Enzyme Temperature Lab Report

Investigation to Determine the Effect of Temperature on the Activity of the Enzyme Amylase

I am trying to find out if changing temperatures affects the activity of enzymes. The Input variable I will test is temperature; the range I will use for this is 0-80°C. The out come variable to be measured is the speed of the reaction.

Hypothesis

I believe that at a higher temperature the enzyme will react quicker, but at a certain temperature the enzyme it will stop working.

I think this because enzymes work well at higher temperatures because particles have more energy resulting in more successful collisions between them and a quicker reaction. I also know that if the temperature is too high the enzyme

A graph I produced with the results is on the next page. As I increased the temperature the reaction took place faster and at my highest recorded temperature of 85°C the average reaction time was 6 seconds. At a higher temperature the starch particles move around with more energy resulting in more successful collisions meaning a faster reaction.

My conclusion didn’t support my prediction that the enzyme will become denatured and won’t work after the reaction has reached the optimum temperature but I believe something must have gone wrong.

Evaluation

I believe that some of my results were inaccurate because there was a misread with the temperatures or that we didn’t let the thermometer reach its final settling temperature and recorded the temperature too early. Also we might have started the reaction too early before the solution was at the correct temperature because the thermometer was in the beaker rather than the test tube and not measuring the