Phytoplankton Lab Report

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Shea Gilkinson Humboldt State University, 1 Harpst Street, Arcata, CA 95521

Abstract Phytoplankton may be small, but they are the critical for the food web of the ocean. Because they are one of the primary producers it is important to understand how they absorb and retain energy for others to use it. Different conditions such as light and nutrients yield different outcomes. Using a fluorometer, the effects of light and nutrients absorption was measured based on growth rate and abundance of cultured phytoplankton Tetraselmis suecica. The results were in the form of p-values and converted to growth coefficients using different tests such as the 2 Sample T-Test and Mann-Whitney Test. Based on p-values and growth coefficients, it was then determined which sets of data were significant different(high nutrient vs. low nutrient & high light vs. low light). This showed that light was the primary factor for growth rate and nutrient were the primary factor for abundance.

Introduction There are many people that don’t think of phytoplankton first when hearing the word ocean. Instead many would think about larger …show more content…

The black vertical lines indicate the range of outliers in the data. The blue boxes represent the normal distribution of data within each sample. The black horizontal line included in the blue boxes represents the average for each sample. Growth rates were calculated using the equation µ = (1/t)*ln(N/No). “Here “µ” is the specific growth rate (in units of t-1) and “t” is the duration of the time over which you were measuring growth. For our purposes, “No” is the concentration of chlorophyll at the beginning of the experiment and “N” is the concentration of chlorophyll after time t has elapsed. Although “N” and “No” usually refer to actual numbers of cells, we will be using changes in bulk phytoplankton chlorophyll-a as a proxy for cell number.”(OCN 310

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