The Effect of Sucrose Concentrations (0.00 to 0.73 mol/L) on the Germination Rates of Raphanus sativus seeds.
Introduction:
Research Question: What impact does different sucrose concentrations (0.00 to 0.73 mol/L) have on germination and subsequent growth of Raphanus sativus seeds?
Background information: Germination experiments are typically completed in many high school science classes; such as in the prerequisite to IB biology, where students analyze the effect of salinity or radiation on germination rates. Many scholarly publications have been published testing the effect of salts on plant germination, yet there are very few that have been produced on the effect of sucrose. In the few experiments conducted testing the impact of sucrose,
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If the seed is germinated, measure the radicle and plumule using a ruler measuring in centimeters.
Safety, Environmental, and Ethical Concerns:
When preparing solutions, wear safety goggles to prevent sucrose concentrations from contaminating eyes. Also use precaution when handling the glassware, as broken glass can cause serious injury. After the experiment, sucrose concentrations can be disposed of into normal water treatment systems because the sucrose is a natural, non-toxic substance. Make sure radishes are properly disposed off once the experiment is completed. This experiment has no ethical concerns because there were no risk of disturbing a natural ecosystem because the experiment was conducted in a laboratory nor any animals used in this process.
The images below represent different steps of the experiment, beginning on the left with image 1, center is image 2, and the right is image 3. Image 1 Image
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Surprising, a significant increase in the molarity was required to see a difference in the measured dependent variable. Because the sucrose concentration changed the molarity of the solution, the data showed that the germination rate decreased when the molarity increased. The increase in molarity also had correlated shorter root and shoot lengths in the seeds that germinated. An ANOVA test was conducted to determine whether there is a significant difference in the data, similar to a combination of T-tests. In the ANOVA tests, the F-value for both the mean shoots and roots was larger than the critical F-value. This response suggests that there was a statistical difference between the control groups and the experimental group, which means the null hypothesis was rejected and the alternative supported. The line of regression allows for a visual representation to affirm the correlated decrease as the molarity increases. After conducting the ANOVA test and running regression lines through the data collected, there was a statistical difference between the control group and the experimental group. However, the data suggests that there is a decrease in the percent germinated, as well as a decrease in the mean root and mean shoot length, as an increase in sucrose concentrations
Thorough analysis of the graph displayed enough evidence suggesting that an increase in substrate concentration will increase the height of bubbles until it reaches the optimum amount of substrate concentration, resulting in a plateau in the graphs (figure 2). Hence; supported the hypothesis.
Abstract: Gibberellic acid is a plant hormone that is used to stimulate growth and fasten the germination of plants. When Gibberellic acid used on plants, it produces bigger and fuller leaves following by elongating the stems. This experiment was designed to determine the effect that Gibberellic acid will have on the growth of a seed germination. As performed in class, three types of radish seed were treated with Gibberellic acid to see the effects the acid will have on those three seeds. During the treatment plan, the three seeds received a different amount of acid and water five times a week. For instance, Seed A got approximately 2.5ML of Gibberellin acid, seed B got 5.0ML, and seed C got 10ML, following by seed A getting 17.5ML of water, seed B getting 15.0ML, and seed C obtaining 10ML of water. However, based on this treatment plan, seed A and B showed no growth. While, the seed that consumed more acid, which was Seed C showed rapid growth. In this case, the only possible explanation for this surprising result could be that the Seeds
The purpose of this procedure was to use the carbohydrate sucrose in a fermentation reaction in order to produce ethanol.
Our subject plant for this experiment is Helianthus annuus, a common sunflower plant cultivated all over the world for its seeds and seed oil. Helianthus is an annual plant that grows from 1 to 3 meters, depending on conditions. It is for this reason I chose Helianthus as our subject plant. Shorter plants have a less measurable variable. Helianthus plants, with their long straight stems, would have a much easier variable to measure. A total of 24 Helianthus plants, six to each pot, will be used in the treatment. The large number of subject plants is because of genetic variation that might sway my results if I were to use only a single treatment pot.
To investigate the osmotic effect of changing the concentration of sucrose solution; distilled water, 20% sucrose solution, 40% sucrose solution, 60% sucrose solution on the change in mass of potato cylinder after 30 minutes of being in solution.
Conclusions: There is a pattern on the graph, and data table, which shows that as the concentration of the sucrose solution increases, the potato's percentage change in mass decreases.
Two members of the group were instructed to visit the laboratory each day of the experiment to water and measure the plants (Handout 1). The measurements that were preformed were to be precise and accurate by the group by organizing a standardized way to measure the plants. The plants were measured from the level of the soil, which was flat throughout all the cups, to the tip of the apical meristems. The leaves were not considered. The watering of the plants took place nearly everyday, except for the times the lab was closed. Respective of cup label, the appropriate drop of solution was added to the plant, at the very tip of the apical meristems.
The primary nutrients that plants require are carbon, hydrogen, and oxygen from air and water. Sugar has a different effect on animals, humans, and plants. In this experiment, we are testing the effects of sugar on the growth of wheatgrass. For this experiment, we used regular sugar that we put in our food and drinks. To test our hypothesis we are using two groups control and experiment group. The control plant received only water but the experimental plant received sugar but, both plants were placed in the same temperature and same amount of water. Our hypothesis was correct, experiment plant “sugar water” yield more plant growth than control plant “water”. This experiment shows the sugar water plant grow faster than water because of the average of both plants. The sugar water experiment plant had longer in length compared to water control plant.
The Effect of Concentration of Sucrose Solution on Osmosis in Potato Tissue. Aim :- To find the effect of varying external concentrations of sucrose. solution of osmosis in potato tissue. Variables :- J. The concentration of the sucrose solution around the potato tissue. Depending on the concentration of the solution, osmosis will occur.
As evidenced by the data collected, different concentrations of sucrose do indeed affect the rate of CO2 production in yeast. When there is more sugar in a solution, more bubbles of CO2 are produced. This happens because of a anaerobic process called alcoholic fermentation, which is carried out by saccharomyces in order to convert sugars into ethanol and carbon dioxide [1]. In this particular experiment, the yeast in the conical flask was forced to perform fermentation because its access to oxygen was being restricted by a rubber bung. During the first step of anaerobic respiration, the yeast breaks down sucrose (a disaccharide) into glucose (monosaccharides) through the use of an enzyme named invertase [2].
Water Relations in Two Plant Tissues Results: Table 5 Table to show the sucrose concentration and water potential of each tissue. The sucrose solution was extrapolated from graph 1, which shows the percentage change of mass of the tissues when immersed in the different sucrose solutions. A line of best fit was drawn, where the line of best fit intercepts the x-axis (concentration of sucrose solution) is the sucrose concentration of the tissue because at this point there is no mass loss of gain (read off of y-axis).
Investigation of the Concentration and the Effect of Sucrose on Osmosis in Apple and Potato Tissues
Step 3: Measure and weigh the cylinders. Step 4: Pour out 50 ml from five different sucrose solutions into five beakers the concentrations of: 0% (distilled water), 0.25%, 0.5%, 0.75%, 1.0% Step 5: Put cylinders into sucrose solutions. Step 6: Leave solutions for 25 minutes. Step 7: Take cylinders out, re-measure and re-weigh each potato cylinder. Step 8: Redo steps 1-7 two more times.
An Experiment to Investigate Osmosis in Plant Tissue. Aim: To conduct an investigation to compare the osmotic behavior of the osmotic animal. two types of plant tissue in varying concentrations of sucrose. solution. Then we can find the solution.
To make it a fair test I will test each concentration three times and use the average. My tests will be accurate as I will be using a very accurate scale and precise syringes. To make my experiment accurate I will be using distilled water to make my sucrose solutions so there are no impurities that may affect my experiment and I will also measure my results to two decimal places. To make my experiment safe I will use goggles.