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in B5 medium supplemented with 1 μM 1-naphthaleneacetic acid (NAA) and 40 g L-1 sucrose. The cells were cultured for one day at 22°C with continuous illumination and shaking at 120g. Next, 10 μL of overnight cultured Agrobacterium transformed with respective vectors were added into the cell suspension and cultured for an additional two days. After co-cultivation, the cell suspension was washed thrice with 10 mL of JPL3 medium supplemented with Carbencilin (250 μg mL-1) by centrifuging at 100g for two
poisonous and is lethal if ingested; all parts of the plant are toxic including; roots, stems, flowers, leaves, and so on. While, every part of this plant is toxic it i... ... middle of paper ... ...SAVING FOXGLOVE. University of Southern California , 1 Jan. 2011. Web. 23 Apr. 2014. . Patil, JG, ML Ahire, KM Nitnaware, S Panda, VP Bhatt, PB Kishor, and TD Nikam. "In Vitro Propagation and Production of Cardiotonic Glycosides in Shoot Cultures of Digitalis Purpurea L. by Elicitation and Precursor Feeding