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Essay of light microscope
Essay of light microscope
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Question 4
Compound Light Microscope is a device that uses visible light and magnifying lenses to search tiny objects that are not visible to the naked eye, or finer detail than the naked eye allows. Samples you can use with this device is a leaf, bug, or feather, but it is very blurry and pale color. The microscope works by an eyepiece, ocular lens, the field of view, focus dials, supporting arm, rack stop, objective lenses, slides, cover slips, and many other things. It works by the light source is below the stage, and this light shines up through the thin specimen and then through the magnifying lenses. Prepared the specimen for viewing by placing it on a regular glass side. It can be stained with a dye that contract to nearly transparent
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cells. Scanning Electron Microscope is used to view the surface of a specimen in a great detail of information. Some samples this device can use are a leaf, blood, or algae, but it looks like a 3-D Black and white specimen. This one works by an electron gun, specimen stage, Robinson detector, and an infrared camera. It works by looking or studying the small details and complex structures inside cells until the development of electron microscopes. Question 5 The types of specimens I was able to view under each of the microscopes in the activity were leaves, bugs, feathers, blood, and algae.
The specimen's views were different every time because each of the microscopes had a different view of each object. Dissecting Microscope looks blurred looking and pale color. Compound Light Microscope looks blurry looking and grows differently when you change the zoom or size. Transmission Electron Microscope looks grayish (black and white) and shows a lot of the structure of the specimen. Scanning Electron Microscope looks like a 3-D black and white shaped specimen. A leaf under a dissecting microscope would be described as this if you zoom in and out, you would see blurry, clear, then blurry again and it was bright then pale colors. Blood under a compound light microscope would be described as this if you look at it, it would be a blur and sometimes clear shape depends on the zoom in or out. Algae under a transmission electron microscope would be described as this if you look at it, it would be grayish different look by the shape and detailed with the structure of the thing. A leaf under a scanning electron microscope would be described as this if you zoom in and out, you would see blurry 3-D black and white with lots of detail of the
sample.
Around 1902, when luminol was first synthesized, scientists noticed that it exhibited a blue glow in the presence of other compounds. Later on, it was found that the luminol reaction occurred in the presence of blood. Its use in crime scenes was first implemented by German forensic scientist Walter Specht in 1937. When the luminol solution is sprayed, the iron present in hemoglobin in blood catalyzes the reaction to produce a blue glow. This is used because in certain situations, there may be too little blood present at a crime scene to be able to be seen. It can also help when a suspect had attempted to “clean up” the blood after committing the crime. In these cases, luminol can be used to see any evidence of blood that cannot be seen with the naked eye. The glow from the reaction only lasts for about thirty seconds and requires dark conditions to be able to see, but investigators are able to document it in
One definition of a microscope is "an optical instrument that uses a lens or a combination of lenses to produce magnified images of objects." Holden Caufield can be symbolized by a microscope and its parts: the field of view, the focus, and the magnifier.
Luminol is a white, sometimes pale yellow, crystalline solid that can create a chemiluminescence when mixed with certain oxidizing agents. In order to create luminol, there needs to be a diamindation of 3-Nitrophthalic Acid by Hydrazine, which produces 3-Nitrophthalihydrazine and is then reduced with Sodium Dithionite. The product on its own is not soluble in water but is soluble in most organic solvents. Other than emitting a blue light, luminol is also used in forensic studies. A forensic investigator can use luminol to detect traces of blood by spraying it on an area since it traces an activation oxidant in order to make it emit light. In order to create effective results, investigators must spray even amounts of the solution. The intensity
Comparing the Light and Electron Microscope In this essay I am going to be comparing the light and electron microscope, I will look at the advantages and disadvantages of each microscope and then analyse my findings to see if one is better than the other. The light, or optical microscope as it is also known was invented in the 17th century, it has been refined in many ways over the years but it is essentially still the same. The light microscope works by; light rays from a light source beneath the stage are through to glass lenses in series. The two lenses are called the objective lens and the ocular (eyepiece) lens.
MRI is a procedure, in wide use since the 80s, to see the anatomy of the internal organs of the body. It is based on the phenomenon of nuclear magnetic resonance (NMR), first described in landmark papers over fifty years ago (Rabi et al. 1938; Rabi, Millman, and Kusch 1939; Purcell et al. 1945; Bloch, Hansen, and Packard 1946) (4 ). . The MRI is a valuable diagnostic and research tool with also practical applications for surgical planning and conquering diseases. This imaging procedure is painless and non-invasive although sometimes discomforting as the patient lies down in a body tube that surrounds them. For many years, closed MRI units have been the standard in helping physicians make a diagnosis. These closed MRI units featured a long tube that the patient would be placed inside during their procedure. This was often uncomfortable for many patients due to the "closed in" feeling and was especially stressful for patients who suffer from claustrophobia. The newest generation of MRI units is now open on all four sides which completely alleviates the "closed in" feeling, while still providing the physician with the most accurate information possible to aid in diagnosis (2).. A patient does not see or feel anything. A faint knocking sound may be heard as the machine processes information. Patients may choose to listen to music -- even having the option of bringing their own CDs to listen to. Most MRI procedures take less than an hour. MRI technology is based on three things: magnetism, radiofrequency and computers. The magnetic resonance machine, is a big and strong magnet. When the body is inside, every proton of the body is oriented in the same way (for instance, with the positive pole up). Water ...
Apfeldorf’s article “Uncovering a Tiny World” discusses Hooke’s book which is known for its microscopic illustrations of insects and microbes that Hooke had drawn as he viewed them under the microscope. His elaborate drawings of tiny objects and insects were the scientific evidence that supported his claims of the significant value of the microscope to science and the many ways it could be used. The book also contained a description of how to make a powerful microscope with a spherical lens, much like Leeuwenhoek’s glass pearls. Leeuwenhoek traveled to England that same year and is believed to have obtained a copy of Hooke’s book and
One of the most recently new advances in radiology is the use of magnetic resonance imaging (MRI). MRI has been around for the past century. It was at first called Nuclear Magnetic Resonance (NMR) and then it changed to MRI once there was an available image. Walter Gerlach and Otto Stern were the first scientists to start experimenting with the magnetic imaging. Their very first experiment was looking at the magnetic moments of silver by using some type of x-ray beam. The scientists then discovered this was by realizing that the magnetic force in the equipment and in the object itself. In 1975, the first image was finally created using and MRI machine. The scientists used a Fourier Transformation machine to reconstruct images into 2D. The first images ever use diagnostically was in 1980. This is when hospitals began to use them. At first the images took hours to develop and were only used on the patients that needed it most. Even though MRI has been around for a long time, it has advanced and has been one of the best imaging modalities recently (Geva, 2006).
the spores through the micro scope. It was to soon to see anything but little
Positron-emission tomography (PET) is a nuclear medicine imaging modality which detects gamma rays emitted by a positron-emitting radioactive tracer. The most common tracer used for neuroimaging is 2-deoxy-2 (18F) fluoro-d-glucose (FDG). It approximates for the metabolic processes in the brain providing a broad range of functional and metabolic information to help understand mechanisms of neurologic diseases and guide therapeutic approaches. Most settings have used 2-deoxy-2 (18F) fluoro-d-glucose (FDG) in the interictal state [35,47]. Ictal PET is not of much help as the half-life of radiotracer used extremely short. For patients with temporal lobe epilepsy interictal studies shows hypometabolism in epileptogenic areas. Interictal FDG-PET provides more information about epileptogenic foci compared to
The two types of microscopes that will be focused on in this webpage are the simple microscope and the compound microscope. The simple microscope, also known as the magnifying glass, is composed of a single converging lens. The compound microscope is composed of at least two lenses and is generally referred to as a microscope.
Anatomists, morphologists and biologists have tried to understand the way living creatures are living, and what they look like inside. Dr. Frourie in 1974 painfully cut organisms into thin slices, to study their internal arrangement to better understand them. Today, these people use CT scanners instead. It is a lot easier, less bloody, takes shorter time and the sample is not affected or destroyed during the procedure- the advantages are numerous. This technique enables us to visualize the inside of us humans, organisms or other objects in 3-D geometry.
Compound Microscopes have assisted scientists in the research of objects invisible to the naked eye for more than four hundred years and have greatly influenced our understanding of the world around us. As technology has progressed, Light Microscopy has significantly improved. These improvements include illumination methods, the Resolution lens quality and the use of oil immersion.
The scientists observed the small ripples on the cell membranes under a high-contrast differential interference contrast, which is a type of microscope that is good to use for observing cells.
the bulk to ordinary matter; the volume of an atom is nearly all occupied by the
Before the discovery of X-rays in 1895, it was impossible to look inside human body, without causing harmful side effects. The famous quote of Anna Bertha Ludwig - “I have seen my death” is a testimony to this. In ancient times, the only way to study internal human organs was the dissection of dead bodies. Additionally, this was also subject to availability or religious beliefs. Leonardo da Vinci made 240 detailed sketches between 1510 and 1511, which were way ahead of their time. Unfortunately, it could not be published, except for a small amount in 1632. Images aide in visualization of illnesses (e.g. a malignant tumor), which are impossible to observe from outside of the body. A surgeon must know the various attributes of the tumor like location and size, before she can operate on it. Similarly an oncologist needs this information to decide the course of treatment e.g. tumor size and metabolic activity may be needed to determine the number of chemotherapy sessions. With images, all this information can be obtained without cutting open the patient. And what’s remarkable is that u...