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Anthrax disease RESEARCH PAPER
Introduction anthrax disease
Anthrax disease RESEARCH PAPER
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Bacillus anthracis (anthrax) is a bacterium that infects organisms through sporulation. Bacillus anthracis is can be aerobic (needs oxygen) or, facultative anaerobic (does not require oxygen). It is also a gram positive bacterium that can be found in the soil of humid and damp climates (Hicks, 2012). The bacterium is part of the species Bacillus Cereus (Koehler, 2009). It is known to infect life forms four different ways. Infection can be caused through digestion, eating food that is contaminated with the bacteria. Another way is through inhaling; which occurs through breathing in the spores. The third way of becoming infected is through the skin, known as cutaneous; this infection occurs through open wounds. The newest form discovered of infection is through injection; the use of needles (Hicks, 2012). Though it is not common to be found in wealthy countries; there have been numerous outbreaks in Europe and the United States. It is also being considered for bioterrorism (Sweeney, 2011). Symptoms of the early stages of anthrax can be misunderstood for less lethal conditions (Hicks, 2012). For patients with a possible anthrax infection can be determined by gram positive staining. It can also be diagnosed by confirmatory testing, such as PCR (Polymerase Chain Reaction) (Sweeney, 2011). PCR focuses on certain DNA strands for replication and identifying what the organism is (Genetic, 2014). Anthrax toxin antagonists can be considered for healing; but antibiotic therapy is the most common use of treatment (Sweeney, 2011).
Bacillus anthracis is a non-motile elongated rod-shaped bacterium that will generate dormant spores with or without oxygen. When the spores are exposed to high levels of carbon dioxide and warmth they adjust into a ro...
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... four deaths in England, and one death in Germany from injected anthrax. Tissue swelling and tissue infection of the injection site occurred from the first to tenth day after injection were reported in nine out of ten cases. Color change in the skin from anthrax is unnoticeable due to it being similar to injecting heroin. Other reported symptoms are excessive bleeding and edema; if not treated the infected person would begin to develop organ failure (Hicks, 2012).
In conclusion Bacillus anthracis is a bacterium that lives thrives in soil. It can infect its hosts in many ways. It can be lethal if not treated with the right antibiotics or surgery. Outbreaks are not common but when they did occur is was due to vegetative spores that were released from the bacterium. Anthrax infections can be complicated and symptoms can either be noticeable or not. It can be dangerous.
After the end of the experiment the unknown 10 sample was Staphylococcus epidermidis. Came to this conclusion by first beginning with a Gram Stain test. By doing this test it would be easier to determine which route to take on the man made flow chart. Gram positive and gram negative bacteria have a set of different tests to help determine the unknown bacterium. Based on the different tests that were conducted in lab during the semester it was determined that the blood agar, MSA, and catalase test are used for gram positive bacteria while Macconkey, EMB, TSI, and citrate tests are used for gram negative bacteria. The results of the gram stain test were cocci and purple. This indicated that the unknown bacteria were gram positive. The gram stain test eliminated Escherichia coli, Klebsiella pneumonia, Salmonella enterica, and Yersinia enterocolitica as choices because these bacteria are gram negative. Next a Blood Agar plate was used because in order to do a MSA or a Catalase test there needs to be a colony of the bacteria. The result of the Blood Agar plate was nonhemolytic. This indicated that there was no lysis of red blood cells. By looking at the plate there was no change in the medium. Next an MSA test was done and the results showed that there was growth but no color change. This illustrates that the unkown bacteria could tolerate high salt concentration but not ferment mannitol. The MSA plate eliminated Streptococcus pneumonia and Streptococcus pyogenes as choices since the bacteria can’t grow in high salt concentration. Staphylococcus aureus could be eliminated because not only did the unknown bacteria grow but also it didn’t change color to yellow. Lastly a Catalase test was done by taking a colony from the Blood Agar plate...
In this experiment the heat shock method will be used to deliver a vector (plasmid) of GFP to transform and grow E. coli bacteria. Four plates containing Luria Bertani (LB) broth and either –pGLO and +pGLO will have E. coli bacteria added to it. The plate containing –pGLO (no pGLO) and LB will show growth as ampicillin will be present killing bacteria but no glowing because no arabinose will be present for glowing to be activated, the same result will be seen in the plate containing +pGLO, LB and ampicillin. The plate with –pGLO, LB and ampicillin will show no growth and no glowing as no arabinose is present for glowing to be activated
Bacillus globigii. (n.d.) WordNet 3.0, Farlex clipart collection. (2003-2008). Retrieved March 20 2014 from http://www.thefreedictionary.com/Bacillus+globigii
The 2001 anthrax attacks was one of the worst bio-weapon attacks on the US in history. The attacks where done through the mail. The anthrax was placed in envelopes with a letter and mailed from various locations to different people and organizations. The anthrax filled letters ended up killing 5 people, causing 17 to become sick and exposing anthrax it is believed to as many as 30,000 people. During the mail process spores of anthrax from the letters escaped and got on mailroom equipment exposing postal employees. If a person was exposed to enough anthrax and developed symptoms they typically died in a few days. Postal workers during the attacks where told that anthrax will appear as a white powder t...
Objective data are observable and measurable. This information is usually obtained through the senses as sight, smell, hearing and touch, during the physical examination of the patient. During Mary was assessed, this is the objective data: symmetrical abdomen, bowel sounds in all quadrants, tender to palpation in the lower quadrants, guarding, skin is warm and moist and her lips and mucous membranes are dry.
Linkous, J. (2004). More details on new anthrax search. Retrieved Oct. 06, 2005, from CBS News Web site: http://www.cbsnews.com/stories/2004/10/05/national/main647441.shtml.
Necrotizing Fasciitis (flesh eating bacteria ) from an essay by Katrina Tram Duong, edited by S.N. Carson M.D.
Bacillus anthracis or anthrax is bacteria that infect thousands of people a year worldwide. Mostly found in the hinds and hair of grazing animals like cattle and sheep, the bacteria can stay dormant for years until contacted. Contact can occur through broken skin, eating infected meat, or inhaling infected dust from hides through the mouth or nostrils. Normal contact through the skin begins forming malignant skin ulcers that are dark black in appearance. If left untreated, the blisters lead to blood poisoning and eventually death. Eating contaminated meat not fully cooked can cause intestinal infection also leading to death. Surprisingly, many people survive contact of the skin and very few cases of intestinal infection exist. However, inhalation of infected dust or spores are much more deadly because the symptoms resemble a common cold...
Louis Pasteur, in 1881, discovered the anthrax vaccine. Anthrax is an infection caused by many bacteria cells called “Bacillus”. These bacteria make “spores” which have a protective shell. This disease is most common in farm animals. They live in soil, and affect domestic a...
...merithrax Investigation :. : Journal of Biosecurity, Biosafety and Biodefense Law., 31 July 2012. Web. 23 Apr. 2014. .
Anthrax is a very real and dangerous threat to all people who come in contact with it. The bacterial infection is caused by the bacteriaium Bacillus anthracis. Anthrax is divided into three categories all are contracted in different ways. The first type is called Cutaneous Anthrax; this is where anthrax spores make contact with the skin. If there is a cut or a lesion then a person can be infected. Cutaneous Anthrax is the most common way to get anthrax, but is also the least lethal. The second type of anthrax is called Pulmonary Anthrax this is where a person inhales the spores of anthrax directly into the lungs. If this type of anthrax is left untreated it could lead to death. The third type of anthrax is called Gastrointestinal Anthrax, this form results from eating meat from animals that have been infected with the anthrax bacteria. This causes the intestinal tract to become infected and this from is almost always fatal. This particular mode of anthrax has never been seen in the United States.
Bacterial cells, like plant cells, are surrounded by a cell wall. However, bacterial cell walls are made up of polysaccharide chains linked to amino acids, while plant cell walls are made up of cellulose, which contains no amino acids. Many bacteria secrete a slimy capsule around the outside of the cell wall. The capsule provides additional protection for the cell. Many of the bacteria that cause diseases in animals are surrounded by a capsule. The capsule prevents the white blood cells and antibodies from destroying the invading bacterium. Inside the capsule and the cell wall is the cell membrane. In aerobic bacteria, the reactions of cellular respiration take place on fingerlike infoldings of the cell membrane. Ribosomes are scattered throughout the cytoplasm, and the DNA is generally found in the center of the cell. Many bacilli and spirilla have flagella, which are used for locomotion in water. A few types of bacteria that lack flagella move by gliding on a surface. However, the mechanism of this gliding motion is unknown. Most bacteria are aerobic, they require free oxygen to carry on cellular respiration. Some bacteria, called facultatibe anaerobes can live in either the presence or absence of free oxygen. They obtain energy either by aerobic respiration when oxygen is present or by fermentation when oxygen is absent. Still other bacteria cannot live in the presence of oxygen. These are called obligate anaerobes. Such bacteria obtain energy only fermentation. Through fermentation, different groups of bacteria produce a wide variety of organic compounds. Besides ethyl alcohol and lactic acid, bacterial fermentation can produce acetic acid, acetone, butyl alcohol, glycol, butyric acid, propionic acid, and methane, the main component of natural gas. Most bacteria are heterotrophic bacteria are either saprophytes or parasites. Saprophytes feed on the remains of dead plants and animals, and ordinarily do not cause disease. They release digestive enzymes onto the organic matter. The enzymes breakdown the large food molecules into smaller molecules, which are absorbed by the bacterial cells. Parasites live on or in living organisms, and may cause disease. A few types of bacteria are Autotrophic, they can synthesize the organic nutrients they require from inorganic substances. Autotrophic bacteria are either photosynthetic or Chemosynthetic. The photosynthetic bacteria contain chlorophyll that are different from the plant chlorophyll. In bacterial photosynthesis, hydrogen is obtained by the splitting of compounds other than water.
In this method, living spores which are resistant to whichever sterilizing agent is being tested are prepared in either a self contained system, such as dry sp...
The duration of the experiment should be increased as the thermal death times of B. subtilis at 60, 70 and 80°C were unable to be determined within 110 minutes. The duration can be increased to 180 minutes so as to better investigate its thermal death times. If the presence of bacterial growth was still observed after 180 minutes of exposure, it can be assumed that B. subtilis is able to survive well in that temperature. An exposure time of one day can be carried out to confirm this assumption.
Thesis: Biological Warfare is morally and inhumanely wrong, It is the wrongful killing of men, women, and children. It should be stopped no matter what the circumstances are.