MTT assay was used to analyze cell viability of MCF 10A, MCF 7 and MDB-MB 231 cell lines in control and 10mM inorganic phosphate treated groups. MTT assay is a colorimetric assay for evaluating cell metabolic activity. MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) is a yellow aqueous tetrazolium dye. In living cells with active mitochondria, the mitochondrial enzymes such as succinate dehydrogenase (NADPH dependent oxidoreductase enzyme) reduce this dye to formazan, a purple-colored
Abbott Architect ci4100 is an automated diagnostic analyzer that integrates i1000SR immunoassay and clinical c4000 chemistry testing on one platform. This improves the performance and efficiency in the lab. This Architect Analyzer has a maximum throughput of up to 800 chemistry and 100 immunoassay tests per hour. An on- board reagent capacity of 55 chemistry, and 25 immunoassay kits. Load capacity of up to 180 samples that can be continuously loaded and unloaded during the testing process with
previously extracted protein by measuring the absorbance of the unknown amount and determining its concentration by overlaying it against a standard curve of the absorbance of known concentrations of the protein. We used the dye agent Bradford Protein Assay to get an absorbance of 0.078, 0.143, 0.393, 0.473, and 0.527 at the protein’s respective concentrations of 0.28, 0.56, 0.84, 1.12, and 1.40 mg/mL. When a best-fit line was applied to the standard curve, and the absorbance of our unknown concentration
laser on one side only and tumor growth was monitored. Finally, the tumors were removed and evaluated for tumor apoptosis. The results of the in vitro models was that endocytosis did occur in the cells treated with Cdot-Ce6-HA. Using a live/dead assay, these cells showed red fluorescence, suggesting cell death. Cdot-Ce6-HA also showed more photodynamic effect on the cancers cells, when compared to Ce6, and Cdot-Ce6. The in vivo model showed that transdermal delivery resulted in delivery of Cdot-Ce6-HA
Research Experience I have proactively engaged in research activity throughout my undergraduate program. I had my first research experience in Dr. B Anand’s lab, Indian Institute of Technology (IIT), Guwahati, during my second year. The aim of the project was to study the non-fluorescent beta-barrel structured proteins and engineer its amino acid sequence to make them into fluorescent ones. Our approach was mainly based upon the studies of Green Fluorescent Protein (GFP) from a jellyfish Aequorea
Statement of Purpose Having pursued relevant projects, courses and considering my inherent ability, I find myself interested in life sciences and more specifically in Microbiology. I believe that pursuing Masters in Microbial Biotechnology at North Carolina State University will help me widen my knowledge, hone my technical and managerial skills and provide me an opportunity to be a part of the cutting edge research in the field and contribute to its growth in Industry. Consistently a top student
Hypertension is one of the major risk factors for the development of cardiovascular diseases including stroke and may also have a role in the development of vascular cognitive impairment and vascular dementia [1, 2]. Angiotensin I-converting enzyme (EC 3.4.15.1; ACE) plays an important role in the rennin-angiotensin system and it is a carboxyl-terminal dipeptidyl exopeptidase that catalyzes the conversion of angiotensin I to angiotensin II [3-6]. ACE converts an inactive form of decapeptide, angiotensin