Hydrogen Peroxide Investigation
When hydrogen peroxide is added to a piece of fresh potato, bubbles of
gas are seen to form. When a bigger piece of potato is used, a larger
number of bubbles is produced. A possible explanation for this
observation could be that oxygen is produced when hydrogen peroxide is
broken down by the enzyme catalase. A large piece of potato will
contain a higher amount of catalase than a smaller piece and therefore
produces a greater volume of oxygen.
The Task
Design an experiment to show that an increase in the concentration of
catalase affects the volume of oxygen released from hydrogen peroxide.
Hypothesis
I predict that as you add more potato , the concentration of the
catalase increases resulting in an increase of the volume of oxygen
released from hydrogen peroxide.
Apparatus
Potato
Tile- to cut the potato discs
Borer (size 5)- so that the potatoes have a consistent diameter
Scalpel- to cut the potato cylinders into discs
Ruler- to measure the size of potato discs
Hydrogen peroxide
Beaker- to pour the hydrogen peroxide into so that it can be used
safely
Measuring cylinder- to measure the hydrogen peroxide
5 Test tubes (must be the same size so that the experiment is reliable)-
where the reaction will occur
Test tube rack- to put the test tubes in
Marker pen- to mark the test tubes
Gas syringe (with a rubber bung attached to it)- to measure the amount
of oxygen produced
Thermometer- to measure the temperature of hydrogen peroxide
Universal indicator
Goggles and overall
Paper & pen- to record results
Stop watch- to control the length of time the potatoes are in the
hydrogen peroxide before taking a measurement
My independent variable is the amount of potato discs i.e. catalase
that I will use. This will be varied by adding a different number of
potato discs to each test tube.
Data from Table 1. confirms the theory that as the concentration of glucose increases so will the absorbance of the solution when examined with the glucose oxidase/horseradish peroxidase assay. Glucose within the context of this assay is determined by the amount of ferricyanide, determined by absornace, which is produced in a one to one ratio.1 Furthermore when examining the glucose standards, a linear calibration curve was able to be produced (shown as Figure 1). Noted the R2 value of the y = 1.808x - 0.0125 trend line is 0.9958, which is statistically considered linear. From this calibration curve the absorbance values of unknowns samples can be compared, and the correlated glucose concentration can then be approximated.
Table 6 shows the results of the biochemical tests. The isolate can obtain its energy by means of aerobic respiration but not fermentation. In the Oxidation-Fermentation test, a yellow color change was produced only under both aerobic conditions, indicating that the EI can oxidize glucose to produce acidic products. In addition to glucose, the EI can also utilize lactose and sucrose, and this deduction is based on the fact that the color of the test medium broth changed to yellow in all three Phenol Red Broth tests. These results are further supported by the results of the Triple Sugar Iron Agar test. Although the EI does perform fermentation of these three carbohydrates, it appears that this bacterium cannot perform mixed acid fermentation nor 2,3-butanediol fermentation due to the lack of color change in Methyl Red and Vogues-Proskauer
way. I will put each piece of potato into the 5 test tubes and add the
In this experiment the enzyme peroxidase and the substrate hydrogen peroxide were not mixed initially, instead they were both placed in separate tubes and were incubated at a specific temperature, to prevent hydrogen peroxide from undergoing any reaction with peroxidase until they both acquire the required temperature.
If we say that the right hand side in picture 1 is the potato, and the
Test tube A will have a piece of potato which is this size. Test tube
Warm Potato: that when the potato is warmed but not heated over 35 degrees (due to high temperature will...
Investigate the Effect of pH on Immobilised Yeast Cells on the Breakdown of Hydrogen Peroxide
When you place a potato chip in a salt or sugar solution, then if the
The Effect of a Catalase on the Breakdown of Hydrogen Peroxide Aim To follow the progress of a catalysed reaction by measuring the volume of gas produced as the reaction proceeds. Using the initial rates of a series of experiments I will be able to find the orders of the reaction with respect to enzyme and substrate. Also to find out if concentration has an effect on the reaction when an enzyme is used to accelerate the breakdown of hydrogen peroxide.
Materials used in the experiment included 5-7 g of the potato tissue, 50ml of 2.0M phosphate buffer coffee filter and guaiacol dye.
water in the potato, then the water will go out of the potato and into
Investigating the Effect of the Enzyme Catalyse On Hydrogen Peroxide Introduction The aim of this experiment is to determine the effects of varying enzyme (catalyse) on Hydrogen Peroxide. Hydrogen Peroxide + Catalyse à Water + Oxygen 2H2O2 à H2O + O2 + Heat Apparatus & Diagram [IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE] Bung Potato Hydrogen Peroxide Water Collected Oxygen Delivery Tube Measuring Cylinder [IMAGE] Using the Equipment Safely It is important that we use the apparatus carefully, as safety will be an issue throughout the whole experiment. We will wear goggles and an apron or lab coat to protect our eyes and clothes. As we are using enzymes and Hydrogen Peroxide we need to be extra careful, ensuring they don't come into contact with our eyes, skin or clothes. Catalyse is an enzyme found in all living cells.
Type of tissue- this will be controlled by using only one type of tissue, which is the potato
This graph shows the result that I expect to get, I expect to see a