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Effect of concentration on rate of diffusion experiment
Rate of diffusion experiment
Colorimetric determination
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Introduction Fick’s Law is used to measure the rate of diffusion. Diffusion is the movement of molecules from a region of high concentration to low concentration (Smith, 2012). Diffusion is highly important to almost every living organism. With the aid of diffusion various substances are able to passively move through an organism (Anderson et al., 2012). This process of transporting materials throughout the body can occur without the organism having to expend much energy (Anderson et al., 2012). An example of diffusion would be when a human consumes a glass of water; it will be diffused into the blood stream quite rapidly without our bodies putting in any extra effort. Diffusion plays a big role for mammals in their lungs and the intake …show more content…
First a Fisher Brand Dialysis cellulose acetate membrane tubing (#21-152-14), 12,000-14,000 MWCO (molecular weight cut off), was pre-soaked in distilled water for 20 minutes prior to use, as the barrier between concentration one and concentration two. A grey plastic collar was inserted into the tubing (with the aid of distilled water). It was very important to insert the collar far enough to ensure the tubing completely covers it. A red rubber septum was then placed into the opening and folding the septum back over the collar created a tight …show more content…
The ½ concentration however begins to plateau at a faster rate then the full concentration, which takes a much longer period of time to reach its plateau (fig.1). The plateau for both of the different iodine concentration concludes that each system is reaching equilibrium and therefore returning to a homeostatic state. Fick’s Law can be used to better understand the patterns in figure 1. It states that the diffusion rate is equal to the difference in concentration. This means that because the difference in concentration between the two solutions (iodine and soluble starch) used in this experiment were greater when the iodine was at full concentration, the rate of diffusion would also be greater. This occurs as the molecules of the iodine want to go from a place of high concentration to that of low concentration therefore the change in absorbance will be larger as more molecules of are passing through the semi-permeable membrane. We can thus conclude that when the iodine solution is at a higher concentration it will have a higher average absorbance as a greater amount of iodine molecules are needed to pass through the membrane to become saturated and return the system to equilibrium (Fig.
Dialysis tubing is made from regenerated cellulose or cellophane, and is used in clinical circumstances to ensure that molecule have a filtered flow, and that larger solute molecules do not enter the dialysis tubing (Alberts, 2002). Like a cell membrane, dialysis tubing has a semi-permeable membrane, which allows small molecule to permeate through the membrane. Thus, the dialysis tubing mimics the diffusion and osmosis processes of the cell membrane (Alberts, 2002). Although the dialysis tubing has a semi-permeable membrane, which mimics a cell, its structure is different. The me...
In this investigation, the concentration of enzyme will be inversely proportional to the time taken for starch to be digested, until at a certain point where it will level out. It will level out because, all the substrates would have been used up, therefore there will be no more substrates for the enzymes to work on. In effect, the concentration of the substrate will act as a limiting factor. However, enzyme concentration will be directly proportional to the rate of reaction.
Investigating the Effect of Concentration on the Rate of Diffusion Aim: To find out if concentration affects the rate of diffusion. Prediction: I predict that the higher the concentration of acid the faster the reaction will be. Hypothesis: Diffusion is the spreading out of a gas or liquid from an area of low concentration to another area where it has a lower concentration until the overall concentrations are balanced. The Hydrochloric acid (HCl) diffuses into the gelatine cube of which contains Sodium Hydroxide (NaOH), which is an alkali. When the Hydrochloric acid combines with the Sodium Hydroxide they form salt and water, which is neutral therefore turning the pink cube to clear.
The side of the membrane that has the higher concentration is said to have the concentration gradient. It drives diffusion because substances always move down their concentration gradient. The pressure gradient also plays a role in diffusion. Where this is a pressure gradient there is motion of molecules. The pressure gradient is a difference in pressure between two different points.
* In the first dialysis tube (bag A), we would insert ten mL of one
The Importance of Diffusion to Living Organisms Diffusion is basically the movement of chemical species (ions or molecules) under the influence of concentration difference. The species will move from the high concentration area to the low concentration area till the concentration is consistent in the whole system. Diffusion mostly occurs in gases and liquids as these can move freely. The main features of an efficient diffusion system would be that it has a large surface area, thin membrane and a continuous supply of substances. A large surface area is needed so that high amount of substances can be exchanged at a time while the thin membrane means that the diffusion pathway would be short so that it is more efficient.
At point A the graph shows that no change in mass, of the potato, would have have occurred had we used a 0.2 (m) sucrose solution. This suggests that the concentration of water inside the potato would have been equal to the solution outside the potato. At point B (plain water), there is no indication that the cell is increasing in mass. This is because the cell is fully turgid and no more water can enter.
Hemodialysis uses a dialyzer, or special filter, to clean your blood. The dialyzer connects to a machine. During treatment, your blood travels through tubes into the dialyzer. The dialyzer filters out wastes and extra fluids. Then the newly cleaned blood flows through another set of tubes and back into your body.
This is because the water molecules pass from a low concentration to a high concentration, (in the potato chip). Therefore, the chips in low concentrations will gain mass and have a greater length height than in higher concentrations such as 1molar of sugar solution. If there is no gain or loss in height or mass then these will be the isotonic points of the potato cells. The isotonic point is where the cells are not increasing or decreasing in size and is known to be in a state of equilibrium. Apparatus: 1.
Investigating The Effect Of Concentration Of Salt Solution On The Mass Of Potato Chips Aim: The aim of this investigation is to see whether the amount of salt solution affects the mass of the potato chip Hypothesis: In the solution below, water will diffuse from the dilute solution into the concentrated solution through the process of osmosis. Therefore the dilute solution level will fall and the concentrated level will rise Line Callout 2: Disapproved (is unable to go through the semi permeable membrane O Line Callout 2: Approved (is able to go through the semi permeable membrane P Salt solution Water [IMAGE] When a substance such as salt dissolves in water, the substance's molecules stick with some of the water molecules, so the concentration of the water molecules decreases. When the water molecules are the same concentration on both sides of a semi permeable membrane and salt is dissolved into one of them, osmosis will occur as shown in the diagram below.
This dialyzer is a clear plastic tube which contains thousands of micro fibers that allow the blood to flow through while enabling diffusion to remove the unwanted components of the blood. The majority of dialysis patients use this as their primary method of treatment; however, some patients find the treatment to be harsh and uncomfortable. These patients often look for alternate treatments, such as peritoneal dialysis. The human body has the ability to be modified to allow for dialysis to occur within the peritoneal cavity instead of the extracorporeal method used in hemodialysis. This cavity is located in the abdomen and is accessed via a tube called a peritoneal dialysis (PD) catheter.
Now, assemble and arrange all of the needed supplies so that they are easily accessible. Connect the IV tubing to the solution bag and allow the fluid in the bag to run through the entire length of the tubing, also known as priming the tubing. When this is done, clamp the tubing closed. You will then need to tear several pieces of tape, six to eight inche...
However, in order to measure the rates of reaction, sodium thiosulphate and starch are added. Sodium thiosulphate is added to react with a certain amount of iodine as it is made. Without the thiosulphate, the solution would turn blue/black immediately, due to the iodine and starch. The thiosulphate ions allow the rate of reaction to be determined by delaying the reaction so that it is practical to measure the time it takes for the iodine to react with the thiosulphate. After the all the thiosulphate has reacted with the iodine, the free iodine displays a dark blue/black colour with the starch. If t is the time for the blue/black colour to appear, then 1/t is a measure of the initial rate.
The Effect of Solute Concentration on the Rate of Osmosis Aim: To test and observe how the concentration gradient between a potato and water & sugar solution will affect the rate of osmosis. Introduction: Osmosis is defined as, diffusion, or net movement, of free water molecules from high to low concentration through a semi-permeable membrane. When a substance, such as sugar (which we will be using in the experiment we are about to analyse), dissolves in water, it attracts free water molecules to itself, and in doing so, stops them from moving freely. The effect of this, is that the concentration of (free) water molecules in that environment goes down. There are less free water molecules, and therefore less water molecules to pass across a semi-permeable membrane, through which sugar molecules and other molecules attached to them are too big to diffuse across with ease.
== = This experiment is based on the concept of Osmosis. Osmosis is the diffusion of water molecules from a region of high water concentration to a low water concentration through a semi permeable membrane (in this case, the cell potato cell membrane). The cell walls of the potato cells are semi permeable meaning that water molecules (which are small) can fit through but other bigger molecules such as glucose cannot pass through. The water molecules can flow both ways through the membrane, letting molecules both in and out.