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Investigating factors affecting rate of reaction
What are some factors that influence the rate of reaction
Investigating factors affecting rate of reaction
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Investigation of the Effect of Temperature on the Rate of a Lipase Reaction I am going to look at the effect of temperature on the rate of a Lipase Reaction, and to look at this I will be changing the temperature variable. In order to measure this, I will be doing the following practical: 1) Add 5cm³ of milk and 1cm³ of Lipase solution at different temperatures. 2) Then I will measure the reaction every 30 seconds, from 30 seconds to 2 minutes with a pH probe (as lipase breaks down into fatty acids) to test the strength of the acid produced. This will show how well the enzyme has worked and a pattern should emerge in my results relating the temperature to the strength of the acid. The stronger the acid, the better the enzyme has worked. This will be shown in my results as the more under a pH of 7 the final pH is, the better the enzyme has worked during the reaction at a certain temperature. For example, a final pH of 6.1 and a final pH of 7.3 shows that the first pH is stronger and therefore more acid has been produced as the lipase have broken down into fatty acids. I will be using a range of temperatures at which to add the milk and lipase. This should ensure that I get a fair result as I am using a range of high and low temperatures from which to compare. I will get readings at 20, 30, 40, 50 and 60°C from which a trend should show. I shall repeat the experiment 3 times, and then take average results to ensure the most reliable results possible. Temperature (°C) Lipase Amount (cm³) Milk Amount (cm³) 20 1 5 30 1 5 40 1 5 50 1 5 60 1 5 I will use a pH probe to test the pH of the acid produced.
1. Put 400 mL of tap water into a beaker. Heat it on a hot plate until it starts to boil.
4. Pour hot water into one beaker and adjust the temperature to 39°C by adding colder water if needed
Using a Bunsen burner, tripod and beaker of water 100 degrees could also be tested and 0 degrees was tested by using ice. (I didn’t investigate the 80 degrees temperature). Fair test: Below is a list of things that were kept the same throughout the investigation: Volumes of lipase and milk (by using syringes); volumes of phenolphthalein and sodium carbonate (using pipettes); (best volumes from the preliminary work were used). Each temperature was repeated three times to get a good average. The milk and lipase were equilibrated to the right temperatures before the lipase was added to the milk.
enzyme. As I'm sure you are well aware, enzymes do not get used up in
Over the observed fifty seconds, there was a consistency among the temperatures. Without a calculated percent error, we are able to assume the average temperature was twenty-six degrees Celsius. There are factors that could have caused error to arise in our data collection. One factor could be that the temperature of the room was not consistent throughout the room. Another factor may have been the performance of the thermometer. The grasp in which the thermometer was held for procedure B may also be a factor.
Investigation of How Temperature Affects the Rate of Reaction of Sodium Thiosulphate and Hydrochloric Acid
My aim is to see the effects of a change in temperature on the rate of
be to increase the temperature by only 5 C each time instead of 15 C.
From looking at the results I can conclude that when the pH was 3 and
or it may be optimum it depends on what is the best pH that the enzyme
The effect of a change in PH on enzymes is the alteration in the ionic
I decided to experiment with pHs within the range pH 2 to pH7, as I
The pH of the solution would alter the rate of the reaction if it was
The Effect of Temperature on the Rate of Reaction Between Hydrochloric Acid and Calcium Carbonate
Before conducting my experiment I will research into, amongst other things, the factors that affect the rate of a reaction. This is so that I may enough information to understand the effect of temperature on the rate of a reaction and also gain appropriate understanding to make a suitable prediction as to what the outcome of my experiment will be.