Making a Wet Mount Slide
Purpose: The purpose of this lab experiment is to learn how to make a wet mount slide and observe it under a microscope.
Materials: The materials used in the lab experiment were…
1. Microscope
2. Microscope slide
3. Coverslip
4. Newspaper
5. Scissors
6. Medicine dropper
7. Water
8. Forceps
Procedure: The procedure followed in this experiment were…
1. Cut a small "d" from the newspaper and place it in the center of a clean microscope slide so that it is in normal reading position.
2. To make a wet-mount slide use the eye dropper and place a small drop of water over the specimen "letter "d") to be observed.
3. Place one slide of a clean coverslip at the edge of the drop of water at a 45-degree angle. Using the forceps carefully lower the coverslip over the letter "d" and drop of water. Try not to trap air bubbles under the coverslip, since these will interfere with you view of the specimen.
4. In figure 2 draw a picture of the letter "d" seen on the slide.
5. Clip the slide into place on the stage of the microscope and position it so that the letter "d" is directly over the center of the stage opening.
6. Look at the microscope from the side and use the coarse adjustment knob to lower the body tube until the low- power objective lens almost touches the slide.
7. Looking through the eyepiece use the coarse adjustment knob to raise the body tube until the specimen comes into view.
8. Turning no more than ¼ of a turn, use the fine adjustment knob to focus the letter clearly.
9. In plate 1, draw a picture of the letter "d" as viewed through the microscope. Note the magnification.
10. While looking through the eyepiece, move the slide to the left. Notice which way the letter seems to move. Now move the slide to the right. Again notice which way the letter seems to moves.
11. To switch to the high- power objective lens, look at the microscope from the side. Revolve the nosepiece so that the high-power objective lens clicks into place. Using the fine adjustment knob only, bring the specimen into focus.
12. In plate 2 draw a picture of the letter "d" as seen with the high-power objective lens. Note the magnification.
13. To switch to the medium-power objective lens look from the side and revolve the nosepiece so that medium-power objective lens clicks into place. Using the fine adjustment knob only, bring the specimen into focus.
14. In plate 3 draw a picture of the letter "d" as seen with the medium-power objective lens.
Put the slide under the microscope and focus it so that you can clearly see the individual heartbeats.
Using the scopula, take a small amount of the substance and add it to the spot plate. Add deionized water to the section with the substance. Stir to see if the substance dissolves or not. Record your observations.
1. Using a dropper, place few drops of the unknown substance on the tissue paper.
6. Place the test tube in the beaker. Secure the test tube and thermometer to the retort stand using clamps. Begin heating the water bath gently.
To identify the bacteria under the microscope was not easy since it was our first time identifying bacteria. After one of our trials we would pour a drop of water on to a slide, and then add Iodine Sodium over that because the Iodine helps the bacteria to show more. We would then observe the water and record our qualitative observations, which were ½ from the microscope observation and the other ½ was while watching the water...
For an eye to focus correctly on an object, it must be placed in a certain position in front of the eye. The primary focal point is the point along the optical axis where an object can be placed for parallel rays to come from the lens. The secondary focal point is the point along the optical axis where in coming parallel rays are brought into focus. The primary focal point has the object's image at infinity, where as the secondary focal point has the object at infinity. For people who have myopic eyes, the secondary focal point is anterior to the retina in the vitreous. Thus, the object must be moved forward from infinity, in order to be focused on the retina. The far point is determined by the object's distance where light rays focus on the retina while the eye is not accommodating. The far point in the myopic eye is between the cornea and infinity. The near point is determined by which an object will be in focus on the retina when the eye is accommodating. Thus, moving an object closer will cause the perception of the object to blur. The measurement of these refractive errors are in standard units called diopters (D). A diopter is the reciprocal of a distance of the far point in meters (Vander & Gault, 1998). The myopic condition manipulates these variables in order to ultimately make a nearsighted individual.
n.d. - n.d. - n.d. Retrieved from http://www.dd-692.com/atomic.htm.
As it became my hobby to study quite a few microscopic and gross preparations for hours every day. Working under a fine supervision of my pathology professor Dr. Bekhtereva, made me aware of my ability to identify and follow a specific pattern in a slide. My mentor emphasized how important it is to be able to combine this innate visual ability with rigorous scientific
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For my preliminary work, I used a 50 mm piece of potato. It was easy
Create wells: put a comb template in the middle of the tray; wait until the mixture becomes solid. After, remove the comb standing straight. 4. Remove rubber ends: transfer the gel tray into the horizontal electrophoresis and fill it with the concentrated electrophoresis buffer. 5. Materials and methods: Experiment: 1st, prepared milk samples should be already done by the teacher.
First we placed the slide under the simple microscope and observed it at ten times magnification level. We each took turns looking. We then copied them as drawings into our Cornell notebooks. After that we changed the magnification to fifty times and observed the slide. We each took turns looking. We then copied what we saw into our Cornell notebooks. After that we changed the magnification level to sixty times and we each took turns looking at the slide. We then copied what we saw into our Cornell notebooks and sat down together. When we were all done drawing we spoke about what we seen and gave each other ideas on how to write our Lab Reports.