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How does solute concentration affect osmosis
The effect of solute concentration on the rate of osmosis
Ia introduction on osmosis
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INTRODUCTION
The purpose of this experiment is to show the effect of different concentration of solutions and the amount of osmotic activity between the solutions and three 3 naked eggs. A naked egg has a selectively permeable membrane. Osmosis is the crossing of solvent molecules from a region of higher concentration to a region of lower concentration through a selectively permeable membrane. In other words, a selectively permeable membrane means the control of substances or molecules passing in and out of the membrane by the cell membrane. A difference in solute concentration through a cell membrane will allow water flow by osmosis into the area with higher concentration of trapped molecules or substances. Concentrations with greater non permeable solute particles like protein in a solution, the
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The osmotic pressure of a hypotonic solution is lower than body fluids or other solutions; it swells by gaining water through osmosis. In hypertonic solutions, the osmotic pressure is higher than body fluids or other solutions; it loses water through osmosis and shrinks.
HYPOTHESIS
My hypothesis or guess is that the eggs will swell or increase in weight by showing that osmosis will occur by the different concentration of the solution so that a balance or osmotic equilibrium is reached.
MATERIALS USED
3 naked eggs
3 glass jars with lids
White vinegar
Karo (Corn syrup)
Water
Lemon juice
A ruler for measurement
A tiny rope/string
A flat plate to place the eggs on
A spoon to scoop the eggs from the jars
A camera to take pictures
A pen and a paper to note/record measurements of eggs
METHOD
Day 1 – Dissolving the shells of the eggs to make them naked
Carefully put the eggs one after the other in the three empty glass jars
Pour the white vinegar into the jars containing the eggs enough to cover the eggs
Cover the glass jars with the lids and place the jars in the fridge for 36 – 48hrs
DAY 2 – Jars in eggs still in the
Each subsequent trial will use one gram more. 2.Put baking soda into reaction vessel. 3.Measure 40 mL vinegar. 4.Completely fill 1000 mL graduated cylinder with water.
This process can also be defined as osmosis, which is the diffusion of water molecules across a membrane. (Unknown, 2) When we first got the egg it would be an isotonic solution meaning that it had equal tension (Unknown,1) which would mean that the same amount was inside the cell as outside of it. The purpose of the experiment was to learn about diffusion, concentration gradient, passive transport, equilibrium, osmosis, tonicity, hypertonic solutions, hypotonic solutions, isotonic solutions, and osmoregulation. If I put the egg in corn syrup, then the egg will deform because it has been placed in a hypertonic solution.
Diffusion and osmosis are necessary for the efficient transport of substances in and out of living cells. Diffusion is the most common and effective transportation process between cells and their surroundings, the movement of a substance along a concentration gradient from high to low, allowing essential nutrients and compounds to be transported without expending energy. Osmosis is a special kind of diffusion, specific to water. In order to observe diffusion and osmosis in real and artificial cells, a series of experiments was put together to observe how the surface area to volume ratio effects the rates of diffusion by using agar in different shapes with different ratios, next the rate of diffusion due to tonicity was observed using different solutions with different tonicities. And lastly live plant cells were submerged in different solutions with varying water potentials to observe how was potential effects the rate of osmosis and diffusion. It was concluded that the larger surface area to volume ratio, the faster rate of diffusion, the hypertonic solutions caused water to leave a cell and the hypotonic solutions allowed water to enter a cell, and that water potential will move from high to low in an attempt to maintain equilibrium.
Investigating the Concentration of Sucrose in Cells of Carrots and Potatoes Plan Introduction Osmosis is defined as 'the net movement of water molecules from a region of high water concentration to a region of lower water concentration through a partially permeable membrane'. In my experiment the process of osmosis will be due to the concentration of sucrose inside the cell compared to outside. Therefore if there is a higher concentration of sucrose molecules outside the cell then the net movement of water molecules will be out of the cell and similarly if there is a higher concentration of sucrose molecules inside the cell then the net movement of water molecules will be into the cell. This movement of water molecules causes the cells to become either turgid, which is when the cells are filled with water, or flaccid, which is when the cells are 'limp' with little water.
The water concentration is now even on the inside and out. This process is called osmosis. Part B: Aim: To investigate the action of a differentially permeable membrane. Method: See attached.
When the eggs are dropped onto the pillow, the eggs will bounce a little and stay whole.
This occurs when special carrier proteins carry solutes dissolved in the water across the membrane by using active transport. When the concentration gradient can not allow travel from one side of the membrane to the other fast enough for the cell’s nutritional needs, then facilitated diffusion is used. The transport protein is specialized for the solute it is carrying, just as enzymes are specialized for their substrate. The transport protein can be
Osmosis in Potato Tubes Osmosis: Osmosis is the movement of water molecules through a semi-permeable membrane from a high concentration to a low concentration. Diagram: [IMAGE] [IMAGE] Aim: To see the effects of different concentration of sugar solution on Osmosis in potato tubes. Key factor: In the investigation we change the sugar solution from: 0%-10%-20%-30%-40%-50% this is the independent variable; the dependant variable is the change in mass. Prediction: I predict that all the potato tubes in pure water or low concentration sugar solution will swell because water enters their cells by osmosis.
In osmosis, the proteins only on the surface are called peripheral proteins, which form carbohydrate chains whose purpose is used like antennae for communication. Embedded in the peripheral proteins are integral proteins that can either be solid or have a pore called channel proteins. -11.07% Discussion The chart shows the percent sucrose solution that resulted in the greatest change in average egg mass was the 0% solution, while the 1.73% solution resulted in the least change. The data also suggest that the percent sucrose solution that is isotonic to an egg’s interior may be between -10% and -15%, since the average masses of the eggs did not change beyond 0.15% over a 30 minute time period. In fact, the control group displayed the least amount of change in mass (0.10%) over a 40 minute period of time.
Investigate the Osmosis of Potato Cells in Various Salt Solutions. Introduction I have been asked to investigate the effect of changing the concentration of a solution on the movement of water into and out of potato cells. I will be able to change the input of my experiment. The input variable is the concentration of the solution.
Osmosis is the facilitated diffusion of water across the cell membrane of a cell. The inside layer of the cell membrane is hydrophilic, meaning water cannot easily pass through the membrane. The cell membrane has to have aquaporins, which are water channel proteins, that move the water across the membrane. If there is a water and salt solution outside the cell, the salt can enter the cell by diffusion, but the cell membrane is not permeable to the water. Because there is more solute solution inside the cell, there is less water. The aquaporins move the water across the membrane until equilibrium is reached.
Investigating the Effect of Sugar Solution on the Weight and Size of Potato Cells Aim: To investigate whether the different concentration of sugar solution will affect the weight and size of the potato cells. General background information: Osmosis is defined as the movement of water or any other solution's molecules from an area in which they are highly concentrated to a region in which they are less concentrated. This movement must take place across a partially permeable membrane such as a cell wall, which lets smaller molecules (E.g. water) through but does not allow larger solute molecules to pass through. The molecules will continue to diffuse until the area in which the molecules are found to reach a state of equilibrium, meaning that the molecules are equally distributed throughout the cell, with no area having a higher or lower concentration than any other hence equal. Hypothesis/prediction: For this particular investigation I believe that the lower the concentration of the sugar solution in the test tube the mass of the potato will be greater and the longer the potato cells will be.
Osmosis is the passage of water molecules from a weaker solution to a stronger solution through a partially permeable membrane. A partially permeable membrane only allows small molecules to pass through, so the larger molecules remain in the solution they originated in. Solute molecule [IMAGE] [IMAGE] Water molecule [IMAGE] The water molecules move into the more concentrated solution. When water enters a plant cell it swells up. The water pushes against the cell wall and the cell eventually contains all that it can hold.
== = This experiment is based on the concept of Osmosis. Osmosis is the diffusion of water molecules from a region of high water concentration to a low water concentration through a semi permeable membrane (in this case, the cell potato cell membrane). The cell walls of the potato cells are semi permeable meaning that water molecules (which are small) can fit through but other bigger molecules such as glucose cannot pass through. The water molecules can flow both ways through the membrane, letting molecules both in and out.
== § Test tubes X 11 § 0.10 molar dm -3 Copper (II) Sulphate solution § distilled water § egg albumen from 3 eggs. § Syringe X 12 § colorimeter § tripod § 100ml beaker § Bunsen burner § test tube holder § safety glasses § gloves § test tube pen § test tube method = == = =