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Investigating factors affecting rate of reaction
What are some factors that influence the rate of reaction
Investigating factors affecting rate of reaction
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Investigation of the Effect of Temperature on the Rate of a Lipase Reaction
I am going to look at the effect of temperature on the rate of a
Lipase Reaction, and to look at this I will be changing the
temperature variable. In order to measure this, I will be doing the
following practical:
1) Add 5cm³ of milk and 1cm³ of Lipase solution at different
temperatures.
2) Then I will measure the reaction every 30 seconds, from 30 seconds
to 2 minutes with a pH probe (as lipase breaks down into fatty acids)
to test the strength of the acid produced. This will show how well the
enzyme has worked and a pattern should emerge in my results relating
the temperature to the strength of the acid. The stronger the acid,
the better the enzyme has worked. This will be shown in my results as
the more under a pH of 7 the final pH is, the better the enzyme has
worked during the reaction at a certain temperature. For example, a
final pH of 6.1 and a final pH of 7.3 shows that the first pH is
stronger and therefore more acid has been produced as the lipase have
broken down into fatty acids.
I will be using a range of temperatures at which to add the milk and
lipase. This should ensure that I get a fair result as I am using a
range of high and low temperatures from which to compare. I will get
readings at 20, 30, 40, 50 and 60°C from which a trend should show. I
shall repeat the experiment 3 times, and then take average results to
ensure the most reliable results possible.
Temperature (°C)
Lipase Amount (cm³)
Milk Amount (cm³)
20
1
5
30
1
5
40
1
5
50
1
5
60
1
5
I will use a pH probe to test the pH of the acid produced.
One thing that needs to be kept constant is the amount of water for each temperature. If the amount of water is varied that means that the data would not be that reliable and easy to look at. This can be kept constant by measuring how much water by looking at the meniscus. Another constant has to be the Alka-Seltzer tablets. This has to be kept constant because there are many different Alka-Seltzer tablets like there are ones that are citrus flavored and there are ones that treat your flu.
The effect of a change in PH on enzymes is the alteration in the ionic
Using a Bunsen burner, tripod and beaker of water 100 degrees could also be tested and 0 degrees was tested by using ice. (I didn’t investigate the 80 degrees temperature). Fair test: Below is a list of things that were kept the same throughout the investigation: Volumes of lipase and milk (by using syringes); volumes of phenolphthalein and sodium carbonate (using pipettes); (best volumes from the preliminary work were used). Each temperature was repeated three times to get a good average. The milk and lipase were equilibrated to the right temperatures before the lipase was added to the milk.
1. Put 400 mL of tap water into a beaker. Heat it on a hot plate until it starts to boil.
4. Pour hot water into one beaker and adjust the temperature to 39°C by adding colder water if needed
or it may be optimum it depends on what is the best pH that the enzyme
enzyme. As I'm sure you are well aware, enzymes do not get used up in
I decided to experiment with pHs within the range pH 2 to pH7, as I
The Effect of Temperature on the Rate of Reaction Between Hydrochloric Acid and Calcium Carbonate
Investigation of How Temperature Affects the Rate of Reaction of Sodium Thiosulphate and Hydrochloric Acid
- Temperature was measured after and exact time i.e. 1 minute, 2 minutes, 3 minutes.
From looking at the results I can conclude that when the pH was 3 and 5. No oxygen was produced, therefore no reactions were taking place. This was because the pH had a high hydrogen ion content, which caused the breaking of the ionic bonds that hold the tertiary structure of the enzyme in place of the syringe. The enzyme lost its functional shape.
Before conducting my experiment I will research into, amongst other things, the factors that affect the rate of a reaction. This is so that I may enough information to understand the effect of temperature on the rate of a reaction and also gain appropriate understanding to make a suitable prediction as to what the outcome of my experiment will be.
The aim of my investigation is to find out whether the increase of temperature increases the rate of reaction between the two reactants of Sodium Thiosulphate and Hydrochloric acid. I will then find out and evaluate on how temperature affects this particular reaction. Factors There are four main factors, which affect the rate of reaction that are considered as variables for the experiment I will be doing, they are the following: Molecules can only collide when two of them meet together.
The pH of the solution would alter the rate of the reaction if it was