Advantages Of Transient Analysis

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2. Computation Methods and Theory The Transient Analysis Tool has 2 fundamental components. (1) Preparation of raw data, and (2) transient analysis of the prepared data. With regard to the first component, there are four subcomponents. (i) Import data (ii) Cell separation (iii) Information marks (cell deletion and -background) and (iv) Transient separation. The second more substantive component can be subdivided into polynomial fitting of the baseline phase, deflection phase, peak phase and reflection phase and exponential fitting of the return phase. According parameters reflecting the kinetics of intracellular calcium concentrations and contractility are extracted from the fit results. 2.1 Preparation of Raw Data Preparation of raw data …show more content…

Text files can be obtained from Ionwizard (.ZPT files) via the export function. A detailed description of how text files can be exported correctly from IonWizard can be found in the User Guide (Attachment 2). The imported data are checked for the right composition by evaluating the (column) headers. The imported data is put into a data table with the according column headers classified as ‘Contractility Data’, ‘Calcium data’ or optional other data types. If the imported data do not match with any of the header compositions the data are not imported and the user is notified with an error dialog: ‘file column header is …show more content…

reversed extrema or reversed ‘greater than’- and ‘smaller than’ signs). The direction can be determined by using the baseline, the maximum- and the minimum value of a transient. The difference between the baseline and the maximum- and minimum value gives the maximal- and minimal deflection. As stated in Monotonic Transient Analysis-Defined (IonOptix), ’A positive-going transient is identified as having a greater maximal deflection than minimal deflection and a negative-going transient is identified as having a greater minimum deflection than maximum

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