Mir2b Case Study

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If PGE4B is the target of miR-23b, we expect to see less amount of mRNA in the miR-treated cells than the scramble treated cells. We performed qPCR, then using the Ct values; we calculated the 2-∆∆Ct with the intention of comparing the level of RNA between treated cells and the control ones. We did the experiment twice and achieved two sets of data so that the result will not be products of randomness.
We expected all negative controls would show no amplification. However except for the negative control of the control in the second data set, all the other negative control showed amplification which means there were contaminations. The sources of unwanted materials could be from pipetting when the tip of the pipette touched the gloves. Contamination could also occur because the tubes were left open for so long before the samples were added. As a consequence, contaminants might get into the tubes. Fortunately, the Ct values of all the negative controls (except one was undetermined) were significantly higher than that of other’s. Additionally, the difference in ratio of expression between the targeted samples and the positive samples were high (66.56%). From that, we considered the effect of …show more content…

If a certain disease was caused by abnormally expression of PGE4B, we could, theoretically, inject the miR-23b and cure the disease. On the other hand, ifsomeone has a diseases caused by misregulation of PGE4B’s expression because of the unusual expression of miR-23b. In that case, we can change the expression of miR-23b itself to restore the imbalance in gene expression and cure the disease. Additionally, qPCR can only tell us that the gene is a target, but it does not give us information about whether the gene is directly or secondarily regulated by the miRNA [15]. This is another question that needs to be

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