LCB

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For many years researchers have endeavoured to enhance current methods in cervical screening which utilises the Papanicolaou smear (also known as a Pap smear). Limitations in the smear have caused major emphasis to increase sensitivity and specificity whilst also creating an automated screening procedure. The Pap smear was initially introduced in the context of cervical screening in the detection of pre-cancerous lesions of the cervix in 1940’s (Koss, 1989). Cervical screens are vital as research has shown 90% of women with invasive cervical carcinoma could be prevented by finding any lesions early (Grace. et al. (2001). Cervical screening in the UK presented with an annual reduction of 7% but the Pap smear also possesses many limitations such as high error rates (Graff. et al. 1987) Figure 1.

Figure 1: Age Standardised Incidence and Mortality from Cervical Cancer, England (Cervical Screening Programme Review, 1999)
Rates per 100,000 per annum - directly age standardised using the European standard population.
Cervical cancer possesses a high incidence rate of cancers among women. The uterine cervical cancer can be assessed at the squamo-columnar junction and the transformational zone. Within this site there is continuous metaplastic changes, between the squamous epithelium of the ectocervix and the columnar epithelium of the endocervix.
Metaplastic changes mainly occurs at puberty and first pregnancy, therefore most cases are seen in women under 30. Every women can develop cervical cancer, however there are factors that can increase the risk such as infections like HPV or the use of oral contraceptives (Schiffman. 1995).
The Pap stain was designed upon the knowledge of tumour cells lose property which can be readily dislo...

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... of 25 showed to be less effective whilst the same was understood testing after 64 if there is no history of cancers.
Tests for cervical cancers and diseases are always progressing and the next step in identification molecular biomarkers. The use of these panels should coexist alongside the current LCB infrastructure increasing the chances of early diagnosis. HPV and its viral onco genes E6 and E7 has been found in 99+% of cervical neoplasia when both genes are expressed by damaging the DNA engineering dis-regulation (Walboomers. et al. 1999) (Giannoudis. et al. 2001). Other tumour molecules and proteins such as Ccd6 and p16(INNK4A) are over expressed in pre-cancer and malignant lesion production by un-restricted proliferation of malignant cells (Astbury, 2006). These markers could increase time periods between tests by increasing sensitivity (Hoyer. et al. 2005).

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