Manjinder Nanrey 12855110
To release RNAPII from pausing from the promoter proximal hairpin loop requires the recruitment of proteins such at TAT and P-TEFb. Currently supporting, but oversimplified, models tell us that Tat bind to the hairpin loop created by the Tar sequence of the HIV LTR, which then recruits P-TEFb. Through the use of CDK9, p-TEFb then phosphorylates Ser2 of the CTD, and allow for elongation. Nevertheless, it has been found that another protein called TCERG1 is involved in Ser 2 phosphorylation. This protein, originally called CA150 (2), has been characterized in the past, but there hasn’t been an appropriate model available that describes how TCERG1 works in vivo with regard to HIV-1 transcriptional elongation. In our current paper we have evaluated the role of TCERG1 and hypothesis that TCERG1 is a cofactor for HIV transcriptional elongation. We looked at the effects of TCERG1 knockdown by shRNA in PBL and Jerkat cells transfected with the HIV-1 LTR with the Luciferase reporter fused on the env gene, and found that both basal and Tat-activated transcription were diminished. This suggested TCERG1 served as a positive regulator of transcription. To test TCERG1’s effect on elongation we measured the amount of transcript at the distal regions of the LTR in HEK293T cells with a deletion of the Reverse transcriptase of HIV-1 transiently transfected plasmid. We found that TCERG1 knock down decreased transcript production by 30-40%. To directly measure the transcription rate we measured the level of transcript from the using 2 lines, one of which did not contain the shRNA for TCERG1 (control). We treated both cell lines with DRM, which block gene transcription in vivo, and measured the recovery of the distal tran...
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...peckles and Assembles Transcription and Splicing Components into Complexes through Its Amino and Carboxyl Regions." Molecular and Cellular Biology 26.13 (2006): 4998-5014. Molecular and Cellular Biology. Web. .
(4,7) Kodama, Yutaka, and Chang-Deng Hu. "Bimolecular Fluorescence Complementation (BiFC): A 5-year Update and Future Perspectives." BioTechniques 53.5 (2012): 285-98. BioTechniques. Web. .
(8) Shilatifard, Ali, et al. "Ell2, a New Member of an Ell Family of Rna Polymerase Ii Elongation Factors." Proceedings of the National Academy of Sciences 94.8 (1997): 3639-43. Print.
(5,6) Gaj, T., C. A. Gersbach, and C. F. Barbas. "Zfn, Talen, and Crispr/Cas-Based Methods for Genome Engineering." Trends Biotechnol 31.7 (2013): 397-405. Print.
"The Species of the Secondary Protein Structure. Virtual Chembook - Elmhurst College. Retrieved July 25, 2008, from http://www.cd http://www.elmhurst.edu/chm/vchembook/566secprotein.html Silk Road Foundation. n.d. - n.d. - n.d.
Miller, Kenneth R. and Joseph S. Levine. “Chapter 12: DNA and RNA.” Biology. Upper Saddle River: Pearson Education, Inc., 2002. Print.
In the novel, Hamid also explores Changez’s relationship with Erica as a metaphor of the challenges faced by South Asian Americans in their efforts to assimilate and “become” American through one, the morning after Changez came down for breakfast, he and Erica were the only two there and so they had a conversation. In their conversation, Erica asked him about his home life in Pakistan, to which Changez replied,
In humans and other eukaryotes, there is an extra step. RNA polymerase can attach to the promoter only with the help of proteins called basal (general) transcription factors. They are part of the cell's core transcription toolkit, needed for the transcription of any
Jena, Anupam B., Seth Seabury, Darius Lakdawalla, and Amitabh Chandra. "Abstract." National Center for Biotechnology Information. U.S. National Library of Medicine, 18 Aug. 2011. Web. 01 May 2014.
Gene therapy is a relatively new concept owing mainly to our current knowledge of the human body and the relatively modern understanding of genetic coding and process. We now are able to better identify and understand the genetic causes of human ailments, and are just beginning to understand how to fix, replace, or eradicate the chromosomal basis for these issues; this is the concept of gene therapy. However logistically dealing with the small structure of genes, chromosomes, and DNA is not as easy as repairing a cracked wall or damaged water pipe, we are dealing with complex and microscopic materials that ordinary tools cannot deal with. Manufacturing such tools to deliver corrective DNA into affected cells within the body is just one of the obstacles that scientists and researchers are facing.
... starts relaxing the supercoils and altering of DNA and interacts with DNA helicase SGS1 and plays a role in DNA recombination, also cellular aging and maintenance of genome stability. Alternate splicing results in multiple transcript variants. Additional spliced variants of the gene have been described, but their complete length is unknown.
"Virus-Host Cell Interactions during Hepatitis C Virus RNA Replication: Impact of Polyprotein Expression on the Cellular Transcriptome and Cell Cycle Association with Viral RNA Synthesis -- Scholle Et Al. 78 (3): 1513 --." The Journal of Virology. Web. 24 Mar. 2010. .
Trafton, A. (2013, June 23). MIT News. Enhancing RNA Interference, pp. 1-2. Retrieved 12 16, 2013, from http://web.mit.edu/newsoffice/2013/enhancing-rna-interference-0623.html
Li, Julang. "Mechanisms Involved in Targeted Gene Replacement in Mammalian Cells." Genetics. Vol. 156, 809-821. Oct. 2000.
Pardee, Keith, Alexander A. Green, Tom Ferrante, D. Ewen Cameron, Ajay DaleyKeyser, Peng Yin, and James J. Collins. "Paper-Based Synthetic Gene Networks." Cell 159.3 (2014). Science Direct. Cell Press. Web. 5 Nov. 2014.
Citation: Philips, T. (2008) Regulation of Transcription and gene expression in Eukaryotes. Nature Education 1(1)
Ottilingam, S. (1927, June 5). Tom O'Bedlam. National Center for Biotechnology Information. Retrieved March 29, 2014, from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2900000/
Research from the University of California San Diego Scripps Institution of Oceanography said that species in the ocean consume a projected 12,000 to 24,000 tons of plastic every year in the Pacific Ocean (Nall, 2014). Pollution of recyclable materials in the oceans is one of the leading causes of why some marine species are nearing extinction. Many authors of articles and books analyzing this topic tend to agree that pollution of our oceans is a problem. The future of this problem is where their ideas tend to differ. The following four literature reviews attempt to demonstrate and support my belief that pollution is getting worse in the ocean and more marine life ecosystems are being affected, but there are things that we as humans can do to change this. Imagine a world where we didn’t have to constantly worry about the vicious cycle of humans affecting animals and then animals in turn affecting us through consumption.
Grinde, Bjørn, and Grete Grindal Patil. "Abstract." National Center for Biotechnology Information. U.S. National Library of Medicine, 31 Aug. 2009. Web. 11 Apr. 2014.