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Diffusion in the plasma membrane
Consequentialist analysis
Diffusion in the plasma membrane
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Within activity one our group had made multiple hypotheses such as due to urea’s molecular weight, it would defuse through the 20 Molecular Weight Cut off (MWCO) membrane successfully, yet slowly. We also made the hypothesis that both albumin and glucose would successfully be able to diffuse through the 200 MWCO membrane. We assumed that this would be found to be true because of factors such as glucose being a monosaccharide, and albumin being a protein with approximately 607 amino acids.
After conducting our experiment, when looking at our data sheet we found that our hypothesis involving albumin being able to diffuse through the 200 MWCO was not proven. albumin was the one solute that was not able to pass through any of the membranes. This could have been due to albumin being the molecule with the largest molecular weight amongst the other molecules involved. This included sodium chloride, urea, and glucose. In spite of this, glucose was able to successfully pass through specific membranes. In spite of this, glucose is an example of a molecule that may require
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We found our hypothesis to be true when looking back on our recorded date. Within the data sheet we observed that the movements when the glucose transport rate was constantly increasing as we increased the carriers. An example of this would be when the carrier rate was increased to 700 carriers, and as a result the transport rate had successfully reached .0031 in a drastically shorter period of time. As opposed to previous trials we performed with fewer protein carriers such as 100, which led to a slower glucose transportation rate. Increasing both the glucose concentration as well as the number of membrane carriers are also two variables that had played key roles in increasing the rate of facilitated diffusion of glucose as
What is the transducer in our sensor scheme (in the assay you used)? What is the most common transducer for commercial glucometers (such as what you would buy at a drug store)? May have to research this. (5 points)
Data table 1 Well plate Contents Glucose concentration A 3 drops 5% sucrose + 3 drops distilled water Negative B 3 drops milk+3 drops distilled water Negative C 3 drops 5% sucrose +3 drops lactase Negative D 3 drops milk +3 drops lactase 15+ E 3 drops 20% glucose +3 drops distilled water 110 ++ Questions B. In this exercise, five reactions were performed. Of those reactions, two were negative controls and one was a positive control.
We then took 1ml of the 10% glucose solution again using the glucose rinsed pipette and added it to test tube 1, we then filled the H2O rinsed pipette with 9ml of H2O and added it to test tube one; making 10ml of 1% solution.
One of the most primitive actions known is the consumption of lactose, (milk), from the mother after birth. Mammals have an innate predisposition towards this consumption, as it is their main source of energy. Most mammals lose the ability to digest lactose shortly after their birth. The ability to digest lactose is determined by the presence of an enzyme called lactase, which is found in the lining of the small intestine. An enzyme is a small molecule or group of molecules that act as a catalyst (catalyst being defined as a molecule that binds to the original reactant and lowers the amount of energy needed to break apart the original molecule to obtain energy) in breaking apart the lactose molecule. In mammals, the lactase enzyme is present
This study observed the standard and routine metabolic rates and swimming activities of nurse sharks. Nurse sharks use buccal pumping to rest on the sea floor. This sedentary behavior had not yet been studied in relation to metabolic rates before this study. This study also is one of few that observed the effcts of temperature on metabolism in sharks. By assessing the relationship between routine metabolism and ecology, a more precise understanding of the nurse sharks daily energy requirements could be obtained.
This graph shows that as enzyme concentration increases absorption also increases. In this case absorbance can be used to measure the enzyme’s activity, the higher the absorption the higher the activity. Since absorption increases as enzyme concentration increases, enzyme activity is promoted by increased enzyme concentrations. After a certain point enzyme activity would fail to increase as a result of increased enzyme concentration since there wouldn’t be enough substrate for all of the enzymes to react with.
This occurs when special carrier proteins carry solutes dissolved in the water across the membrane by using active transport. When the concentration gradient can not allow travel from one side of the membrane to the other fast enough for the cell’s nutritional needs, then facilitated diffusion is used. The transport protein is specialized for the solute it is carrying, just as enzymes are specialized for their substrate. The transport protein can be
In order to test this theory the researchers injected the GluRs into the blood stream of a normal
...s a component monomer of starch. As a monomer as opposed to a polymer, it is much smaller and would thus be able to cross the plasma membrane. However, glucose is a larger solute than the component ions of salt, thus meaning that simple diffusion would not be sufficient. Instead, facilitated diffusion would be needed to transport the glucose. However, in the dialysis tubing, there is no facilitated transport like there is for the plasma membrane. Thus, the glucose may pass through the dialysis tubing, but it would not be due to transport, but the artificial enlargement of the passages in the dialysis tubing. Water would move freely inside and outside of the cell, however, because there is a greater solute concentration inside the cell, the water would diffuse through osmosis into the cell model, increasing the final mass of the dialysis tubing and causing cytolysis.
Dialysis depends on dispersion amid which the portability of solute particles between two fluid spaces is confined, for the most part as indicated by their size. (In infrequently utilized adaptations of dialysis, confinement of dissemination by means of
What is diabetes? Should I be worried about getting it? What food can diabetics eat? Can they live as long as non-diabetics? Those were some of the questions that swarmed in my head when two of my close relatives were diagnosed with diabetes. After my relatives had been diagnosed, my whole extended family changed in how we ate and looked at food. I became very aware of this disease and thought I knew a little bit of what it was, but still had a lot of questions. I now feel like I have the knowledge of exactly what diabetes is, how to deal with it, and most important, preventing it.
The mixture for that table’s flask was 15 mL Sucrose, 10 mL of RO water and 10 mL of Yeast, which the flask was then placed in an incubator at 37 degrees Celsius. In my hypothesis for comparison #4 the measurements would go up again with every 15 min. intervals because of the high tempeture and also be higher that then Controlled Table’s measurements. Hypothesis was right for the first part but was wrong for the second part of the comparison, the measurements did increase in the table’s personal flask but the measurements did not get higher than the Controlled Table’s measurements, see chart below. In conclusion, I feel that the substitution of glucose for sucrose made the enzymes work just as hard as the Controlled Table’s flask but just not as much because sucrose was too strong for the enzymes to
Activity 3: Investigating Osmosis and Diffusion Through Nonliving Membranes. In this activity, through the use of dialysis sacs and varying concentrations of solutions, the movement of water and solutes will be observed through a semipermeable membrane. The gradients at which the solutes NaCl and glucose diffuse is unproportional to any other molecule, therefore they will proceed down their own gradients. However, the same is not true for water, whose concentration gradient is affected by solute ...
According to the graph on amylase activity at various enzyme concentration (graph 1), the increase of enzyme dilution results in a slower decrease of amylose percentage. Looking at the graph, the amylose percentage decreases at a fast rate with the undiluted enzyme. However, the enzyme dilution with a concentration of 1:3 decreased at a slow rate over time. Additionally, the higher the enzyme dilution, the higher the amylose percentage. For example, in the graph it can be seen that the enzyme dilution with a 1:9 concentration increased over time. However, there is a drastic increase after four minutes, but this is most likely a result of the error that was encountered during the experiment. The undiluted enzyme and the enzyme dilution had a low amylose percentage because there was high enzyme activity. Also, there was an increase in amylose percentage with the enzyme dilution with a 1: 9 concentrations because there was low enzyme activity.
The purpose of this lab was to see firsthand the diffusion of a substance across a selectively permeable membrane. Diffusion is the movement of molecules from an area of high concentration to an area of lower concentration until both concentrations are equal, or as you could more professionally call it, equilibrium. This concept is one that we have been studying in depth currently in Biology class.