Hand Washing And Disinfection Lab Report

I. Introduction

This report presents the analysis of an experiment conducted in order to investigate two critical methods of controlling the transfer of microbes, which are hand washing and disinfection. People live in the presence of an innumerable amount of microbes every day, and they can be found anywhere and anyplace, from everyday objects like doorknobs to the surfaces of human bodies. When working in a health care facility, it is crucial to prevent the contamination of sterile environments, which can otherwise lead to the spread of microbes. Contamination is what ultimately results in the spread of unwanted disease and infection in patients, leading to life- threatening situations. The two most common ways to prevent the transmission

Keep the water running as you dry your hands with a disposable towel.

f. Use the towel to turn off the faucet.

5. Open the plate and roll the same three fingers of your left hand directly onto the “after hand washing” portion of the plate. Put the lid back on.

6. Submit the plate for incubation at 37 degrees Celsius.

Test of alcohol- based hand rub-

1. Take a plate, while it is still closed, use a sharpie to divide the plate in half by drawing a line down the middle of bottom of the plate.

2. Label bottom of the plate “before hand washing” and “after hand- washing.” Also include name and date.

3. Open the plate and roll three fingers of your left hand directly onto the “before” portion of the plate. Put the lid back on.

4. Treat your hands with an alcohol- based hand gel. Use the technique recommended by the Centers for Disease Prevention and Control (CDC):

a. Apply product to the palm of one hand. Use amount as specified on the instruction label.

b. Rub hands together, covering all surfaces of hands and fingers, until hands are dry.

5. Open the plate and roll the same three fingers of your left hand directly onto the “after” portion of the plate. Put the lid back on.

6. Submit the plate for incubation at 37 degrees Celsius.

IV.

There could have been mistakes while following the hand washing procedure, such as not paying close attention to the details in the lab manual, using the incorrect amount of soap, not removing all of the soap, etc. There could have also been mistakes with hand sanitizing, including a failure to spread the gel throughout the hands properly, not using enough gel, forgetting to wait until the gel dried before touching other materials, etc. There also could have been accidental contamination of the Petri dishes themselves. This is the most likely reason why there was such a wide range in colony numbers after both hand washing and hand