What pH the Enzyme Named Peroxidase Works Best At

What pH the Enzyme Named Peroxidase Works Best At

The aim of my investigation is to find out what pH the enzyme named

peroxidase works best at. We can find the enzyme peroxidase if we

grind up celery, or most other animal and plants. A chemical reaction

in plants and animals produce hydrogen peroxide, this can poison them

if it is allowed to accumulate. The enzyme peroxidase acts as a

catalyst for decomposing hydrogen peroxide, it is broken down into

water and oxygen. The reaction is:

2H20 = 2H20 + O2

(Aq) (L) (G)

I plan to measure the amount of oxygen given off by the celery at

different pHs and whichever pH helps the enzyme produce the most

oxygen. Before I do this I must do a preliminary plan to see what my

other variables should be so that I can get the best results.

I predict that the optimum pH will be around 7 because if it

goes any lower or higher than this it could change the enzymes shape

and/or charge properties of the substrate so that either the substrate

cannot bind to the active site or it cannot undergo catalysis.

Different enzymes have different optimum pHs and it is hard to say

what it will be because some enzymes work best at low temperatures and

others at high temperature. As you can see from the graph the two

different enzymes, represented by red and green lines, have a

different optimum pH. When the pH is altered is will become denatured

or it may be optimum it depends on what is the best pH that the enzyme

responds to.

I believe it will be 7 because this is in the middle, 7 isn’t too high

or too low but it could be wrong depending on the enzyme. I think it

is pH7 because this is ...

... middle of paper ...

...yme more. I would also try and

get the celery to be the same age because my experiments over two days

with two different celeries, which could have had an effect and gave

odd results. The odd results could of came from anything though, we

could of measured slightly wrong, measured the solutions wrong, or

when we did measure the solutions there could have been leftover acid

in the syringes. I was able to get sensible results in the end after

redoing the tests that had odd results. But overall I successfully

carried out my investigation fairly and accurately. My experiment was

good because I have measured all my solutions accurate, added the

solutions together in the same order, timed my experiment to the best

of my ability, I have kept the temperature the same and I have

produced an experiment that is fair and accurate.