Northern Leopard Frog Lab Report

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In the northern leopard frog, there has been experiments conducted regarding eye retraction when swallowing. It has long been held as an assumption that eye retraction may help the frog in pushing the food through the digestive tract. From observing northern leopard frogs from eating 1.5cm long crickets, it shows a high amount of variability as both the eyes and muscular area of the throat are seemed to have make physical contact with the food item. (e.g. Dickerson, 1906 Regal and Gans, 1976 Nishikawa, 2000 Schwenk). In order to conduct this experiment, male northern leopard frogs that have a snout length of 5.5cm to 7.0cm were acquired through commercial purposes. (Scherber, 1782). They were housed according to specific temperature specifications …show more content…

For this experiment, Rana temporaria were utilized since they have two midbrain nuclei that receive impulses since the neurons respond to stimulation of optokinetic pathways. (Cochran). Each of these neurons plays a different role in response types. Examples of such movements in neurons include both upward and downward moving targets from optokinetic perspective. Cells in a specific region have this sensitivity, and can even utilize their stimulation for increasing and decreasing rest rate. Oculomotor neurons were studied because they possess an exclusive upward or downward motion which would make the controls easier to identify and carry out the experiment. (Precht, 1982). Temporal nasal pattern movements are an additional type of neuron that have similar velocity preferences as simple sensory nuclei. To investigate the connectivity between motor and sensory nuclei, the region that they were derived from within the frog was restricted to the pretectum and abducens. A single connection between these areas is suggested through onset latency in antidromically motor neurons, activation of the pretectal cells, horseradish injections which involve labeling the axons, and horseradish injections into the region of abducens which create labeling of cells in pretectal region. (Gaupp, 1896). In this experiment, a length of time of two years and …show more content…

The mouse eyes were significant in that they provided results that can be compared with the data obtained from the frogs. These frog and mouse eyes were studied using X-ray diffraction. The changes regarding light induced reflections were recorded at 0.1seconds and 1.0 seconds in frog and mouse cells. (Chabre and Cavaggioni, 1973). The diffraction of disk membranes was measured to the 10th order to maintain specificity and detailed differences between mammalian and amphibian rod outer segments. For the experiment, a bullfrog ( Rana catesbeiana) eye was used and the frogs were in an environment that provided 12 hours of light and 12 hours of dark at a constant temperature of 8 degrees celsius for one to four weeks. A frog from the group was killed and the head was cut along the middle so that both eyes could be further studied. For the mouse eyes, BALB/C mice also maintained under the same conditions as the frogs were used. However, the eyes from the mice were removed from the sockets by cutting the optical nerve and tissues whereas the eyes on the frog remained intact. A similar X-ray analysis was used for both specimen and involved a beam passing from the back of the eye to the side where the light illuminates the eye from the front. (Corless 1972). A

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