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Biofilms microbiology
Biofilms microbiology
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Biofilms are defined as complex aggregates of microorganisms which are interlinked and secrete extracellular slime, which forms the matrix for the films. The extracellular slime is chiefly made up of polysaccharides. Biofilms are usually irreversibly attached to a surface, in that once a biofilm is attached to a surface, it is quite difficult to remove. Mineral salt crystals, clay and silt particles etc. are also sometimes present within the biofilm matrix (depending upon the surroundings). The majority of biofilms found in the environment are either phytoplanktonic or bacterial (Donlan, Sept 2002).
Biofilms can form on many surfaces, including natural aquatic systems, human teeth, medical devices like artificial heart valves and catheters,
There are several types of treatment methods present but biological treatment methods have gained much traction in the recent years due to their low operation costs, comparatively benign effects on the environment and their ease of handling and maintenance. Biological wastewater treatment methods can be subcategorized into dispersed growth systems and attached growth systems. Biofilms fall under the latter category (Sehar & Naz, 2016)
In attached growth systems, microbes get attached to a surface to form a biofilm. The formation of the biofilm depends on factors like the type of surface used, presence of polymeric molecules on the surface and cell-cell interactions. Wastewater is passed through the surfaces on which the biofilms are attached. The microorganisms in the biofilm utilize the organic matter present in the wastewater and subsequently, the biofilms grow. There are four predominant reactor types that utilize this technology. Trickling Filters (TFs), Rotating Biological Contactor (RBC) Systems, Constructed Wetlands and Membrane Bioreactors (MBRs) (Sehar & Naz, 2016). Trickling Filters and Membrane Bioreactors are focused on in this
Biochemical tests are used to identify microbes in the laboratory to aid in pinpointing the different groups of bacteria. The bacteria vary in the cellular morphology and staining properties as well as structural and metabolic properties. Using biochemical testing, it permits a keener study at related organisms. In addition, the use of numerous color changes that occur with the test, allow to for a rapid identification of comparisons and variances of the bacteria that are tested.
There are numerous types of bacteria that can be found in every environment. Each bacterium has different morphology which includes shape, texture and pigment production. These bacteria also have different food requirements which are important in being able to identify a microorganism. Microorganisms are a diverse group containing all bacteria a single cell prokaryotic organism that is found in every type of environment, archea single cell microorganism that lacks nuclei and almost all microorganisms are protozoa a unicellular eukaryotic organism. By identifying the causative agent of a bacterium within an individual, an antibiotic can be developed to prevent health issues. Microorganisms are also used to make certain food products for human consumption. An example of this would be the production of yogurt. It has probiotics that help with digestive abnormalities amongst other things. Probiotics are microorganisms that are consumed to provide health benefits in the body. Probiotics work by replacing the disturbed microbe with ones that are useful to digest. With the methods that wer...
The word “remediate” means to solve a problem, so the word “bioremediation” refers to the use of biological organisms to solve an environmental problem. Bacteria, fungi, protists and other microorganisms in a non-polluted environment are constantly breaking down organic matter, and when the soil is polluted, some of the organisms may die, but others will still be able to break down the pollutants. Bioremediation provides organisms that can consume the pollutants with fertilizer, oxygen, and other conditions to encourage the rapid growth of these organisms. They then would be able to break down the organic pollutants at a correspondingly faster rate. There are two general ways in which bioremediation functions. One way is where specific survival conditions of a microorganism living in the soil are enhanced to increase the rate of a pollutant’s degradation. The second way is when specialized microbes are added to degrade the contaminant. This way is less common. For many types of polluted soil, bioremediation provides an excellent method of clean-up, but in some cases the pollutant is toxic even for the microbes. These pollutants include metals such as cadmium or lead, and salts such as sodium chloride. Although it may not work in all cases, bioremediation is considerably easier than other methods because it enhances the functions that the microbes already carry out in the soil. Along with being easier, it can be much less expensive because the soil does not have to be pumped out of the ground for treatment (Environmental Inquiry-Bioremediation). Serratia Marcescens is a bacterium that is commonly used for bioremediation.
Stephenson, R., & Blackburn, J. J. (1998). The Industrial Wastewater Systems Handbook. New York: Lewis Publishers.
Fluoride has been established for many years as an effective material in the fight against caries. Silver has been used in health care as an anti-bacterial agent in many medical applications such as silver sutures and silver catheters. Silver diamine fluoride was created to conjoin the effectiveness of both. According to a critical review by Rosenblatt et al (2009), “…silver interacts with sulfhydryl groups of proteins and with DNA, altering hydrogen bonding and inhibiting respiratory processes, DNA unwinding, cell-wall synthesis, and cell division (Pg #?).” It is through those means that pathogens are destroyed and biofilm formations are hindered. The study by Lou et al (201...
...nvironmental Microbiology. New York: A John Wiley & Sons, Inc; 1992. pp. 125?156. Accessed December 2, 2013.
We live in an environment full of microorganisms. These organisms may be pathogenic causing serious infections to humans and other living organisms, some just commensals while others are helpful in the food industry. The harmful effect of these organisms is a function of the condition that surrounds them at a particular time. For example, a favorable temperature, and the acidity or alkalinity of the medium in which they find themselves are some of the key factors that helps them multiply well enough to cause infection.
Biofilms are formed by a six step process. First is a reversible process, when an organic monolayer(made of polysaccharides or glycoproteins) absorbs to the surface, altering the chemical and physical properties of the surface. This makes the surface more conditioned and increase the chance that planktonic bacteria will attach. Secondly, also a reversible step, is when the free-floating or planktonic bacteria encounter the conditioned surface, and some attachment of the bacteria may occur. The third step is when the bacteria is left attached too long, then an irreversible attachment occurs. F...
Molinari, J., & Hart, J. (2010). How to Choose and Use Environmental Surface Disinfectants. Cottone's Practical Infection Control in Dentistry (Third Edition ed., pp. 185- 193). Philadelphia: Wolters Kumar Lippincott Williams & Wilkins.
Multiplication of attached organisms leads to confluent growth and biofilm formation. Adherent bacteria synthesise extracellular polymers.
The bacteria and wastewater is mixed in an aeration tank and therefore the contaminants are removed by action of sorption and series of breakdown by the bacteria.
Isolation of bacteria includes several techniques by which different bacterial colonies from a mixed culture can be separated. This isolation is important as it helps in studying the particular organism with its distinguished traits. Bacteria are in habit of living in an association with other organism/bacteria as this association will help in the better survival of an organism. These microbial populations will cooperate together and achieve better nutrients for each other because the waste of one may serve as a nutrient for the other. Similarly the waste from the metabolism of the one may provide the favourable condition to the other for growth.
The word biodegradable is a term used to describe materials or items that are friendly to the environment and are considered organic materials. These organic materials decompose back into the soil as nutrients and help minimize waste and pollution. “Organic materials “biodegrade” when they are broken down by other living organisms (such as fungi, bacteria, or other microbes) into their constituent parts, and in turn recycled by nature as the building blocks for new life. The process can occur aerobically (with the aid of oxygen) or anaerobically (without oxygen). Substances break down much faster under aerobic conditions, as oxygen helps break the molecules apart, a process called oxidation.” (Talk). Biodegradation has many factors that determine the rate at which organic compounds breakdown.
Although there are a lot of conventional water treatment methods available, but due to their disadvantages, there is a need for development of new and effective methods for disinfection of total waterborne pathogens in water treatment. In the near future, advanced oxidation process (AOPs) may become the most widely used water treatment technologies [30, 31, 32]. The processes can be categorized into three types:
Improvement and upgrading of wastewater treatment processes and also the need to reduce the environmental factors make the use of tertiary wastewater treatment important.