Figure 1 is an illustration of lab 1, the serial dilution performed for bacteria. This table is describing the number of colony forming units and the observations of the plates per dilution after a week. We didn’t stain the bacteria because time was limited and we would have only determined if they were gram positive or negative.
Lab 2. After 72-hours of incubation at 37°C the fungi plates were refrigerated for a week. They were then observed, and a plate count was performed. A geometric mean was used to summarize bacteria data because the data can have so much variability. Bacteria can grow at an exponential rate under the right conditions. The geometric mean value will not be influenced by large fluctuations from between one data point and the next (Table 2). After establishing the CFU’s, the fungi was then extracted from the petri dish, stained, and then observed through microscopy. Identified were mycelium, septate hyphae, non-septate hyphae, sporangiophore, bacteria, sporangium, and spores (Figures 1-16).
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This table is the data of the serial dilution performed per mL, it describes the CFU’s, the geometric mean, and observations.
The results for both labs were to determine the actual amount plated. This is the amount of dilution used to make the particular pour plate or spread plate. In order to do this we needed to use the following equation (Table 3):
Table 3 is the data from both labs determining the plate count per mL for both bacteria and for fungi. While doing lab 1 for bacteria we only tested each of the five dilutions on one plate; where as, for the second lab for fungi we tested four dilutions on two plates
5. Two or more samples may be applied to each plate if they are kept
We finally took 1ml of the 0.01% solution from test tube using the glucose pipette and adding it to test tube 4, we then used the H2O pipette and added 9ml of H2O to test tube 4 creating 10ml of 0.001% solution.
The first day an unknown sample was assigned to each group of students. The first test applied was a gram stain to test for gram positive or gram-negative bacteria. The morphology of the two types of bacteria was viewed under the microscope and recorded. Then the sample was put on agar plates using the quadrant streak method for isolation. There were three agar plates; one was incubated at room temperature, the second at 30 degrees Celsius, and the third at 37 degrees Celsius. By placing each plate at a different temperature optimal growth temperature can be predicted for both species of bacteria.
The purpose of this study is to identify an unknown bacterium from a mixed culture, by conducting different biochemical tests. Bacteria are an integral part of our ecosystem. They can be found anywhere and identifying them becomes crucial to understanding their characteristics and their effects on other living things, especially humans. Biochemical testing helps us identify the microorganism present with great accuracy. The tests used in this experiment are rudimentary but are fundamental starting points for tests used in medical labs and helps students attain a better understanding of how tests are conducted in a real lab setting. The first step in this process is to use gram-staining technique to narrow down the unknown bacteria into one of the two big domains; gram-negative and gram-positive. Once the gram type is identified, biochemical tests are conducted to narrow down the specific bacterial species. These biochemical tests are process of elimination that relies on the bacteria’s ability to breakdown certain kinds of food sources, their respiratory abilities and other biochemical conditions found in nature.
Having more than one plate will allow for more accuracy in the results or allow for a person to determine were they went wrong during the experiment.
The purpose of this laboratory is to learn about cultural, morphological, and biochemical characteristics that are used in identifying bacterial isolates. Besides identifying the unknown culture, students also gain an understanding of the process of identification and the techniques and theory behind the process. Experiments such as gram stain, negative stain, endospore and other important tests in identifying unknown bacteria are performed. Various chemical tests were done and the results were carefully determined to identify the unknown bacteria. First session of lab started of by the selection of an unknown bacterium then inoculations of 2 tryptic soy gar (TSA) slants, 1 nutrient broth (TSB), 1 nutrient gelatin deep, 1 motility
I began my test to classify my unknown bacteria by performing the Gram staining because according to the first period procedure of the laboratory manual and the Appending H, it was the first test that should be done to plan and proceed to the next tests. Washed bottle of distilled water, three slides, and Gram-staining reagents
The fungus sclerotinia sclerotiorum over winters as sclerotia either in the soil or in stubble at the soil surface (Morton and Hall, 1989). If the weather (moisture and temperature) is favorable, small mushroom-like structures called apothecia will be produced on the sclerotia. Each sclerotia can several apothecia. Apothecia can produce millions of spores called ascospores. Ascospores will be released in air when the apothecia is mature. Some ascospores land on canola plants and infect dead canola tissues like fal...
The purpose of this project was to identify unknown bacteria species from a mixed culture. The two unknown species were initially plated onto Tryptic Soy Agar (TSA), Eosin Methylene Blue (EMB), Mannitol Salt Agar (MSA), and blood agar plates to distinguish between the two different bacteria using colony size, color, shape, and growth characteristics. By identifying and inoculating the differing types of colonies, the two unknown bacteria were purified and able to be tested
If the amount of either hydrogen peroxide or yeast is different in any of the sections in the experiment then the results
The purpose of the study was to identify what are unknown bacteria by applying all the methods that we have learn in microbiology for the identification of are unknown. We apply the different test and be able to recognize the different characteristic of are unknown. Each test has its own purpose to help identify the bacteria by the reaction.
I identified the genus and species of an unknown bacterial culture, #16, and I applied the following knowledge of morphologic, cultural and metabolic characteristics of the unknown microorganism according to the laboratory manual as well as my class notes and power point print outs. I was given an incubated agar slant labeled #16 and a rack of different tests to either examine or perform myself; the tests are as follows: Gram Stain; Nutrient Gelatin Test; Carbohydrate Fermentation; Dextrose, Lactose and Sucrose; IMVIC tests; Citrate, Indole, Mythel-Red and Vogues Proskauer test; as well as a Urease and TSI Test. Materials and Methods/Results Upon receiving the Microorganism (M.O.) #16, I prepared a slide by cleaning and drying it. Then, using a bottle of water I placed a sterile drop of water on the slide and used an inoculating loop, flame sterilized, I took a small sample of the unknown growth in my agar slant and smeared it onto the slide in a dime sized circle and then heat fixed it for ten minutes.
3.) Divide your 30g of white substance into the 4 test tubes evenly. You should put 7.5g into each test tube along with the water.
According to the graph on amylase activity at various enzyme concentration (graph 1), the increase of enzyme dilution results in a slower decrease of amylose percentage. Looking at the graph, the amylose percentage decreases at a fast rate with the undiluted enzyme. However, the enzyme dilution with a concentration of 1:3 decreased at a slow rate over time. Additionally, the higher the enzyme dilution, the higher the amylose percentage. For example, in the graph it can be seen that the enzyme dilution with a 1:9 concentration increased over time. However, there is a drastic increase after four minutes, but this is most likely a result of the error that was encountered during the experiment. The undiluted enzyme and the enzyme dilution had a low amylose percentage because there was high enzyme activity. Also, there was an increase in amylose percentage with the enzyme dilution with a 1: 9 concentrations because there was low enzyme activity.
In this method, living spores which are resistant to whichever sterilizing agent is being tested are prepared in either a self contained system, such as dry sp...