Comparing Amount of Glucose in Orange Fruit, Grapefruit and Lemon Fruit
I have been given 4% of glucose solution, benedict's solution and
distilled water to find out the concentration of glucose of these
three juices; orange, lemon, and grapefruit.
The method of serial dilution has to be done to work out the
concentration; this is because the concentration of glucose is far too
concentrated. A dilution factor has to be worked out; I will be using
a dilution factor of 5, which means the amount of glucose and the
percentage of glucose will always divide by 2 as the dilution solution
carries on.
Theory
Orange, lemon and grapefruit are all fruits which contain
carbohydrates which include reducing sugars. Glucose is a hexose
sugar, which is a monosaccharide with six carbon atoms in each of its
molecules.
Benedict's solution is a deep-blue alkaline solution used to test for
the presence of the aldehyde functional group, CHO - which are present
in reducing sugars. Benedict's solution changes colour when a reducing
sugar is present because of a chemical reaction, which reduces the
Copper II sulphate (which is soluble) to copper I oxide, which is
insoluble and produces a precipitate. The benedict's solution changes
colour from blue to green, yellow, orange, brown and finally red - as
the amount of sugar increases.
Colorimeter
Instruments for the analysis of coloured solutions include the
colorimeter. In both these instruments, light emitted from a source
passes through the sample to be analysed, and the intensity of the
emergent beam is measured by a photoelectric device and recorded on a
milliameter. The wavelength...
... middle of paper ...
... 8.75
0.5
5
0.6 (0.625)
9.4 (9.375)
0.25
6
0.3 (0.3125)
9.7 (9.6875)
0.125
Grapefruit Juice
Test tube
Glucose ml
Distilled water ml
Glucose %
Colour Transmission
Glucose Concentration
1
10
-
4
2
5
5
2
3
2.5
7.5
1
4
1.25
8.75
0.5
5
0.6 (0.625)
9.4 (9.375)
0.25
6
0.3 (0.3125)
9.7 (9.6875)
0.125
Orange Juice
Test tube
Glucose ml
Distilled water ml
Glucose %
Colour Transmission
Glucose Concentration
1
10
-
4
2
5
5
2
3
2.5
7.5
1
4
1.25
8.75
0.5
5
0.6 (0.625)
9.4 (9.375)
0.25
Briefly describe an alternative technique that could be used to measure the amount of glucose within sports drinks. (5 points)
2. A test tube was then filled with 35ml of yeast and placed in the
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