Quality By Design Case Study

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1.2.3.7. Quality by Design
As PharmaKonic Ltd utilises Quality by Design for the process development and scale up studies executed in the previous Upstream Process to maintain process continuity, this concept will be integrated into the production of the Quality Strategy and Quality Maintenance System.
Quality by Design focuses on a risk based approach to ensuring process and product quality through an in depth understanding of product attributes and the overall process and product performance. This concept will be achieved by defining the product to be produced and through the identification of Critical Quality Attributes (CQA) and their potential impact upon the process and the final product. The Quality by Design System will be shown in the following diagram:

Figure 1.1: Quality by Design System adapted from (Huynh-Ba, 2009)
The Quality by Design concept is created based on process stages which are included as follows:
• Product designed based on requirements of consumers
• Process design consistently meets product Critical Quality Attributes (CQA)
• Quality system used to integrate process and product knowledge gathered during development
• Full understanding of potential impact of formulation components and process parameters on the quality of the product
• Evaluating and controlling causes of process variability
• Ensuring reliable and consistent quality throughout the process lifecycle by monitoring and integrating process improvement.

1.3. Quality Risk Management
Quality Risk Management has been considered a valuable element of an efficient quality system and once executed plays a crucial role in the assessment and control of the potential risks to drug quality throughout the entire manufacturing proce...

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... backbone, within this framework there is one O-linked carbohydrate which plays no significant role in maintaining bioactivity, three N-glycans attached show great influence over the stability, immunogenicity and overall solubility.(Li and d’Anjou, 2009) The amino acid sequence will be analysed for the correct structure through use of HPLC to differentiate between the 165 amino acids present within Epoetin omicron.
3. Isoelectric point
Epoetin omicron has been determined to have an isoelectric point of 3.5 to 5 and will be analysed to ensure this result by use of an isoelectric focusing gel. This will establish whether the in process sample analyzed shows any potential for protein modification such as glycosylation or phosphorylation and following this run will be measured for the correct isoelectric point by use of 2D gel electrophoresis. (LifeTechnologies, 2014)

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