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Photosynthesis experimental design
Photosynthesis experiment analysis
An experiment to investigate photosynthesis
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The Effects of CO on the Rate of Photosynthesis in Spinach Leaves
Bailey Wells
Abstract
Introduction
The experiment was conducted using carbon dioxide to see how it affected the rate of photosynthesis in spinach leaves. Carbon dioxide should increase the rate of photosynthesis because there will be more carbon dioxide, a reactant in the photosynthesis formula.
light
Carbon Dioxide+Water → Glucose+Oxygen
Materials and Methods
Materials Needed
Soap Solution
Sodium bicarbonate (baking soda)
Spinach Leaves
Water, distilled
Balance
Cups
Hole-punch
Light source
Paper Towels
Permanent Marker
Ruler
Syringe
Syringe Tip Cap
Support Stand
Timer
Methods
Step One: Using a hole punch cut out ten discs
from the leaves for each cup Step Two: Prepare the two solutions; one with an excess of CO2. The carbon dioxide concentration determines the amount of leaves that with float over set period of time. Step Three: Add one drop of liquid soap to each cup to wet the hydrophobic surface of the disks so they’ll sink Step Four: Draw the gases out of the spongy mesophyll tissue and infiltrate the leaves with the sodium bicarbonate solution and then pour the solution in to the appropriate cup Step Five: Put the cups under the light source and record the number of leaves that have floated to the top of the cup at each minute until all of the leaves have floated to the top, or there is no activity in the cups anymore Results Minutes CO2 Control 1 0 1 2 0 2 3 0 6 4 0 8 5 0 8 6 2 8 7 2 8 8 3 8 9 6 8 9:46 10 8 10 10 8 11 10 8 11:20 10 10 As the carbon dioxide concentration increased throughout the cups, the ET50 or the amount of time it took 50% of the leaf disks to float increased. As shown in Figure Two, at Cup 2 (the first cup with conclusive data) the ET50 was 8 minutes and at Cup 5 with a 1.0% carbon dioxide concentration the ET50 was 13 minutes showing that the data values of ET50 increased as the carbon dioxide concentration was increased. Discussion and Conclusion Based on the data the hypothesis was rejected. This research shows that the experiment was incorrectly done, as the hypothesis should have been supported not rejected. Weaknesses in the experiment were attempting to make the leaves sink by using a syringe to draw all air out of the solution combined with the ten leaves. For each cup not all of the leaves would sink after using the syringe. The purpose of this experiment was to determine if the concentration of carbon dioxide is increased with the increase in the rate of photosynthesis. The experiment should have worked but didn't possibly due to difficulties with the syringe
Increasing the light intensity will make photosynthesis faster. Variables: In this experiment there are a few things we have to keep the same.
The “Fast Plant” experiment is an observation of a plants growth over the span of twenty-eight days. The objective is to observe how plants grow and use their resources throughout the span of their life. In our lab we observed the Brassica rapa, a herbaceous plant in the mustard family which has a short cycle which makes it a perfect plant to observe in this experiment. Like other plants the Brassica rapa must use the resources in the environment to create energy to complete itʻs life cycle and reproduce. By observing the plant it is easy to see in what organ or function the plant is using itʻs energy and resources and if overtime the resources switch to other part of the plants. By conducting this experiment we are able to observe where and how plants allocate their resources throughout their life by harvesting plants at different points in their life.
Carbon dioxide is necessary for plants to carry out the process of photosynthesis, which is an important process because it allows plants to produce glucose, as well as oxygen. Based on evidence from previous experiments, the growth of the plant will reflect the amount of CO2 gas that is present in the environment (O’Leary and Knecht 1986). This means that the plant growth relies on the carbon dioxide in the atmosphere in order to be successful. Therefore, in several cases, it has been noted that when the concentration of CO2 is doubled, the plant growth will similarly increase (Carter et al. 1997). So, it can be concluded that by raising the amount of carbon dioxide in an environment, the surrounding plants will experience an increase in growth.
In this experiment I will investigate the affect in which the light intensity will have on a plants photosynthesis process. This will be done by measuring the bubbles of oxygen and having a bulb for the light intensity variable.
Experiment #1: The purpose of this experiment is to investigate the effects of baking soda and light intensity on the rate of photosynthesis of green spinach leave through the observation of floating disk.
The Effect of Light Intensity on the Rate of Oxygen Production in a Plant While Photosynthesis is Taking Place
The question we examined was how are photosynthesis and cellular respiration related? My hypothesis stated that if bromothymol blue, carbon dioxide, water and an Elodea plant are placed into test tube that is exposed to sunlight, then the bromothymol blue would change color which means that the plant has produced oxygen. When Bromothymol Blue is the color blue that indicates that oxygen is present. When Bromothymol Blue is the color green that signifies carbon dioxide is present. On Day 1, the Bromothymol Blue in all the test tubes was a orangish-yellowish-brown color. However, when we examined the Bromothymol Blue on day 2, in the control group, the color stayed same, a orangish-yellowish-brown. In the light test was bluish green and in the
= > [CH2O} + O2 + H2O, This shows that when the light intensity is increased the rate of reaction will be more quicker he only anomalous result there was, is the one in the 100 watt result the reading after 5 minutes is anomalous because it does not follow the predicted pattern of increasing in the production of gas because it is lower I know from my own knowledge of photosynthesise that when the light intensity is increased the rate of reaction will be more quicker because many plants and trees photosynthesise quicker in stronger light and photosynthesise slower in dimly lit places. The chlorophyll absorbs light energy and enables it to be used by the plant for building up sugar. The overall effect is that energy is transferred from sunlight to sugar molecules.
To begin we made sure that the spectrophotometer was warmed up and was set to 605 nm while the chloroplast suspension was being made by the instructor. 605 nm was used because this is the wavelength that is the easiest to see transmittance of DPIP in the chlorophyll. The chloroplast suspension is created using spinach leaves that are deveined because the veins contain less chloroplasts. Once the leaves had been deveined they were placed under a light to begin the photosynthesis process. Then the instructor then placed the leaves in a chilled blender. He used a chilled blender because the motor of the blender when in use would generate heat and the heat would in turn cause the leaves to denature. Next, he added .5 M sucrose to the blender which
The Effect of Light Intensity on Photosynthesis Of Elodea Canadensis Introduction I wanted to find out how much the light intensity affected the Photosynthesis in Elodea Camadensa. I decided to do this by measuring the amount of oxygen created during photosynthesis. Photosynthesis is the procedure all plants go through to make food. This process uses Carbon dioxide, water and light energy. It produces Oxygen and Glucose.
The Lettuce Challenge Experiment Design faced several errors that could have altered the course of the experiment. The experiment had tolerable results, however, they may have been more accurate if the errors had been refined. The first error that was noticed was the fact that the lettuce was not watered during the weekend. In addition, the lettuce was not given extra water on friday. The lack of water could have affected the growth of the plant. The amount of water given to the experimental plant differed from the control as well. The control was given 10 extra ml of water on friday while the experimental plant was not. Because the amount of water given to the control was different to the plant that was experimented
* Count the number of bubbles seen in 1 minute which is a way of
In our Biology Lab we did a laboratory experiment on fermentation, alcohol fermentation to be exact. Alcohol fermentation is a type of fermentation that produces the alcohol ethanol and CO2. In the experiment we estimated the rate of alcohol fermentation by measuring the rate of CO2 production. Both glycolysis and fermentation consist of a series of chemical reactions, each of which is catalyzed by a specific enzyme. Two of the tables substituted some of the solution glucose for two different types of solutions. They are as followed, Table #5 substituted glucose for sucrose and Table #6 substituted the glucose for pH4. The equation for alcohol fermentation consists of 6 Carbons 12 Hydrogens 6 Oxygen to produce 2 pyruvates plus 2 ATP then finally the final reaction will be 2 CO2 plus Ethanol. In the class our controlled numbers were at Table #1; their table had 15 mL Glucose, 10 mL RO water, and 10 mL of yeast which then they placed in an incubator at 37 degrees Celsius. We each then measured our own table’s fermentation flasks every 15 mins for an hour to compare to Table #1’s controlled numbers. At
An Experiment to Investigate the Effect of Light Intensity on the Rate of Photosynthesis. Introduction Photosynthetics take place in the chloroplasts of green plant cells. It can produce simple sugars using carbon dioxide and water causing the release of sugar and oxygen. The chemical equation of photosynthesis is: [ IMAGE ] 6CO 2 + 6H20 C 6 H12 O 6 + 6O2 It has been proven many times that plants need light to be able to photosynthesize, so you can say that without light the plant would neither photosynthesize nor survive.
This is the same if there is the light intensity is too high as this can damage the chloroplasts in plants and this will minimize the rate of photosynthesis. As shown in the graph as the light intensity increases, the photosynthesis rate increases until a point is reached where the rate begins to level off into a plateau. At a low light intensity, photosynthesis occurs slowly because only a small quantity of ATP and NADPH is produced. As the light intensity shown in the graph is gradually increasing, more ATP and NADPH (NADH is used in cellular respiration and NADPH is used in photosynthesis) are produced, which means more oxygen and sugar is produced, therefore increasing the rate of photosynthesis. But as the light intensity increases even more and past a certain light intensity on the graph, this is due to the other factors such as carbon dioxide limiting the rate of