope image of a late gestation period mouse embryo, stained with Haematoxylin/Eosin and Alcian Blue.
Figure 4.3 depicts the ossification and calcification activity of the nasal cartilage. It could potentially be calcified chondrocytes or chondrocytes lacunae that have not yet calcified. There may be presence of Mesenchymal stem cells or vessel derived stem cells due to a blood vessel being developed near the nose cavity, which differentiate into chondrocytes

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