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Basic concepts in microbiology
Introduction to the history of microbiology
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Biology is the study of living organisms divided into specialized fields that cover their morphology, physiology, anatomy, behavior, origin, and distribution. One of the most important fields within biology is microbiology, a field that details the function and behavior of microorganisms that remain invisible to the human eye. Using devices like electron microscopes scientist are able to identify, characterize, and record the morphologies and behaviors of various microorganisms. One of the most essential components of all organisms including microorganisms is their genetic information. With the development of microbiology over the past century, DNA has been identified as the macromolecule that carries genetic information. Some key experimentations …show more content…
The concept of microbiology emerged in the late 1600s when Antonie van Leeuwenhoek first observed bacteria and other microorganisms using a single lens microscope. The microscope manufactured and used by Leeuwenhoek served as a catalyst for the field of microbiology, exposing a whole world of microorganisms. With the utilization of tools like microscopes and methods like ink staining, scientist have been able to step into a seemingly invisible world and note the contribution of microorganisms to human life. These tools and methods have been essential to the discovery that DNA was the macromolecule charged with the transformational ability of genetic information in bacterial cells The first experiment to establish the …show more content…
The main goal of this experiment was to determine if DNA or proteins within bacteriophages were the hereditary material that entered a bacterial cell to direct the assembly of new viruses. This experiment followed in its predecessor’s footsteps and used tools developed in the field of microbiology for the observation of cellular behavior. The researchers split their main objective into two experiments. In one experiment researchers took a bacteriophage encoded with the element P, which is prominent in DNA but not proteins, and in the other experiment researchers used the bacteriophages(phages) holding the element S, which exists in proteins but not DNA. The mixtures were then allowed to infect bacteria. Researchers then placed the phage and bacteria mixture in a blender in order to remove the viruses from the bacterial cells. Researchers then placed the mixture in a centrifuge, forcing the bacterial cells to the bottom of the tube, separating the bacteria from the rest of the material. Hershel and Chase found that the supernatant fluid, liquid that housed the lighter part of the viruses that did not infect bacteria, held a majority of the P element initially introduced in the experiment. The S element that indicated the singular presence of DNA, was found to be transferred into the bacteria. The results from this experiment indicated that DNA
Many people feel that biology has become more advanced than physics. Biology has in fact become the new focus of the future as we tend to use it a lot in our daily lives. The study of Biotechnology is known as the branch of molecular biology that studies the use of microorganisms to perform specific industrial processes. This study shows that our lives can be transformed.
Bacteria play a large role in our health, the environment, and most aspects of life. They can be used in beneficial ways, such as decomposing wastes, enhancing fertilizer for crops, and breaking down of substances that our bodies cannot. However, many bacteria can also be very harmful by causing disease. Understanding how to identify bacteria has numerous applications and is incredibly important for anyone planning to enter the medical field or begin a career in research. Having the background knowledge of identifying an unknown bacteria may one day aid healthcare professionals diagnose their patient with a particular bacterial infection or help researchers determine various clinical, agricultural, and numerous other uses for bacteria.
During her presentation, Bonnie Bassler expends many different aspects of communication in a bacterial level and how successful communication is important to survival and efficient functioning. It is evident from her presentation that without a common language to communicate with, bacteria could not work as efficiently as they do, neither to immunize people nor create havoc in them. In her presentation, Bassler asserts the importance of the use of language in communication in bacteria, using rhetorical appeals – logical, ethical and emotional – and how it is consequently related to humans.
In 1951, Watson attended a meeting at the Cold Spring Harbor Laboratory in New York, where he heard the outcomes of the Hershey-Chase experiment. He was then convinced that DNA was the genetic material. He realized that if the structure of the DNA molecule was understood, it would be possible to suggest that ge...
E. coli are bacteria that can cause an infection in various parts of your body, including your intestines. E. coli bacteria normally live in the intestines of people and animals. Most types of E. coli do not cause infections, but some produce a poison (toxin) that can cause diarrhea. Depending on the toxin, this can cause mild or severe diarrhea.
All the bacteria on the petri dish took in the plasmid and are therefore alive. The gene did not glow because Arabinose was not present and they could not turn on the operon. LB/ Amp /Ara with pglo glowed because the arabinose turned on the operon that controlled glowing. The bacteria is now making arabinose, causing them to glow. Also, all the bacteria on the petri dish were resistant to Ampicillin. Some of the bacteria colonies that did not glow had metabolized, or used up, all the arabinose. Bacterial transformation has shown great promise and progress in medicine and agriculture. It helps with research of using Insulin to treat diabetics, creating, and inserting cell hosts. Botanists use bacterial transformation to experiment with plants to resist colder temperatures, playing with the ripening process, and their ability to form a resistance to pests. However, these are always contradicted with statements challenging the ethical and moral issues that have to be unravelled with much thought before the further use of bacterial transformation. This process has opened doors for a healthier future and new, easier ways to create life saving
Germ theory changed the scientific world; it also created a worldwide impact on people in many disciplines and in everyday life. Using the Natural science discipline of biology doctors and scientist used their research to determine that diseases and illnesses were caused from germs. Germ theory impacted how researchers interact with illnesses and gave them an avenue to look for cures and treatments. When realizing what caused illness ordinary people could learn how to prevent them and this changed how people lived their lives.
Discoveries in DNA, cell biology, evolution, and biotechnology have been among the major achievements in biology over the past 200 years, with accelerated discoveries and insight’s over the last 50 years. Consider the progress we have made in these areas of human knowledge. Present at least three of the discoveries you find to be the most important and describe their significance to society, health, and the culture of modern life. DNA (deoxyribonucleic acid) is a self-replicating molecule or material present in nearly all living organisms as the main constituent in chromosomes. It encodes the genetic instructions used in the development and functioning of all known living organisms and many viruses.
David Baltimore is a famous biotechnologist who has made a great impact on the field of biotechnology. But he is more than a biotechnician, Baltimore is an accomplished researcher, educator, administrator, and public advocate for science and engineering and is considered one of the world’s most influential biologists. (Broad Institute, n.d.). During his career he discovered the enzyme reverse transcriptase in the virus particles, this helped prove the process of RNA to DNA conversion. At the
The lab this week included deoxyribonucleic acid (DNA) isolation and gel electrophoresis. All living organisms depend on DNA in order to live. In fact, DNA is the “blueprint” passed from parents to their offspring. If the parents have any kind of genetic disorder it is highly possible it is passed on to their children. DNA is a complex structure made up of nucleotides. The reason I say complex is because each nucleotide contains a sugar, phosphate and one of four nitrogen bases. The nitrogen bases include adenine, thymine, guanine and cytosine. The base is what determines the genetic code for each molecule of DNA. I find it amazing you can see DNA that is grouped together with the naked eye.
Anatomy, microorganisms, and Leeuwenhoek’s microscope were all used to further advance the science of microbiology. The first simple microscope set the standard for others to observe microorganisms. Discovery of microorganisms paved a path for a new study of different life forms. Looking at the way the body functioned changed how the world had originally viewed it. Without Antoni van Leeuwenhoek’s discoveries, microbiology might not even exist today.
Virus, Infection, and Pathogens As you begin this course, it is important for you to develop an understanding of a few key terms. These key terms are virus, infection, pathogen, and blood borne pathogen. A thorough understanding of these terms will help you better understand the concepts in subsequent sections of the course.
Environmental pressures can change a species over time, based on Darwin’s theory of natural selection and “survival of the fittest”. If a pressure affects a large number of the same species, two outcomes are possible. The species will either become extinct, or more commonly, a small percentage of the original population will outlast the pressure (generally due to a mutation in its genes, making it resistant to a specific occurrence). The remainder of the species will reproduce the population, passing on their mutation to some of their offspring, until all organisms carry that mutation (often after several generations.
Afterwards genetic technology is implemented. Like using restriction enzymes to add to the DNA. These enzymes “cut” a specific pattern in our DNA molecules. These patterns will depend on the person since everyone has a unique set of markers. The second step after doing this would be using agarose gel, another genetic technological discovery, to separate long pieces of DNA from the short pieces that were “cut” up. This agarose gel was first put into an electrophoresis tray, then the DNA was added. What we will end up with is a small hole in the end of the tray. Then the electrophoresis tray is turned on.Electrophoresis is using electricity to move the DNA molecules. Since, DNA has a small negative charge so it move the pieces from the far end of the tray, negative, to the other end, positive. In logical terms, the smaller pieces of DNA will move faster, so the result will be DNA fragments in order from smallest to largest. Then since the DNA molecules are so thin and small an absorbent piece of paper called nylon membrane is placed on top of the gel to transfer it easier. Probes, which are radioactively labeled DNA, are added on top of the nylon. The probes only attach to the DNA that complements a particular code(kind of like RNA). Then older technology is used. X-ray film is put on top of that and the probes, which are radioactive and now in fewer locations of the DNA, will show up once the
I have been in awe of the natural biological phenomena involved in the complex processes of the life. My inquisitive nature prompted me to read about biological processes which helped me understand how small change could be responsible in creating a disease state. Fascination regarding the cause of these diseases encouraged me to pursue Microbiology. The years spent undertaking the undergraduate studies at The Y.C. College of Science, Shivaji University (lndia), was a pivotal phase of my life that significantly shaped my career. lt was during this period that I developed liking for Microbiology and Molecular biology and which made process of learning enjoyable and interesting. The inclination towards research in this