H Influenzae Research Paper

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Most of the infections seen today are from H. influenzae and it is the most clinically relevant (lecture 1, slide 30).
H. influenzae can be responsible for many infections, such as; ear infections, sinusitis, bronchitis, pneumonia, conjunctivitis, meningitis, epiglottitis, cellulitis, septic arthritis, and many more. If the infection is not treated, it can be life threatening in small children and immunocompromised patients (lecture 1, slide 31).

H. influenzae has special growth requirement that need to be met. It rarely grows on SBA, does not grow on Mac, but likes to grow on chocolate agar. Chocolate (choc) agar is a good source of both NAD (factor V) and hemin (factor X) (Tille, chapter 32, pg 406). When choc agar is being made, the blood being used for it is heated up, releasing NAD from the hemolyzed blood cells and activating hemin ( Todor, K.). …show more content…

The gram stain shows many PMNs, moderate-small sized GNR, rare GPC, and no epithelial cells. The sample is now plated to SBA, Choc, and Mac agar plates, incubated in a CO2 incubator of 35-37’C over night.

I. Possible Haemophilus A. Gram stain = small GNR 1. Presumptive for Haemophilus sp.
II. Culture A. SBA, CHOC, Mac, Quad plate
III. Growth exam and testing A. No growth on SBA or Mac. Growth on choc plate.
1. Round and large, greyish/white and opaque, flat, slightly mucoid

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