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Effect of temperature on enzymes
Temperature effects on enzyme function in biology
Effect of temperature on enzymes
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Investigation in to the factors that affects the rate of fermentation of Glucose
Aim:
To investigate the factors that affects the rate of fermentation of
Glucose.
Background knowledge.
ENZYMES
Fermentation is a form of an anaerobic respiration where by glucose is partially broken down so only a small amount of energy is released.
The yeast cells use the enzymes, ZYMASE to break down sugars unable to obtain. GLUCOSE CARBON DIOXIDE + ETHANOL(+ENERGY) C6 H12 O6 6CO2 + C2H5OH (210KJ)
Enzymes have several properties.
1) Specific actions-Zyamse in yeast works on glucose.
2) A small amount is required as the enzyme is used again and again as it is uncharged at the end therefore one of my variables could be varying the quantity of yeast.
3) Each enzyme has its own specific temperature at which it works best. This is the factor at which I am going to investigate zymase in yeast. There are some enzymes which work best in cold eg. Fridge. And others that can stand considerably high temperatures.
All enzymes are proteins as they are made by living cells. Therefore high temperatures cause protein to become DENATURED, destroyed, exaclty the same way as the cooked white of an egg cannot be changed back to its runny state. When proetin of an ezyme is denatured it no longer functions as a catalyst.
COLLISION THEORY
'Rate of reaction simply depends on how hard and often the reacting particles collide with each other.'
The basic idea is that particles have to collide with each other in order to react.
MORE COLLISIONS INCREASE THE RATE OF REACTION.
1) Temperature increases the number of collisions.
2) Concentration (or pressure) in creases the number of collisions.
3) Sizes of solid particles (or surface area) incr...
... middle of paper ...
...ature of 40-50°, therefore the molecules reacted faster and harder, collision theory, which starts to give off a gas, CO2. The glucose changes into a gas, Kinetic theory.
My graph of my results shows that the Carbon Dioxide produced did not react as fast as the enzyme which is working as a catalyst has either stopped or died. My prediction has been a success.
GLUCOSE CARBON DIOXIDE + ETHANOL(+ENERGY) C6 H12 O6 6CO2 + C2H5OH (210KJ)
Evaluation:
I really enjoyed doing this experiment, which is unusual! I did my research well. Using different types of materials, Internet, Library,
Revision books.
I think it would have been better if I had of done each temperature three times so that I could have found an average. It would have been abit more fairer and bit more accurate.
I also missed the first week so it was a little bit confusing at first but it turned out okay.
2. A test tube was then filled with 35ml of yeast and placed in the
The Holocaust could be best described as the widespread genocide of over eleven million Jews and other undesirables throughout Europe from 1933 to 1945. It all began when Adolf Hitler, Germany's newest leader, enforced the Nuremburg Race Laws. These laws discriminated against Jews and other undesirables and segregated them from the rest of the population. As things grew worse, Jews were forced to wear the Star of David on their clothing. The laws even stripped them of their citizenship.
The shape of the molecules is changing and so the enzyme molecules can no longer fit into the gaps in the substrate that they need to and therefore the enzymes have de – natured and can no longer function as they are supposed to and cannot do their job correctly. Changing the temperature: Five different temperatures could be investigated. Water baths were used to maintain a constant temperature. Water baths were set up at 40 degrees, 60 degrees and 80 degrees (Celsius). Room temperature investigations were also carried out (20 degrees).
or it may be optimum it depends on what is the best pH that the enzyme
Purpose: The purpose of this lab is to explore the different factors which effect enzyme activity and the rates of reaction, such as particle size and temperature.
How Amylase Concentration Affects the Rate of the Starch Concentration In this piece of coursework, I have to carry out an investigation to find out how amylase concentration affects the rate of starch. Enzymes are biological catalysts that speed up the chemical reaction that goes on inside living things. An enzyme acts on substrate and may do its job inside or outside the cells. However, the rate at which enzymes work are affected by the following factors/variables: Concentration:
The Effects of Concentration of Sugar on the Respiration Rate of Yeast Investigating the effect of concentration of sugar on the respiration rate of yeast We did an investigation to find how different concentrations of sugar effect the respiration rate of yeast and which type of concentration works best. Respiration is not breathing in and out; it is the breakdown of glucose to make energy using oxygen. Every living cell in every living organism uses respiration to make energy all the time. Plants respire (as well as photosynthesise) to release energy for growth, active uptake, etc…. They can also respire anaerobically (without oxygen) to produce ethanol and carbon dioxide as by-products.
According to Andrew Herman, “Each year, 14,000 die from drinking too much. 600,000 are victims of alcohol related physical assault and 17,000 are a result of drunken driving deaths, many being innocent bystanders” (470). These massive numbers bring about an important realization: alcohol is a huge issue in America today. Although the problem is evident in Americans of all ages, the biggest issue is present in young adults and teens. In fact, teens begin to feel the effects of alcohol twice as fast as adults and are more likely to participate in “binge-drinking” (Sullivan 473). The problem is evident, but the solution may be simple. Although opponents argue lowering the drinking age could make alcohol available to some teens not mature enough to handle it, lowering the drinking age actually teaches responsibility and safety in young adults, maintains consistency in age laws, and diminishes temptation.
Before development exploded in California, the state contained about 5 million acres of wetland habitat. Unfortunately, over the years California has been willing to part with 91 percent of its wetlands, Southern California having lost 95 percent. Los Angeles County has only one wetland remaining. This being the Ballona Wetlands located between Marina del Rey and the Westchester bluffs, it was once a major part of California’s natural wetland systems. Before development, Ballona wetlands natural habitat covered 2002 acres. This area, 800 to 1000 acres, referred to as Playa Vista by its owners is made up of salt marsh freshwater marsh and part upland and dune areas. It is considered Los Angeles’ largest ‘wetland ecosystem’. Although degraded over the years it still functions as a normal natural wetland - providing vital services. A major function it provides is that it filters out toxic wastes and pollutants from stormwater runoff before they reach the Santa Monica Bay. It is "a coastal plain, and acts as a flood plain for the surrounding area" (FOBW Information folder 1998: Fact Sheet). Ballona is an integral part of the Pacific flyaway, providing vital feeding and nesting habitat for over 185 species of birds, including some on the federal endangered species list. The southwestern willow flycatcher, an endangered species, was just recently seen by developers in an area cleared for construction. Ballona is no doubt a welcomed refuge to all species, both flora and fauna, from the industrial landscape of Los Angeles. The Friends of Ballona Wetlands have said it is "a spawning ground for commercial and sport fish, and a vital source of nutrients for the entire coastal marine environment" (FOBW Information folder 1998: 20 years of C...
This paper introduces the environmental concerns of the loss of coastal wetlands. The paper will discuss the significance of wetlands and the devastation that is occurring because of human activity. Wetlands are an essential element of our environment both ecological and societal; conservation will be essential for the preservation of these precious ecosystems.
It has been estimated that nearly half of the total areas of wetlands on Earth have been lost. The remaining wetlands still play important roles in their ecosystems and to the benefit of humans. The wetlands that remain occupy about 9% of Earth’s surface (land), but that 9% contribute largely to the planet, despite how small they seem to be. They support large amounts of biodiversity, improve and maintain water quality, and help prevent floods, meaning that if they are lost, things can go back fast.
As an American, when you turn eighteen years old, you’re legally allowed to enlist in the military, get married, buy tobacco, and have the right to vote. With having these responsibilities under your belt, many would assume the age to drink alcohol would be 18, but in America, you are not allowed to legally drink alcohol until you are 21. As many people turn 18 and enter adulthood, they should be able to make their own decisions about alcohol consumption. It should not be up to the government to dictate whether what age is appropriate to drink legally at, or let alone tell an adult what he or she can/cannot consume. From college parties, painful break-ups, the stress of
The pH of the solution would alter the rate of the reaction if it was
The biggest challenge investigators face and who is involved with high tech crime is the fast-paced constant evolving nature of technology. When companies come out with new devices or new versions of old devices which is almost all the time, and those who gather digital evidence must remain current to be able to locate and preserve all potential evidence. As technology evolves the capacities of these devices will rapidly increase while their form factor grows continually smaller. Investigators must preserve digital evidence to make sure it is suitable for presentation in court as well. Investigators must first never change a crime scene or alter evidence. It is their goal to document and preserve the scene exactly as it was when the crime occurred. Extreme caution and care is needed because the mere act of documenting or cataloging a crime scene means that investigators are interacting with the scene. The second concern is the physical fragility of the evidence. Care must be taken to keep items from getting wet, stepped on etc, this can also be applied to digital evidence. Investigators have been able to examine hard disk drives that have been through fires because the drives are usually air and water tight and impervious to temperatures into the thousands of degrees. The third issue is that digital evidence can be lo...
The Convention on Wetlands of International Importance Especially as Waterfowl defines wetlands as “areas of marsh, fen, peatland or water, whether natural or artificial, permanent or temporary, with water that is static or flowing, fresh, brackish or salt, incl...