Post-Moortem Cases

Lovas JGL, in 1986 conducted a study, apoptosis in human epidermis by electron microscopy, on skin samples excised from the middle of the medial aspect of the upper arm of m106 consecutive autopsy cases. The time interval between death and biopsy was less than 24 hours in all but three cases. The tissue was fixed overnight in, the tissues were stained Thick sections for light microscopic (LM) survey were stained with toluidine-blue. Thin sections for EM were stained with uranyl acetate and lead citrate, and examined using a Zeiss EM 109 transmission electron microscope. He observed Apoptotic keratinocytes in 95 of the 106 cases. Electron microscopic study of 25 EM blocks of 18cases revealed seven AKs.

Linch CA, Prahlow JA, conducted a study on 2001 , Post-mortem Microscopic Changes Observed at the Human Head Hair Proximal End, in Dallas County Institute of Forensic Sciences, in the 22 postmortem cases. Cases were selected where an approximate time of death was known or could be determined from reviewing medical investigator reports. Hair proximal ends were photographed with a Zeiss compound microscope and observed. And he concluded that postmortem hair proximal end morphologic changes appear to be relatively specific for the decomposition process, but the timing of their appearance is not helpful in determining post-mortem

Bardale RV, Tumram NK, Dixit PG, Deshmukh AY, conducted a study at the Department of Forensic Medicine, Government Medical College Hospital, Nagpur, from March to April 2010. The study consists of 30 human cadavers comprising 24 male and 6 female, and their age ranged from 15 to 64 years. All samples were observed under light microscopy. Tissues were fixed in 10% formalin, embedded in paraffin wax, and stained with hematoxylin-eosin (H and E) and Periodic acid-Schiff (alcian-PAS). The skin samples were observed for the following: epidermis, dermis, sweat glands, sebaceous glands, and hair follicles. And they observed the following