The Pros And Cons Of A Recombinant Plasmid

A recombinant plasmid are created by first using an enzyme that can identify and isolate specifically which gene that need to be cut. They are call restriction enzymes or restriction endonucleases, and more than 100 of these enzymes have been isolated. After the human gene (gene of interest) that codes for the desire trait is located on the chromosome restriction enzyme does it job, by cutting out the gene from the DNA. Now, the two ends of the human gene will be those that will link up with the open ends of the plasmid. An enzyme, DNA ligase, is used to couple each end of the gene to the open ends of the plasmid; this thus restores the circular DNA molecule with the human gene. Now the plasmid, with the human gene, is reinserted into the bacteria. They are then cultured and produced in large quantities of identical bacteria carrying the human gene. Now, these bacteria produce the human protein coded for by the spliced human gene. The protein is then isolated and purified and are ready to be injected into patients (crop, etc.) (Gish 1998).

Being able to do gene transfer gave us the ability to genetically engineer DNA and transfer it from one species to another, and the ability to share the same trait. There are many other ways to transfer gene; however, using bacterium who sole purpose is

Vaccine produce by genetic engineering allows the vaccine being produce to not have the complete form of the virus, taking way the virus ability to be harmful and becoming a live virus. Vaccines can be produced using recombinant DNA technology or using cell culture. Crop plants can bear cheaper bioreactors to produce antigen to be utilize as Edible vaccine. Edible vaccine are a cheaper alternative to recombinant vaccine. Transgenic plants are treated as edible vaccine. Transgenic bananas and tomatoes cure disease like cholera and Hepatitis-B