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Investigating acid rain and effect on seed growth
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The Effect of Acid Rain on Germination of Cress Seeds
Aim: to investigate that how the acid rain affects the germination of
cress seeds.
The factors that effect the germination of seeds are:
· Temperature
· Acid rain
· Oxygen
· Water
I will just see what effect does the acid rain have on germination of
seeds.
Apparatus:
· Petri dish (11)
· Cress seeds (20 11)
· Sulphuric acid (70cm³)
· Distilled water
· Measuring Syringes (11)
· Beakers
· Filter paper (11)
· Specimen tube (11)
· Pipette
Diagram:
Method:
1. I collected all the apparatus needed (see apparatus list).
2. I made a dilution series (see dilution series).
3. Placed the filter paper in the bottom of the petri dish.
4. Took 10cm³ of diluted acid from dilution of 0.010 and labelled it
0.010. I repeated this with every single dilution and petri dish. But
wash the syringe with water so there is no acid in the specimen tube.
5. Picked 20 cress seeds for each petri dish and placed in each petri
dish.
6. Took 3cm³ distilled water in the pipette and putted it onto the
seeds.
7. Check the seeds everyday for three days that if they have
germinated or not. Also add 3cm³ of distilled water to each petri
dish.
Prediction: I predict that the stronger the concentration of the acid
the fever seeds will germinate. The stronger the concentration of acid
the smaller the length of germination.
Fair test: to make the experiment a fair one I will control the amount
of water, temperature and concentration of the acid. I will use 20
cress seeds to get reliable set of results for each dilution.
Safety: to make the experiment I will follow these rules:
· Always wear safety glasses because of the acid.
· Wear the lab coat all the time in case the acid spills on you.
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To avoid contamination as best as possible, if washing hands according to SOP1 is not possible, wearing gloves and spraying with 70% ethanol is also acceptable for the procedure. Furthermore, not touching the ends of the needle and filter are absolutely essential in avoiding contamination thus applying them to the syringe whilst the ends are still in their sterile packaging will aid the prevention of contamination. When not in use placing the syringe on its plunger creates a smaller surface area for the contaminants to attach to as well. Loosening the lids of the broth tubes before placing in the Biohazard cabinet ensures easier removal when required thus less exposure time of the opened end to the environment allowing contaminants in.
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