I used the article titled, “The Reaction of Oxy Hemoglobin with Nitrite: Mechanism, Antioxidant-Modulated Effect, and Implications for Blood Substitute Evaluation” from the journal Molecules. Hemoglobin is liable to participating in harmful reactions with other oxidized substances and thus creates unstable products. In this experiment they were trying to see if they can reduce the number of free radicals being created during certain steps of the process to produce blood substitutes. They measured the amount of oxy hemoglobin then measured how much reacted with the nitrate depending on what other substance it was paired with to test their hypothesis. The amount of the oxy hemoglobin as well as the nitrate influences the reaction time and how
Table 6 shows the results of the biochemical tests. The isolate can obtain its energy by means of aerobic respiration but not fermentation. In the Oxidation-Fermentation test, a yellow color change was produced only under both aerobic conditions, indicating that the EI can oxidize glucose to produce acidic products. In addition to glucose, the EI can also utilize lactose and sucrose, and this deduction is based on the fact that the color of the test medium broth changed to yellow in all three Phenol Red Broth tests. These results are further supported by the results of the Triple Sugar Iron Agar test. Although the EI does perform fermentation of these three carbohydrates, it appears that this bacterium cannot perform mixed acid fermentation nor 2,3-butanediol fermentation due to the lack of color change in Methyl Red and Vogues-Proskauer
Objective: The objective of the experiment is to determine what factors cause a change in speed of a reaction. It is also to decide if the change is correlated with the balanced equation of the reaction and, therefore, predictable. To obtain a reaction, permanganate, MnO_4^(1-), must be reduced by oxalic acid, C_2 O_4 H_2. The balanced equation for the reaction is:
Around 1902, when luminol was first synthesized, scientists noticed that it exhibited a blue glow in the presence of other compounds. Later on, it was found that the luminol reaction occurred in the presence of blood. Its use in crime scenes was first implemented by German forensic scientist Walter Specht in 1937. When the luminol solution is sprayed, the iron present in hemoglobin in blood catalyzes the reaction to produce a blue glow. This is used because in certain situations, there may be too little blood present at a crime scene to be able to be seen. It can also help when a suspect had attempted to “clean up” the blood after committing the crime. In these cases, luminol can be used to see any evidence of blood that cannot be seen with the naked eye. The glow from the reaction only lasts for about thirty seconds and requires dark conditions to be able to see, but investigators are able to document it in
For this experiment, you will add the measured amount of the first sample to the measured amount of the second sample into its respectively labeled test tube then observe if a reaction occurs. In your Data Table, record the samples added to each test tube, describe the reaction observed, if any, and whether or not a chemical reaction took place.
BioPure Corporation, which was founded in 1984 by entrepreneurs Carl Rausch and David Judelson, is a privately owned biopharmaceutical firm specializing in the ultra purification of proteins for human and veterinary use. In 1998 Biopure pioneered the development of oxygen therapeutics using “Hemoglobin”, a new class of pharmaceuticals that are intravenously administered to deliver oxygen to the body's tissues. Biopure's two products, Hemopure for human use, and Oxyglobin for animal veterinary use, both represented a new Oxygen based treatment approach for managing patients' oxygen requirements in a broad range of potential medical applications. The factor distinguishing Biopure’s two products from other blood substitute products being developed by two possible rivals, Baxter International and Northfeild Laboratories, is that its hemoglobin based source is bovine rather than human and was derived from the blood cells of cattle. Both of Biopure’s blood substitute products were in the final stages of the approval process of the Food and Drug Administration (FDA) in 1998. Oxyglobin had just received the FDA’s approval for commercial release declaring it safe and effective for medical use. Hemopure was entering final Phase 3 clinical trials and was optimistically expected to see final FDA approval for release in 1999. The FDA approval of Oxyglobin and its possible subsequent release into the veterinary market caused concern over whether the early release of Hemoglobin would impinge BioPure’s ability to price Hemopure when the product finally received approval. Given that the two products were almost identical in properties and function, it was thought that the early release of Oxyglobin would create an unrealistic price expectation for Hemopure if released first.
To complete this lab several chemicals must be measured and transferred to test tubes. First 5.0 mL of 0.200 M Fe(NO3)3 must be diluted to a total volume of 50 mL in a flask. Next 0.0020 M SCN–. This solution is then added to 4 test tubes in 1 mm increments. Each test tube is then put in to
... to demonstrate that hemoglobin attaches to the VIVO2+ ion at two locations of comparable strengths, named β and γ. This study has also proven that the interaction of red blood cells cannot be ignored when the conveyance or the pharmacological properties of a V compound is taken into consideration. In general, this paper does well in supporting the information available concerning hemoglobin. This article boosts the information available, concerning the diseases, genetics and functions of hemoglobin proteins. The authors achieve this by getting down to the basic level via the examination of the crystallographic structures of hemoglobin. This research has demonstrated novel examples associated with hemoglobin, pertaining to its processes and its purpose of movement. This study has immense implications for the grasp and the management of various diseases of hemoglobin
If the amount of either hydrogen peroxide or yeast is different in any of the sections in the experiment then the results
This form of testing is used for determining if an individual is inebriated or not. James and Nordby (2009) stated that an equilibrium exists between alcohol in the bloodstream and alcohol in the lung such that, on average, the concentration of blood alcohol is 2100 times greater than the concentration of breath alcohol (p. 63). Since this equilibrium exists, one can measure the breath alcohol and infer the corresponding alcohol concentration in the bloodstream (James & Nordby, 2009 p. 63). An advantage of this form of testing is that it can be conducted without drawing a blood sample and testing it. This allows personnel such as police officers to test individuals on the spot versus having to take someone in and draw a blood sample and wait for results to come
Observations: There was a fizz that occurred in the test tube which means a gas was produced. Also the mixture became warmer, and when putting the lit piece of wood in the tube, the flame went out immediately making a noise, which means that there was no oxygen
He tested it by using it on different subjects, later then realizing that he should mainly focus the transfusion apparatus on women with heavy bleeding because of the results due to hard labor, also called uterine hemorrhaging. That same year Blundell would publish a paper stating his work“Experiments on the Transfusion of Blood by the Syringe”. This ingenious paper discusses his patient's experiences with a full term blood transfusion using a syringe on both Animals and Humans.”He addressed the benefits of rapid execution to prevent coagulation, the importance of avoiding air intake in the veins and the incompatibility of heterologous donors”(Adams)The importance of avoiding air intake in the veins had great significance due to the causing of air bubbles that formed in the veins, called an air embolism now, which could attack by stroke, heart attack, or respiratory
The purpose of the lab was to show the effect of temperature on the rate of
However, in order to measure the rates of reaction, sodium thiosulphate and starch are added. Sodium thiosulphate is added to react with a certain amount of iodine as it is made. Without the thiosulphate, the solution would turn blue/black immediately, due to the iodine and starch. The thiosulphate ions allow the rate of reaction to be determined by delaying the reaction so that it is practical to measure the time it takes for the iodine to react with the thiosulphate. After the all the thiosulphate has reacted with the iodine, the free iodine displays a dark blue/black colour with the starch. If t is the time for the blue/black colour to appear, then 1/t is a measure of the initial rate.
Ties and hair should be tucked out of the way, so they do not make contact with any of the chemicals. It would also be preferable to wear a scientific apron, however this is not essential. We should also try our best not to spill any chemicals, and we must not eat or drink in the lab while dealing with these harmful chemicals, as they can get on our hands. Fair Test To make this experiment a fair test, we need to make sure we do a number of things. In this experiment we are trying to find the rate of reaction using concentration as a factor, so there are a number of things we need to make sure we do to keep it a fair test.
There is also the potential of human error within this experiment for example finding the meniscus is important to get an accurate amount using the graduated pipettes and burettes. There is a possibility that at one point in the experiment a chemical was measured inaccurately affecting the results. To resolve this, the experiment should have been repeated three times.