My Personal Experience in Research Projects

595 Words2 Pages
bulb-icon

Recommended: Defining research

Research Experience I have proactively engaged in research activity throughout my undergraduate program. I had my first research experience in Dr. B Anand’s lab, Indian Institute of Technology (IIT), Guwahati, during my second year. The aim of the project was to study the non-fluorescent beta-barrel structured proteins and engineer its amino acid sequence to make them into fluorescent ones. Our approach was mainly based upon the studies of Green Fluorescent Protein (GFP) from a jellyfish Aequorea victoria. Tools like Python programming language, Gromacs, Ribosome, PROSS, and Pymol were used to study the structural components of non-fluorescent proteins. I gained invaluable knowledge about protein engineering and developed deeper fascination for life sciences. Later in the fall break, I interned at Globion India Pvt.Ltd–Hyderabad, India where I worked on ‘Growth-curve studies of chick embryo-propagated Infectious Bronchitis Virus’ under the mentorship of Dr. Sophia. My work included cultivating primary cell culture usi...

Show More
Related

  • Ligation of EGFP into pET41a(+) vector transformed into E. coli cells

    3721 Words  | 8 Pages

    Enhanced green fluorescent protein (EGFP) was originally isolated from a bioluminescent jellyfish called Aequorea victoria. As suggested by the name, this protein fluoresces green when exposed to light in the ultraviolet range. The ultimate goal of the following experiment was to successfully create a pET41a(+)/EGFP recombinant plasmid that was transformed into live E. coli cells. The success of this transformation could be evaluated based on whether EGFP’s fluorescence properties were displayed by the colony in question. The protein’s fluorescence properties “triggered the widespread and growing use of GFP as a reporter for gene expression and protein localization in a broad variety of organisms” (Ormo, et. al., 1996). Although EGFP and GFP differ for a few amino acids that make EGFP’s fluorescence mildly stronger, the basic principle that such a protein allows for the evaluation of transformation success remains intact.

    Read More

  • Pglo Plasmid Lab Report

    1179 Words  | 3 Pages

    The green fluorescent protein (GFP) gene is a naturally occurring gene from a bioluminescent jellyfish. The gene allows for objects and animals to glow in the dark when activated by the presence of the sugar arabinose in the pGLO plasmid. The GFP gene is often used as a marker for gene expression and genetic transformation. The pGLO plasmid is a genetically engineered plasmid used as a vector in biotechnology to generate genetically modified organisms (GMO). M. Chalfie et al.

    Read More

  • Freeman Dyson Our Biotech Future Summary

    827 Words  | 2 Pages

    Many people feel that biology has become more advanced than physics. Biology has in fact become the new focus of the future as we tend to use it a lot in our daily lives. The study of Biotechnology is known as the branch of molecular biology that studies the use of microorganisms to perform specific industrial processes. This study shows that our lives can be transformed.

    Read More

  • The Process of Research: The Institutional Review Board

    1524 Words  | 4 Pages

    Introduction During the process of research, professionals collect data or identifiable private information through intervention or interaction. While this is a vital part of the scientific and medical fields, every precaution must be taken by researchers to protect the participants' rights. Ethics, outlined by the Belmont report; requirements, described by the Department of Health and Human Services (DHHS); and regulations, laid out by the Food and Drug Administration (FDA) are verified by an Institutional Review Board (IRB). This procedure ensures that all human rights are safeguarded during the entire research process. The Institutional Review Board The IRB is an administrative body which has been established to make sure research participants' rights are protected.

    Read More

  • Being An Outsider Research Paper

    198 Words  | 1 Pages

    An outsider is someone who doesn’t belong to a particular group, but are outsiders most likely to be a boy or a girl? Females are most likely to be an outsider because there is more pressure on them in society to look good and be attractive. Girls are judged on the way they look and how they act. Between a boy and a girl, a girl is most likely to be an outsider because girls are expected to look a certain way and act a certain way in society.

    Read More

  • Critique of Research Design

    603 Words  | 2 Pages

    The purpose of this critique is to analyze the design of a research study conducted by; Donna Kazemi, Maureen Levine, Jacek Dmochowski, Mary Nies, and Linman Sun called “Effects of Motivational Interviewing Intervention on Blackouts Amoung College Freshman”. It was accepted in January 21st, 2013 and was published in the Journal of Nursing Scholarship.

    Read More

  • What I Learned in the Academic Research Course

    918 Words  | 2 Pages

    Throughout this semester in the Introduction to Academic Research course, I learned a great deal about research. We covered a wide variety of topics that encompassed topics such as methods of field research, the unknowns when conducting research (the Black Box activity), the differences that exist among fields when using the word “research”, and many others.

    Read More

  • Why I Want to Become a Biologist

    553 Words  | 2 Pages

    To excel in the field of Biology is not merely my dream, but my passion. I have started on this path of never-ending discovery and I want to master this science. It would not be unjustifying to state that the world is a better place today because of the advances in biological sciences. It truly promises to be an ever-advancing profession on this planet where better cures are required for freshly determined diseases on a day-to-day basis. Gene Technology and Biotechnology are a boon to this world. Putting microorganisms to use in the formation of insu...

    Read More

  • Personal Narrative: My First Retail Job

    2199 Words  | 5 Pages

    My experience with working with a group is that the longer you work together, it feels like the people I work with becomes a family. Sometimes we argue; sometimes we pick each other up when things are tough, but we also work together when things need to be accomplished. Getting a new job is always an adventure; normally, I walk through the door on the first day with fresh eyes and a fresh mind. That is exactly how I entered my first day at CVS Pharmacy (later to be known as CVS Health). Because this was my first retail job, it already seemed better than my experiences with the food and beverage industry. For about four years, I had been in and out of the restaurant business and believed that there had to be something a little more relaxed. Obviously, what I was doing was not my calling, which is why I applied to CVS. It had opportunity for advancement and a relaxed atmosphere. A friends’ wife that works for this particular CVS and helped me get my foot in the door. Walking through the double automatic doors, I could see the cashier, who I would later know as Alyssa, smiling at a customer. My “new” manager approached me with a genuine smile. Just the positive atmosphere and environment set the tone of how it would be working here.

    Read More

  • Observations from a Blind School Classroom

    795 Words  | 2 Pages

    For my Field Experience 1 observed Mrs. Vicki Banks a certified teacher of the visually impaired at the South Carolina School for Blind on September 27 and 28, 2016 during sixth period from 1:00 to 1:50 and then I met Mrs. Banks on November 1, 2016 during sixth period to interview her and ask her a few questions I had from the observations. Mrs. Banks selected a student to perform the Functional Vision Assessment that is diagnosed with Optic Nerve Hypoplasia. The student is a twelfth-grade student at the school.

    Read More

  • Biomedical Science: Personal Statement

    675 Words  | 2 Pages

    Another area of 14 interest for me is molecular biology, this being the biosciences departments strong suit at 15 Sheffield Hallam means I can pursue my interest in this topic with techniques that have been 16 developed in research as the techniques found in research are integrated into the course. 17 Research techniques being integrated ensures that the work being carried out is up to date and 18 means the information being delivered on the course keeps up with the ever-changing world of 19 science and I am keen to expand my knowledge and learning with these research techniques. A 20 further topic in the second year that I am interested in is the biomedical investigative 21 techniques as this will explore the problem-solving nature of science, and learning the 22 different investigative techniques will be useful and enjoyable due to the challenge in 23 investigation which is a key part of science. 24

    Read More

  • Why I Want To Pursue A Biomedical Science Degree

    499 Words  | 1 Pages

    As an undergraduate biomedical scientist at the federal university of my state, one of my greatest desires is to achieve all my academic goals and graduate with excellence in the career that I have chosen to follow. Since the beginning, I always have conducted my studies aiming to reach a high quality pattern as a student, enrolled in all academic experience that university could offer, including extracurricular activities and internship opportunities. Now, as a senior student, all my principal goals remains the same but much more focused on pursuing an academic career and trace new ambitions for my professional life as a scientific researcher. Furthermore, I want to be a professional capable to perform great biomedical researches and contribute in an innovative way to scientific community, expecting that all efforts will have impact on other people’s life care one day.

    Read More

  • Research Philosophy

    745 Words  | 2 Pages

    Research philosophy, refers to the development of knowledge adopted by the researchers in their research (Saunders, Lewis and Thornhill, 2009). In other words, it is the theory that used to direct the researcher for conducting the procedure of research design, research strategy, questionnaire design and sampling (Malhotra, 2009). It is very important to have a clear understanding of the research philosophy so that we could examine the assumptions about the way we view the world, which are contained in the research philosophy we choose, knowing that whether they are appropriate or not (Saunders, Lewis and Thornhill, 2009). According to Saunders, Lewis and Thornhill (2009), three major ways of thinking about research philosophy are examined: ontology, epistemology and axiology. Each of them carries significant differences which will have an impact on the way we consider the research procedures. Ontology, “is concerned with nature of reality”, while epistemology “concerns what constitutes acceptable knowledge in a field of study and axiology “studies judgements about value” (Saunders, Lewis and Thornhill, 2009, p110, p112, p116). This study is intent on creating some “facts” from objective evaluations which are made by the subjects. Therefore, epistemology will be chosen for this study as the way of thinking about the research philosophy.

    Read More

  • Essay On Biotechnology

    984 Words  | 2 Pages

    The myriad mysteries of science can be unraveled by the emerging technologies including Biotechnology. Science has always been my interest and forte thus, the choice of Biotechnology as my academic option was the ideal decision. I had prepared for the highly competitive entrance exam AIET to get admission into the integrated Masters Degree in Biotechnology and Bioinformatics at Dr. D.Y. Patil University and secured 87th all over India rank and was proud to gain admission to this venerated university. The academic curriculum has introduced me to amazing subjects like ‘Microbiology’, ‘Molecular Biology’, ‘Biochemistry’, ‘Genetics’ and ‘Industrial Biotechnology’. Although many seminal biological events have been explained in theory during the past century, the technology to harness their potential for benefiting humankind has only been possible during the past few decades. This is testament to the great improvements in biotechnologies and I am glad to be a part of this grand scientific experience.

    Read More

  • Statement Of A Masters Degree In Biotechnology

    814 Words  | 2 Pages

    Since school days the terms like cell, genes, DNA, protein intrigued me a lot and helped me in realizing the dream of pursuing a career in Biotechnology field that has been making many remarkable achievements. The passion towards biological sciences made me to take B.TECH in Biotechnology in Dr. M.G.R. University.

    Read More

More about My Personal Experience in Research Projects

Open Document