The process of photosynthesis is present in both prokaryotic and eukaryotic cells and is the process in which cells transform energy in the form of light from the sun into chemical energy in the form of organic compounds and gaseous oxygen (See Equation Below). In photosynthesis, water is oxidized to gaseous oxygen and carbon dioxide is reduced to glucose. Furthermore, photosynthesis is an anabolic process, or in other words is a metabolism that is associated with the construction of large molecules such as glucose. The process of photosynthesis occurs in two steps: light reactions and the Calvin cycle. The light reactions of photosynthesis take place in the thylakoid membrane and use the energy from the sun to produce ATP and NADPH2. The Calvin cycle takes place in the stroma of the chloroplast and consumes ATP and NADPH2 to reduce carbon dioxide to a sugar. Light Energy + 6 H2O + 6 CO2 C6H12O6 + 6 O2 In this laboratory experiment, the rate of photosynthesis was measured through the use of the “floating leaf disk technique.” The leaf disks were placed into a syringe and the O2 and CO2 in the mesophyll layers of the leaves were removed and then replaced with sodium bicarbonate or water, causing the leaves to sink to the bottom of the container. If one determines the number of leaf disks rising to the top as a result of an increase in oxygen gas in the mesophyll cells, then the rate of photosynthesis is able to be measured because O2 is a product of photosynthesis. The first step of this experiment was a feasibility study of the variance in the photosynthetic activity of the leaf disks in both water and bicarbonate solutions. After five minutes of light exposure, all of the leaf disks in the bicarbonate solution (10 disks) had ... ... middle of paper ... ...d have led to inaccurate measurements because the leaves would not have been able to perform photosynthesis and rise to the surface. In addition, the air in the mesophyll layers of the leaves may not have been properly cleared by the syringe, which would have made it easier for the leaf disks to float to the surface because the leaf would have to perform a lower rate of photosynthesis. All of these errors may have been prevented by having a partner check over sections of the experiment to ensure accurate results and repeating the experiment numerous times to account for random error. Moreover, a future experiment is to determine the effect that the distance between the lamp and the solution has on the rate of photosynthesis. Several experiments with a similar setup to this experiment that vary the distances between the lamp and solution could be used to test this.
The experiment was conducted using carbon dioxide to see how it affected the rate of photosynthesis in spinach leaves. Carbon dioxide should increase the rate of photosynthesis because there will be more carbon dioxide, a reactant in the photosynthesis formula.
Experiment #1: The purpose of this experiment is to investigate the effects of baking soda and light intensity on the rate of photosynthesis of green spinach leave through the observation of floating disk.
The Effect of Light Intensity on the Rate of Oxygen Production in a Plant While Photosynthesis is Taking Place
= > [CH2O} + O2 + H2O, This shows that when the light intensity is increased the rate of reaction will be more quicker he only anomalous result there was, is the one in the 100 watt result the reading after 5 minutes is anomalous because it does not follow the predicted pattern of increasing in the production of gas because it is lower I know from my own knowledge of photosynthesise that when the light intensity is increased the rate of reaction will be more quicker because many plants and trees photosynthesise quicker in stronger light and photosynthesise slower in dimly lit places. The chlorophyll absorbs light energy and enables it to be used by the plant for building up sugar. The overall effect is that energy is transferred from sunlight to sugar molecules.
Doing experiments and labs with photosynthesis can be a challenge. The floating leaf disk assay technique works well for doing labs. For this experiment you will need baking soda, a plastic syringe, leafs, hole punch, plastic cups, dilute liquid soap, timer and a light source. Prepare 1/8 of a teaspoon of baking soda and 300 ml of water. The baking soda acts as an alternative source of carbon dioxide for photosynthesis. You then add 1 drop of dilute soap to the solution, the soap wets the outside of the leaf allowing the solution to be drawn into the leaf. Avoid having suds if the solution has suds when on the leaf put more bicarbonate into the solution. You then cut ten or more leaf disks for each time you want to do the experiment. The leaf
... the floating discs easier. If this experiment is conducted again, I will have corrected these errors and been more careful. In a follow up experiment, the rate of photosynthesis can be calculated directly by the production of oxygen using oxygen probes. I could also calculate the rate increase in biomass in the plants. However in doing so, I may need to increase my sample size from 10 to perhaps 50 or 100 to generate more accurate results. In a follow-up experiment I plan on measuring the effects of temperature on photosynthesis. Low temperature slow down rates of photosynthesis by slowing down enzymatic processes. High temperatures denature enzymes and have similar results. With this knowledge I would explore how do rapid fluctuations in temperature impact plant growth, and is there a plant species resistant to these changes? Only further research can answer this.
Photosynthesis is, “the process by which plants convert light energy from the Sun into chemical energy in the form of carbohydrates” thus producing, “food for all living organisms, directly or indirectly” (Zheng). Photosynthesis has been examined in thousands of different ways. Many of these experiments include studying the rate of photosynthesis and pigment accumulation by obtaining plants and then stressing their light and nutrient intake (Okunlola and Adekunle). Photosynthetic pigments reflect and absorb different wavelengths of visible light based off their polarity. In this experiment, we studied photosynthetic pigments, first, by determining polarity and then, by measuring the amount of light of a given wavelength that a pigment absorbs. We used two methods in this experiment, chromatography and spectrophotometry. Chromatography “is a method used to separate mixtures of substances into their components” (lab book) and spectrophotometry is the use of a spectrophotometer to measure transmittance of light through a liquid. We used our knowledge of polarity to predict that since the least polar pigments move the most, pigment 1 is chlorophyll b, pigment 2 is chlorophyll a, pigment 3 is an anthocyanin, pigment 4 is a xanthophyll, and since most polar pigments move the least, pigment 5 is
C3 photosynthesis is the ancestral form of photosynthesis and is present in the majority of plant species (Sage, Sage & Kocacinar 2012). In this process CO2 enters the mesophyll cell (mc) via openings in the epidermis called stomata and diffuses into the into the chloroplast where it enters the Calvin cycle (Raven, Evert & Eichhorn 2013). The Calvin cycle is made up of three stages: 1. Carboxylation of Ribulose-1,5-bisphosphate (RuBP). In which a molecule of CO2 is covalently bonded to a molecule of RuBP forming the first stable intermediate 3-Phosphoglycerate. It is from this 3 carbon product that the name C3 photosynthesis comes from. This reaction catalysed by the enzyme Ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) 2. Reduction of 3-phosphoglycerate to the carbohydrate glyceraldehyde-3-phosphate, utilizing the ATP and NADPH formed in the light harvesting reactions. 3. Regeneration of the initial substrate RuBP (Taiz & Zeiger 2006). These stages encompass thirteen different reactions the net result of which is:
Investigating the Effect of Light Intensity on Photosynthesis in a Pondweed Aim: To investigate how the rate of photosynthesis changes at different light intensities, with a pondweed. Prediction: I predict that the oxygen bubbles will decrease when the lamp is further away from the measuring cylinder, because light intensity is a factor of photosynthesis. The plant may stop photosynthesising when the pondweed is at the furthest distance from the lamp (8cm). Without light, the plant will stop the photosynthesising process, because, light is a limited factor. However once a particular light intensity is reached the rate of photosynthesis stays constant, even if the light intensity is the greatest.
They are the same reactions, but occur in reverse. In photosynthesis, carbon dioxide and water yield glucose and oxygen respiration, process glucose and oxygen yield carbon dioxide and water, catabolic pathway process which requires or contains molecular oxygen for the production of adenosine triphosphate. This three step aerobic respiration cycle occurs in the cytoplasm and in the organelles called mitochondria. Within this process, cells break down oxygen and glucose in a storable form called adenosine triphosphate or ATP. This cellular respiration or sometimes called an exothermic reaction is similar to a combustion type reaction whereby the cell releases energy in the form heat but at a much slower rate within a living cell.
For my one research task project I have decided to see if light is necessary for photosynthesis to take place in green plants. I choose this topic because I wanted to see if light necessary for photosynthesis is really. It is also a very interesting topic because most living things need light to function, survive and grow. Photosynthesis is the physic-chemical process by which green plants use light energy to photosynthesis. When Photosynthesis occurs it takes in the co2 from the atmosphere and releases oxygen as a bi product. In addition the plants provide energy for humans.
Hypothesis: The rate of photosynthesis in the water plant hydrilla will change as the rate of carbon dioxide changes.
Photosynthesis and cellular respiration help sustain life on planet earth as both are metabolic processes in their own way. Photosynthesis is the process by which plants and other organisms use energy from the sun to form glucose from water and carbon dioxide. From there, glucose is then converted to ATP by way of cellular respiration. To convert nutrients that are biochemical energy into ATP, a process such as cellular respiration that has reactions needs to take shape in the cell of an organism, releasing waste products at the same time. For the continuous energy cycle that tolerates life on Earth as we know it Photosynthesis and Cellular respiration very essential. They have a few stages where energy and various connections occur within the eukaryotic cell. Cellular respiration takes place in the lysosome, an organelle that is found in the cytoplasm of eukaryotic cells. It uses enzymes to break down biomolecules including proteins, nucleic acids, carbohydrates, and lipids. Photosynthesis involves the chloroplasts, which contain pigments that absorb the sunlight and then transfigure them to sugars the plant can use. Those specific processes are crucial in how far and diversified evolution has
The Importance of Photosynthesis and What it Does for Life According to scientists, life is “the condition that distinguishes animals and plants from inorganic matter, including the capacity for growth, reproduction, functional activity, and continual change preceding death, also the way of life of a human being or animal. ”("Life,”) In order for one to have life, one must have the nine characteristics to be considered a living thing. These nine characteristics are: all living things are made up of cells, living things are able to reproduce, living things use energy, maintain homeostasis, respond and adapt to the environment, grow and develop, have a life span, evolve over time, and are interdependent. All of the nine characteristics have one thing in common, something that is needed for all living things to work, even if they do not know it.
Photosynthesis is a cycle plants go through converting light into chemical energy for use later. Photosynthesis starts in the chloroplasts, they capture chlorophyll, an important chemical needed for photosynthesis. Chloroplasts also take water, carbon dioxide, oxygen and glucose. The chlorophyll is taken to the stroma, where carbon dioxide and water mix together to make