such as ethanol was purposed from Decon Laboratories (USA), acetone was purposed from Fisher Chemicals, USA, and isopropanol was purchased from Macron Fine Chemicals, USA. Chloroauric acid, hydrogen chloride, nitric acid, sodium phosphate monobasic, and sodium phosphate bibasic were purchased from Sigma-Aldrich, USA. L-ascorbic acid were purchased from Sigma-Aldrich, USA Gold micro wire (~0.01 mm diameter) was purchased
MTT assay was used to analyze cell viability of MCF 10A, MCF 7 and MDB-MB 231 cell lines in control and 10mM inorganic phosphate treated groups. MTT assay is a colorimetric assay for evaluating cell metabolic activity. MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) is a yellow aqueous tetrazolium dye. In living cells with active mitochondria, the mitochondrial enzymes such as succinate dehydrogenase (NADPH dependent oxidoreductase enzyme) reduce this dye to formazan, a purple-colored
The SRBC (sheep’s red blood cells) were used as an antigen to interact with rabbit’s serum to form antibody-antigen lattice complex in the micro titre plate v-shaped wells. Row A is as a standard to compare with rest of the well. PBS (Phosphate buffered saline) was used as a medium for the antibody and antigen to interact. Three different samples of rabbit’s serum (sera 1, 2 and 3) were prepared. Sera 1 was from rabbit that was not exposed to sheep’s red blood cells (pre-immunised), sera 2 was
Injectable Hydrogels: Processing and Application Introduction Hydrogels are defined as networks made up of polymer chains which are hydrophilic. They are in some cases found as a solid dispersed in a liquid. where water acts as the medium dispersed. Hydrogels are natural, highly porous or artificial polymeric networks. The hydrophilic structure allows hydrogels to hold the significant amount of water within their three-dimension structural systems. The extensive employment of hydrogel products in
Locating the Dopamine Transporter via Immunostaining and Colocalization Taryn Nurse+, Kadija King+, Dasia McClain+, Jahmel Fowler+, Phyllis Freeman+, and Shawn Goodwin*. Life and Physical Science Department, Fisk University+ and Department of Biology. Meharry Medical College School of Graduate Studies and Research*, Nashville, TN, 37208. Introduction (Dasia) The focus of this experiment was to identify the Dopamine Active Transporter (DAT), known as SLC6A3, which is located on the 5th chromosome
Introduction Cellular prion proteins are normally occurring glycoproteins found on the outer surface of neuronal cells. They are also expressed by most other cells found in the body. Prion proteins are attached to the plasma membrane by a C-terminus glycosyl-phosphatidylinositol (GPI) anchor. The prion protein exists in two conformational states: a cellular α-helix-rich isoform (PrPc) and the prion disease-associated β-sheet isoform (PrPsc). In humans, PrPc is composed of 253 amino acids and is
Immunosensors make use of antigen-antibody interactions [1, 2] to detect a wide range of analytes which are of great interest in medical diagnostics, environmental analysis, and forensic medicine [3, 4], including pathogens [5], drugs [6], bacteria [7], toxins [8], and biomarkers [9]. Overall, immunosensors employ the same chemical approach of earliest immunoassays, but offer quicker and simpler analytical procedures that may be conducted at the point-of-care [10]. Immunosensors use an antibody immobilized
as Sabouraud's broth. The pad is then placed on the affected lesions in the mucosal cavity for 30 seconds and then transferred to an agar for culture. The last method is the oral rinse technique which require the patient to hold a sterile phosphate buffered saline in the mouth for one minute. The solution is then inoculated on an agar media at 37oC for 24-48 hours (1). Candida species... ... middle of paper ... ...icrobes Infect (2010); 12(7): 518–527. 11-Shetti. A, Gupta. I, et al. Oral Candidiasis: