Imtroduction-
Histology is the study of the details of the biological cells or tissues in a specimen prepared by histological technique and by using electron or light microscope.
It plays an important role in the fields of verternary,medicine,etc
The word histology is derived from the greek word where histo means tissue and logy means study.therefore histology is the study of the tissues.therefore the person who performs histology by a framed set of procedures is called as a histologist.
Histology is the scientific study of biological tissues.
Histology is done by taking thin sections of the tissue to be examined.to provide assistance and enhance the accuracy of the study various histological stains are used.
Procedure-
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24. Movat's Pentachrome Stain - can be used to study connective tissue.
25. Mucicarmine - a very specific Mucin Stain
- can be used to observe epithelial mucins
26. Mucin Stains - a category of histology stains generally used to indicate one or more types of mucopolysaccharide substances in tissues.
26. Myloperoxidase (MPO) - can help identify cytoplasmic granules characteristic of myeloid cells (i.e.leukocytes that are not lymphocytes).
- can be used to screen peripheral blood samples for indications of myelogenous leukemia, a cancer of the white bllod cells.
27. Nissl Stains - are basic dyes
- can be used to stain RNA and DNA
- can be used to see Nissl bodies (which are also known as "Nissl granules" and as "tigroid bodies") in neurons.
28. Nuclear Fast Red - can be used to stain cell nuclei red (the cytoplasm will be unstained or yellow)
29. Oil Red O - a fat satain
- can identify neutral lipids and fatty acids in smears and tissues
- fresh smears or cryostat sections of tissue are necessary because fixatives containing alcohols, or routine tissue processing with clearing, will remove
This is used because in certain situations, there may be too little blood present at a crime scene to be able to be seen. It can also help when a suspect has attempted to “clean up” the blood after committing the crime. In these cases, luminol can be used to see any evidence of blood that cannot be seen with the naked eye. The glow from the reaction only lasts for about thirty seconds and requires dark conditions to be able to see, but investigators are able to document it in photographs.
amounts and how to analyze the specimine using gel electrophoresis. The samples of DNA were
If a larger sample is required, we may make an incision in the skin (open biopsy) and remove a larger section of muscle.
The hyperplasia foci can be seen as early as 2 months, as depicted by the histological picture. The number of cells with hyperplasia is then
Moreover, I will let the patient become familiar with any instruments such as a speculum, and demonstrate the tools that will be used to obtain tissue samples that would be used during the examination. Explaining the procedure is also a significant step; this will allow the patient have a sense of control during the examination as we ask and answer questions about their current state, that would help disseminate any concern they may have. Meanwhile, they can learn about what may happen, related to any body sensations or feelings they can experience during the procedure, all while the provider continues to develop rapport and patient’s trust
...cap of the sperm pink and the nucleus red, and a picroindigocarmine dye, which turns the mid piece of the sperm blue and the tail of the sperm green. The stained samples would then be placed under a microscope and hopefully spermatozoa would be present so that DNA testing could be performed.
This form of testing is used for determining if an individual is inebriated or not. James and Nordby (2009) stated that an equilibrium exists between alcohol in the bloodstream and alcohol in the lung such that, on average, the concentration of blood alcohol is 2100 times greater than the concentration of breath alcohol (p. 63). Since this equilibrium exists, one can measure the breath alcohol and infer the corresponding alcohol concentration in the bloodstream (James & Nordby, 2009 p. 63). An advantage of this form of testing is that it can be conducted without drawing a blood sample and testing it. This allows personnel such as police officers to test individuals on the spot versus having to take someone in and draw a blood sample and wait for results to come
Osman, Tagwa. "Detection of Cytomorphological Changes in Oral Mucosa." NCBI. U.S. National Library of Medicine, 26 Sept. 2011. Web. 19 Nov. 2013.
Though our knowledge of the human body has developed drastically, there will always be more to learn, especially in the field of pathology. In addition to education, this procedure is also used to improve the quality of care that healthcare workers provide for their patients, especially in terms of undetermined diagnoses. Post mortem examinations can help to better identify pathologies, and allow a “hands on” approach that cannot be obtained readily.
the gain or loss of water when samples of the tissue are placed in a
my check off sheet with the hematology supervisor. One of the first things I learned was
Medical laboratory technologists, also known as histotechnologists, work in a laboratory environment diagnosing diseases, doing research, or instructing others. Histotechnologists detect tissue abnormalities and treatments for the diseases causing the abnormalities, a vital role in the allied health profession. Histotechnologists prepare very thin slices of human, animal, or plant tissue in order to examine it using a microscope. This is considered one of the most important parts of the complicated process of medical investigation used to establish and confirm a diagnosis.
There are three simple tissues namely, parenchyma, collenchyma, and sclerenchyma. Parenchyma is thought of as the ground tissue of an axis since it occurs in greatest abundance and is the tissue in which the vascular tissues are embedded. Parenchyma cells may be isodiametric in both the pith and the cortex, but are more mostly longitudinally elongated in the cortex. They have comparatively thin walls. Wall layers are continuously shaped regions. These simple pits usually occur opposite each other, forming pit-pairs in the walls of contiguous cells. Plasmodesmata (specialized strands of endoplasmic reticulum) form interconnections with the protoplasts of adjacent living cells through simple
In 1849, he completed his studies of cell growth and reproduction of their division into two cells. He concluded that every cell comes from an already existing cell. A prolific writer, his scientific writings alone crossed 2,000 in number. Among his books, “Cellular Pathology” was published in 1858 and is regarded as the root of modern pathology. This work also popularised the third dictum in cell theory: “All cells come from cells”. Virchow was the first to precisely describe and give names of diseases such as leukemia, chordoma, ochronosis, embolism, and thrombosis. He also coined scientific terms, chromatin, agenesis, parenchyma, osteoid, amyloid degeneration, and spina