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Investigation of water potential
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The Effect of Sucrose Solutions on Water Potential
Ronak P. Patel
South Western High School
The Effect of Sucrose Solutions on Water Potential
Results
Table 1: Raw Data for 3 Trials (Initial Weight and Final Weight)
Cups
Cup 1
Cup 2
Cup 3
Cup 4
Cup 5
Cup 6
Cup 7
Solution
Solution 1
Solution 2
Solution 3
Solution 4
Solution 5
Solution 6
Water
Cell
Potato
Potato
Potato
Potato
Potato
Potato
Potato
Initial
Weight (T1)
1.31g
0.79g
0.84g
0.90g
0.63g
1.00g
0.62g
Final Weight (T1)
1.28g
0.80g
0.85g
0.97g
0.71g
1.01g
0.74g
% change (T1)
-2.29%
1.27%
1.19%
7.78%
12.70%
1.00%
19.35%
Initial Weight (T2)
1.12g
0.69g
0.63g
0.49g
0.86g
0.99g
0.72g
Final Weight (T2)
1.08g
0.79g
0.63g
0.56g
0.90g
1.02g
0.80g
% Change (T2)
-4.00%
…show more content…
Table 2 shows the average percent change from the three trials. The average percentages were calculated by adding the percentages from the three trials for each cup and then dividing by three which was the number of trials. The calculations used to calculate water potential for each of the solutions are shown. The formula for solute potential used was -iCRT. Figure 1 shows the graph of the different sucrose solutions in relation to the water potential of those solutions. …show more content…
My hypothesis in this lab was that as the concentration of the sucrose solution increased, the water potential will decrease. I feel confident in the results because I feel that the procedure went according to as planned. To ensure validity of the final results, there were three trials completed. In addition to that, there was precision and accuracy taken into computing the calculations needed in this lab such as the water potential, solute potential, percentages and ultimately the the average percentages from the three trials. The lab could have been more refined if there was more time given to complete this. Time would have given more accurate results. In addition, the results would also be precise. As previously mentioned, sources of error could have been from collecting the data or a mix up with the three trials that were completed. Measuring probably could not have impacted the results because the weight was taken from an electronic scale that was properly “zeroed out” each time before a “cell” was measured. This lab could be modified to measure the weight of the solution before and after the interaction with the cell model. This would allow the observations of whether the cell took in solution or not. A weakness in this experiment is the use of only one type of “cell” and this can be easily improved by the addition of other cell models to represent a cell interacting with its surrounding
The mixture was poured through a weight filter paper and Sucrose washed with a 5ml of dichloromethane. The resulting solid was left in a breaker to dry for one week, to be measured. Left it in the drawer to dry out for a week and weighted it to find the sucrose amount recovered amount.
We then took 1ml of the 0.1% solution from test tube 2 using the glucose pipette and added it to test tube 3, we then used the H2O pipette and added 9ml of H2O into test tube 3 creating 10ml of 0.01% solution.
the replicate shows the same trend as the first experiment. I used a measuring cylinder and a beaker to measure out the amounts of water; however these did not seem to affect the quality of my results. To increase the accuracy of my results I could have perhaps used a burette. Even though I did the best I could to keep the experiment accurate, I did. some places there were mistakes that unintentionally occurred.
When the cell has all the water it can take inside of it the osmosis
Investigation of the Concentration and the Effect of Sucrose on Osmosis in Apple and Potato Tissues
Osmosis Experiment Planning Aim: The main subject that I will be planning to investigate is the effects of a concentrated sucrose solution on potato cells on the basis of the Osmosis theory. Background knowledge: The plant cell and its structure To understand osmosis in detail I will need to explain the plant cell (which is the cell included in the osmosis experiment) and its cell membrane. Below I have a diagram of a plant cell: [IMAGE] Osmosis is about the movement of particles from a higher concentrated solution to a lower concentrated solution to create an ethical balance via a partially or semi permeable cell membrane. Osmosis in simple terms is the exchange of particles between the cytoplasm inside the cell and the solution outside the cell. What makes this exhange is the partially permable cell membrane.
Osmosis in Potato Tubes Osmosis: Osmosis is the movement of water molecules through a semi-permeable membrane from a high concentration to a low concentration. Diagram: [IMAGE] [IMAGE] Aim: To see the effects of different concentration of sugar solution on Osmosis in potato tubes. Key factor: In the investigation we change the sugar solution from: 0%-10%-20%-30%-40%-50% this is the independent variable; the dependant variable is the change in mass. Prediction: I predict that all the potato tubes in pure water or low concentration sugar solution will swell because water enters their cells by osmosis.
molecules go in and out of the cell. There is no net movement of water
According to osmosis theory as the concentration of the sucrose solution increases the particles water potential increases and becomes higher than the particles that are in the
At point A the graph shows that no change in mass, of the potato, would have have occurred had we used a 0.2 (m) sucrose solution. This suggests that the concentration of water inside the potato would have been equal to the solution outside the potato. At point B (plain water), there is no indication that the cell is increasing in mass. This is because the cell is fully turgid and no more water can enter.
Investigating the Effect of Sugar Solution on the Weight and Size of Potato Cells Aim: To investigate whether the different concentration of sugar solution will affect the weight and size of the potato cells. General background information: Osmosis is defined as the movement of water or any other solution's molecules from an area in which they are highly concentrated to a region in which they are less concentrated. This movement must take place across a partially permeable membrane such as a cell wall, which lets smaller molecules (E.g. water) through but does not allow larger solute molecules to pass through. The molecules will continue to diffuse until the area in which the molecules are found to reach a state of equilibrium, meaning that the molecules are equally distributed throughout the cell, with no area having a higher or lower concentration than any other hence equal. Hypothesis/prediction: For this particular investigation I believe that the lower the concentration of the sugar solution in the test tube the mass of the potato will be greater and the longer the potato cells will be.
The mixture for that table’s flask was 15 mL Sucrose, 10 mL of RO water and 10 mL of Yeast, which the flask was then placed in an incubator at 37 degrees Celsius. In my hypothesis for comparison #4 the measurements would go up again with every 15 min. intervals because of the high tempeture and also be higher that then Controlled Table’s measurements. Hypothesis was right for the first part but was wrong for the second part of the comparison, the measurements did increase in the table’s personal flask but the measurements did not get higher than the Controlled Table’s measurements, see chart below. In conclusion, I feel that the substitution of glucose for sucrose made the enzymes work just as hard as the Controlled Table’s flask but just not as much because sucrose was too strong for the enzymes to
In conclusion, the method seems flawed in accurately measuring the glucose concentration of solutions. To make the experiment a "fair test," three main variables needed to be kept constant. Firstly, the volumes, including all measurements, i.e., the amount of Benedict's solution used and the amount of glucose water solution in each test tube. Secondly, the time that all the test tubes were kept in the water bath had to be the same for all test tubes, including solution X.
Through the osmosis experiment, it was clear that water moved into the cell due to a lack of water and an excess of solute; in the environment, there was an excess of water and a lack of solute. The biochemical testing relayed information about what minerals, particles, elements, or
There is also the potential of human error within this experiment for example finding the meniscus is important to get an accurate amount using the graduated pipettes and burettes. There is a possibility that at one point in the experiment a chemical was measured inaccurately affecting the results. To resolve this, the experiment should have been repeated three times.