Macromolecules In Food Lab Report

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Macromolecules in Food Materials and Methods For the first biuret test, approximately 10 drops of water were added to the test tube. After the water was inside of the test tube, 10 drops of 5M NaOH and 5 drops of the Biuret reagent solution was added. The test tube was then gently rolled by a member of the group to mix the solution and everyone recorded the color of the solution. The same procedure was followed to test for starch, protein, sugar, lipid, and the unknown, but instead of the initial 10 drops of water, a sample of the variable being tested was placed in the test tube. Results Tube # Contents Color Relative strength 1 10 drops starch solution and 10 drops 4M HCl Black, little orange ++++ 2 10 drops starch solution and 10 drops …show more content…

The unknown substance is probably a carbohydrate because it tested positive for starch which is a polysaccharide. This reaction also had very similar results as the Lugol’s test for potatoes which is a polysaccharide. Although the colors from the test for potatoes were not the same colors as the test for the unknown; the Biuret test had a slight color change and the Lugol’s test had a dramatic color change for both the unknown and potatoes. I am sure that the unknown was a starch, but the Benedict’s test for sugar was positive for the potatoes while the Benedict’s test for the unknown didn’t have a color change. The unknown probably did not have a color change for the Benedict’s test simply because there were not enough sugar present in the unknown for it to test positive. The Sudan IV Test for Lipids did not test positive for the unknown nor the potatoes because there isn’t a trace of lipids in starch. Based on my results, the unknown has a little protein, a lot of starch and no traces of lipids or

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