Sucrose Concentration Lab Report

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The Effect of Sucrose Concentrations (0.00 to 0.73 mol/L) on the Germination Rates of Raphanus sativus seeds.
Introduction:
Research Question: What impact does different sucrose concentrations (0.00 to 0.73 mol/L) have on germination and subsequent growth of Raphanus sativus seeds?
Background information: Germination experiments are typically completed in many high school science classes; such as in the prerequisite to IB biology, where students analyze the effect of salinity or radiation on germination rates. Many scholarly publications have been published testing the effect of salts on plant germination, yet there are very few that have been produced on the effect of sucrose. In the few experiments conducted testing the impact of sucrose, …show more content…

If the seed is germinated, measure the radicle and plumule using a ruler measuring in centimeters.
Safety, Environmental, and Ethical Concerns:
When preparing solutions, wear safety goggles to prevent sucrose concentrations from contaminating eyes. Also use precaution when handling the glassware, as broken glass can cause serious injury. After the experiment, sucrose concentrations can be disposed of into normal water treatment systems because the sucrose is a natural, non-toxic substance. Make sure radishes are properly disposed off once the experiment is completed. This experiment has no ethical concerns because there were no risk of disturbing a natural ecosystem because the experiment was conducted in a laboratory nor any animals used in this process.
The images below represent different steps of the experiment, beginning on the left with image 1, center is image 2, and the right is image 3. Image 1 Image …show more content…

Surprising, a significant increase in the molarity was required to see a difference in the measured dependent variable. Because the sucrose concentration changed the molarity of the solution, the data showed that the germination rate decreased when the molarity increased. The increase in molarity also had correlated shorter root and shoot lengths in the seeds that germinated. An ANOVA test was conducted to determine whether there is a significant difference in the data, similar to a combination of T-tests. In the ANOVA tests, the F-value for both the mean shoots and roots was larger than the critical F-value. This response suggests that there was a statistical difference between the control groups and the experimental group, which means the null hypothesis was rejected and the alternative supported. The line of regression allows for a visual representation to affirm the correlated decrease as the molarity increases. After conducting the ANOVA test and running regression lines through the data collected, there was a statistical difference between the control group and the experimental group. However, the data suggests that there is a decrease in the percent germinated, as well as a decrease in the mean root and mean shoot length, as an increase in sucrose concentrations

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