After Fly Genetics Lab Report

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In this experiment, we are analyzing qualitive data of targeted characteristics of offspring Drosophila of a female Drosophila with a Gal 4 driver and male Drosophila with DNA containing UAS and genome needed for RNAi. If there is a deformation of such targeted characteristics in the offspring, in this experiment the eyes or wings, this indicates a possible disruption of metabolic genes.
When flies are crossed, the expression of a targeted gene can be present in four different ways; it can be expressed only during fly development, only after fly development, both during and after fly development, or neither during nor after fly development. We are interested in finding offspring that express the targeted gene only during development.
Of the RNAi …show more content…

For this process to begin, the genome of the strand of DNA must form a specific pattern. If a line was draw down the very center of the DNA sequence, every base of the same distance away from the center line must be matching based pairs. To illustrate this concept, a diagram bound to the same rule with ten base pairs would have matching base pairs at numbers 5 and 6, 4 and 7, 3 and 8, 2 and 9, and 1 and 10.
When this pattern occurs, the genome sequence of the RNA derived from the previously described DNA can bind to itself. This creates a bend in the genome, and the transcribed RNA becomes double-stranded. This structure is like that of a virus, and the cell regards it as such. Therefore, just as it would a virus, the cell looks for matching sequences and destroys them. This disallows the gene to be expressed.
Of the flies containing Gal 4, we are utilizing virgin females in this experiment. When Gal 4 is present in a female fly, that fly’s offspring has a disposition to have a phenotype associated with that Gal 4. In this experiment, we are breeding and crossing three different types of Gal 4 females; Gal 4 eya, Gal 4 GMR, and Gal 4

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