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INTRODUCTION TO PLANT CELL CULTURING
TABLE OF CONTENTS
1. .INTRODUCTION TO BIOTECHNOLOGY Introduction and Definition o Historical Perspectives o Scope and Importance of Biotechnology o Commercial Potential
2. CELL GROWTH AND DEVELOPMENT
a) Cell division
b) Cell cycle
c) Animal and plant development
3. PLANT CELL CULTURE AND APPLICATIONS
a) Introduction
b) Cell- and Tissue-Culture Techniques
c) Applications of Cell and Tissue Culture
d) Gene-Transfer Methods in Plants
e) Transgenic Plants with Beneficial Traits
f) Diagnostics in Agriculture and Molecular Breeding
g) Bioethics in Plant Genetic Engineering
PREFACES
B iotechnology as a fast developing technology as well as a science has already shown its impact on different aspects of day-to-day human life such as public health, pharmaceuticals, food and agriculture, industry, bioenergetics and information technology. Now it is very clear that biotechnology will be a key technology for the 21st century and the science of the future. It has the potential to ensure food security, dramatically reduce hunger and malnutrition, and reduce rural poverty, particularly in developing countries. Considering its commercial potential and its possible impact on the economy, the
So what makes modern biotechnology modern? It is not modern in the sense of using various living organisms, but in the techniques for doing so. The introduction of a large number of new techniques has changed the face of classical biotechnology forever. These modern techniques, applied mainly to cells and molecules, make it possible to take advantage of the biological process in a very precise way. For example, genetic engineering has allowed us to transfer the property of a single gene from one organism to another. But before going into the details of biotechnology and the techniques that make it possible, let us first define
Modern biotechnology was born at the hands of American scientists Herb Boyer and Stain Cohen, when they developed “recombinant deoxyribonucleotide, (rDNA), [1] for medicinal purposes. Subsequently, biotechnologists started genetically engineering agricultural plants using this technology. A single gene responsible for a certain trait, from one organism (usually a bacterium) is selected altered and then ‘spliced” into the DNA of a plant to create an agricultural crop consisting of that...
Any organism that is genetically modified can be defined as a transgenic organism. The two main benefits of transgenic plants are that they can help increase yields without having to select against bad plants, and they can be resistant to toxins. In order to create a transgenic plant DNA ...
Pamela Ronald, a plant geneticist, presented a Ted Talk “The case for engineering our food”, Ronald points out that engineered genetics for our plants is not harmful, yet better for our environment and health. “Now, genetic modification is not new; virtually everything we eat has been genetically modified in some manner”(Ronald).
A Review on Impacts of Genetically Modified Food on Human Health. Open Nutraceuticals Journal, 4. This paper give the concept that genetic engineering provides a means to introduce genes into plants that are different in some respects from classical breeding. Gene transfer in animals will play a part in boundless possibilities of improving qualitative and quantitative traits. If the yield has negative effects of gene technology on animals, human, and environment should be considered.
The controversy of these issues stems from the immense potential in genetic sciences for both positive use and harmful misuse. Though the questions and fears of critics reflect the wisdom of caution, the potentially unlimited benefits mandate that we pursue these technologies.
This encyclopedia was extremely helpful. In not knowing all of the exact terms and basic knowledge of genetic engineering, it helped inform any reader of all this and more. The pages that had information on genetics and genetic engineering, had detailed definitions and descriptions for all the terms and ideas. Instead of focusing more towards the future of genetic engineering, it gave numerous facts about the technology and accomplishments of today. In addition to basic knowledge information, history, diagrams, and background information was provided. Including genetic testing, genes and their formation, and genetic background. The encyclopedia gave easy, organized, and accessible information to use.
The term GM foods or GMO (genetically-modified organisms) is most commonly used to refer to crop plants created for human or animal consumption using the latest molecular biology techniques (Whitman, 2000). These plants have been modified in the laboratory to offer desired traits such as increased resistance to herbicides or improved nutritional content. Also, genetic engineering techniques have been applied to create plants with the exact desired trait very rapidly and accurately. For example, this is done by the geneticist isolating the gene responsible for drought tolerance and inserts it into another plant. The new genetically-modified plant will now have gained drought tolerance as well.
We live in a world that is constantly changing and advancing thanks to technological advancements, especially in the field of molecular genetics. Today, we are discovering and implementing new ways to overcome the ill-fated symptoms developed as a result from poor health or accidents. We are also making advancements in the field of agriculture thanks to molecular genetics. As we all know, food is an essential entity in our lives and is abundant as well as relatively easy to obtain here in the United States. However, as good as it may sound, this is not necessarily true for developing countries. Many people in developing countries receive very little food, if any, due to its scarcity. It is estimated that in Asia alone, close to 800 million people go to bed hungry every night due to food shortage. This problem can be alleviated by turning to the production of genetically modified organisms (a.k.a. GMOs).
The concepts of human enhancement and biotechnology are fairly new terms in the world of ethics and medicine. These words, although far from being unfamiliar, are not often heard in the medical field except in special cases. However, in the past few years, the research and use of biotechnology is on the rise and becoming more prevalent under certain situations. This week’s reading focuses on the issues of biotechnology in a historical and modern context, yet also addresses the pros and cons of such developments.
For the biotechnology industry, the future is now. Biotech companies are producing new and improved drugs, mapping the genome, and creating artificial organs and body parts. The advent of these new products will increase the quality of life for those who have access to them. Advancements in the biotechnology field have received a lot attention by the press and publications. They have given the impression that it is almost imperative to learn about this fairly new field of study.
Any technological application that uses biological systems, living organisms, or derivatives thereof, to make or modify products or processes for specific use to benefit the lives of humans or other organisms, in bettering their lives. (Essays, UK. (November 2013). Can Genetic Engineering Be Regarded As Biotechnology Biology?. April 2014, http://www.ukessays.com/essays/biology/can-genetic-engineering-be-regarded-as-biotechnology-biology-essay.php?cref=1)
The 'Standard' of the 'Standard'. Agricultural Biotechnology:Using Genetic Engineering to produce super foods. Retrieved March 9, 2011, from http://suite101.com/content/agricultural-biotechnology.html. ILEIA editorial team (2001, December). Genetic engineering: not the only option [Electronic version].
Keener, K., Hoban, T. and Balasubramanian, R. 2014. Biotechnology and its applications. [online] Available at: http://www.ces.ncsu.edu/depts/foodsci/ext/pubs/bioapp.html [Accessed: 11 Apr 2014].
The myriad mysteries of science can be unraveled by the emerging technologies including Biotechnology. Science has always been my interest and forte thus, the choice of Biotechnology as my academic option was the ideal decision. I had prepared for the highly competitive entrance exam AIET to get admission into the integrated Masters Degree in Biotechnology and Bioinformatics at Dr. D.Y. Patil University and secured 87th all over India rank and was proud to gain admission to this venerated university. The academic curriculum has introduced me to amazing subjects like ‘Microbiology’, ‘Molecular Biology’, ‘Biochemistry’, ‘Genetics’ and ‘Industrial Biotechnology’. Although many seminal biological events have been explained in theory during the past century, the technology to harness their potential for benefiting humankind has only been possible during the past few decades. This is testament to the great improvements in biotechnologies and I am glad to be a part of this grand scientific experience.
New opportunities and challenges of human health are presented by the application of biotechnology to food. Biotechnology helps improve the quality and nutrition of foods consumed by people,this helps their health. Foods produced through biotechnology has been around for more than 15 years but people are still debating about how safe the food is. Biotechnology still have to plays its role by increasing agricultural productivity,especially in light of climate change. I believe that biotechnology is still going to help better th nutritional value of food. It can also assist farmers to increase their productivity so that they can feed their families and many people across the globe. The whole world should be using the food biotechnology because it has the ability to change the world,it can be to end world hunger .