2. Computation Methods and Theory The Transient Analysis Tool has 2 fundamental components. (1) Preparation of raw data, and (2) transient analysis of the prepared data. With regard to the first component, there are four subcomponents. (i) Import data (ii) Cell separation (iii) Information marks (cell deletion and -background) and (iv) Transient separation. The second more substantive component can be subdivided into polynomial fitting of the baseline phase, deflection phase, peak phase and reflection phase and exponential fitting of the return phase. According parameters reflecting the kinetics of intracellular calcium concentrations and contractility are extracted from the fit results. 2.1 Preparation of Raw Data Preparation of raw data …show more content…
Text files can be obtained from Ionwizard (.ZPT files) via the export function. A detailed description of how text files can be exported correctly from IonWizard can be found in the User Guide (Attachment 2). The imported data are checked for the right composition by evaluating the (column) headers. The imported data is put into a data table with the according column headers classified as ‘Contractility Data’, ‘Calcium data’ or optional other data types. If the imported data do not match with any of the header compositions the data are not imported and the user is notified with an error dialog: ‘file column header is …show more content…
reversed extrema or reversed ‘greater than’- and ‘smaller than’ signs). The direction can be determined by using the baseline, the maximum- and the minimum value of a transient. The difference between the baseline and the maximum- and minimum value gives the maximal- and minimal deflection. As stated in Monotonic Transient Analysis-Defined (IonOptix), ’A positive-going transient is identified as having a greater maximal deflection than minimal deflection and a negative-going transient is identified as having a greater minimum deflection than maximum
In life, it is critical to understand what substances can permeate the cell membrane. This is important because the substances that are able to permeate the cell membrane can be necessary for the cell to function. Likewise, it is important to have a semi-permeable membrane in the cell due to the fact that it can help guard against harmful items that want to enter the cell. In addition, it is critical to understand how water moves through the cell through osmosis because if solute concentration is unregulated, net osmosis can occur outside or inside the cell, causing issues such as plasmolysis and cytolysis. The plasma membrane of a cell can be modeled various ways, but dialysis tubing is especially helpful to model what substances will diffuse or be transported out of a cell membrane. The experiment seeks to expose what substances would be permeable to the cell membrane through the use of dialysis tubing, starch, glucose, salt, and various solute indicators. However, before analyzing which of the solutes (starch, glucose, and salt) is likely to pass through the membrane, it is critical to understand how the dialysis tubing compares to the cell membrane.
A Transient Ischemic Attack, generally referred to as a TIA, is a type of stroke that only last a few min...
The above events end in cell death, including depletion of ATP, changes in ionic concentrations of sodium, potassium, and calcium, increased lactate, acidosis, accumulation of oxygen free radicals, intracellular accumulation of water, and activation of proteolytic processes.(Deb, Sharma, & Hassan, 2010). Surrounding this is the penumbra(Rodriguez-Yanez et al., 2006)
The experiment is aimed at giving a better understanding of the osmosis process and the different conditions in which osmosis occurs. INTRODUCTION When a cell membrane is said to be selectively permeable, it means that the cell membrane controls what substances pass in and out through the membrane. This characteristic of cell membranes plays a great role in passive transport. Passive transport is the movement of substances across the cell membrane without any input of energy by the cell.
approach to the issue, gains in one range may be offset by the setback in another.
Diffusion and osmosis in living cells are greatly effected by factors such as solution concentration, temperature, and surface area of the cell. This was studied using agar cubes of different sizes, dialysis tubing, and potato cores to demonstrate how the different factors affect cells. The process of cellular respiration can be altered due to temperature differences. Enzymes also greatly affect the molecular processes that occur. Enzymes are denatured at variety of pH and causes inactivity of the enzyme in the reactions.
The time taken for this to happen is the measure of the rate of reaction. We must do this several times, and change the concentration of sodium thiosulphate. The rate of reaction is a measure of the change, which happens during a reaction in a single unit of time. The things that affect the rate of reaction are as follows. Surface area of the reactants Concentration of the reactants
This assay was used to measure the concentration of protein in a solution. Use it to determine the concentration of our saved supernatants 1-6. The total amount of protein is determined and so is the phosphatase activity. By combining the Bradford reagent with our diluted supernatants in a test tube and letting sit for 5 minutes to be read at 595nm we were able to determine the mg/ml of protein. The absorbance was read on a spectrophotometer which measures the light intensity. Using a standard curve we were able to get our readings. The procedure was taking the six supernatants and aligning them to six test tubes. A solution was created with 50x dilution for sup1, 10x for sup 2-3, and no dilution for sup 6. A Bradford reagent was then added to each. Afterwards analyzed using the A595 wavelength. A formula was then used to determine the amount of active protein. Sup 1 yielded 104.31 mg/ml, sup 2 yielded 22.33 mg/ml, sup 3 yielded 1.21 mg/ml, and sup 6 yielded 0.66
The heart has a relatively long refractory period due to the plateau phase. The plateau phase occurs from the maintenance of a high calcium conductance and a delay in the subsequent increase in potassium conductance after a cardiac action potential. The heart membrane remains in a refractory or contracted state until the heart has fully returned to a relaxed state; therefore, tetanus or wave summation does not occur in cardiac muscles (Randall, 2002). When the frog’s heart was stimulated with single stimuli, an extrasystole and an increase in frequency was observed during the relaxation phase, but the amplitude remained constant. These results were analogous to the multiple stimuli treatment, which we expected. Because both single and multiple
the standard curve plotted Absorbance versus concentration for known amounts of ortho-nitrophenolate will be drawn. This will be useful in determining the rate of reaction for the Lactase enzyme in different conditions (amount of product/time = rate). D
The surge of increased intracellular Ca2+ levels will pass through the GJC to adjacent neighboring cells. This surge of Ca2+ is one trigger for proceeding towards apoptosis.
A lot of the weaknesses identified in the SWOT analysis could have a grave impact on academic studies. However, to try to change all of them whilst conducting academic studies may be too onerous, therefore the sensible approach would be to identify the three that would have the most effect on this programme’s studies and try to reduce this impact or mitigate it in its entirety. The three factors chosen based on importance are:
Since 1950's the electromagnetic geophysical methods have been used extensively and with great success to delineate conductive zones at depths. The aim of EM method responds to high conductivities rather than to contrasts in conductivity as is the case with the classical direct current (DC) method. This means that EM methods can detect small changes in conductivity in a conductive terrain by the correct selection of technique and array and secondly that resistive layers became transparent in contrast to the DC resistivity method where penetration of such layers is difficult. According to (Mares et al., 1984) the EM methods can be divided into three fundamental groups:
remain in equilibrium condition, their history is rhythmic as a result of the mechanisms of