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Giardia lamblia flashcard
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Giardia lamblia is a flagellated enteric protozoan parasite that is found in soil, food, and water (“Giardia lamblia” sec 1). This microorganism tends to live in the small intestines of humans. G. lamblia has two separate morphological stages. The first morphological stage is the trophozoite stage. In the trophozoite stage, this microorganism is shaped like a flattened pear. During this stage, the microbe is about ten to twelve micrometers long and about five to seven micrometers wide. Bilaterally the microbe is symmetrical and has two separate nuclei present. On the anterior side of the organism, there is a large sucking disk. This disk is the mechanism by which the parasite attaches to the mucosa of the host (“Morphology” par 1). The second morphological stage of G. lamblia is the cyst form. The cyst is generally egg-shaped and measures eight to fourteen micrometers in length. The width of the cyst is between seven and ten micrometers. When in this form, the original organelle is duplicated. Instead of just having the two nuclei, the microbe now exhibits four nuclei, four median ...
The unknown bacterium that was handed out by the professor labeled “E19” was an irregular and raised shaped bacteria with a smooth texture and it had a white creamy color. The slant growth pattern was filiform and there was a turbid growth in the broth. After all the tests were complete and the results were compared the unknown bacterium was defined as Shigella sonnei. The results that narrowed it down the most were the gram stain, the lactose fermentation test, the citrate utilization test and the indole test. The results for each of the tests performed are listed in Table 1.1 below.
Sordaria fimicola is a species of microscopic fungus that is an Ascomycete and are used to test for genetic variation in the lab setting (Sordaria fimicola: A Fungus used in Genetics, Volk). These organisms are what are called model organisms, or species that has been widely studied usually because it is easy to maintain and breed in a laboratory setting and has particular experimental advantages (Sordaria fimicola, Volk). S. fimicola, because it is in the Ascomycota phylum, have a distinguishing reproductive structure called the ascus, which is surrounded by the perithecium. This cylindrical sac-like structure houses 8 haploid spores; created through meiosis to produce 4 haploid spores and then mitosis to make 8 (Lab Manual, pg. 59-68). Based on the genotype they will vary in order and color. There are 3 different ratios that can arise from the 8 ascospores: 4:4, 2:2:2:2, and 2:4:2 (black/wild type and tan coloration). The 4:4 ratio suggests that no crossing over had occurred because there is no difference in order of the color parents that were mated. The two other ratios suggest genetic recombination, or crossing over, because of the
After 5 days of growth each slant was tested using the gram staining technique to confirm the complete isolation of the bacteria. Both isolations were completely successful. Then each sample of bacteria was subjected to a series of tests for identification.
The purpose of this laboratory is to learn about cultural, morphological, and biochemical characteristics that are used in identifying bacterial isolates. Besides identifying the unknown culture, students also gain an understanding of the process of identification and the techniques and theory behind the process. Experiments such as gram stain, negative stain, endospore and other important tests in identifying unknown bacteria are performed. Various chemical tests were done and the results were carefully determined to identify the unknown bacteria. First session of lab started of by the selection of an unknown bacterium then inoculations of 2 tryptic soy gar (TSA) slants, 1 nutrient broth (TSB), 1 nutrient gelatin deep, 1 motility
G. vaginalis cells are gram-negative to gram-variable. These cells are nonmotile, small, pleomorphic rods that do not have endospores, flagella, or typical capsules. In vaginal fluid smears, the Gram reaction may vary from positive to negative (Catlin, 1992). G. vaginalis is viewed in non-crowded areas in a Papanicolaou preparation. Their morphology and staining reactions are affected by the bacteria’s physiological state. Both coccobacilli and longer forms occur in 24 hour cultures of G. vaginalis on blood agar. Their average dimensions are 0.4 by 1.0 to 1.5µm. The cells do not elongate into filaments although they can be up to 2 to 3µm (Catlin, 1992).
Treponema pallidum is a Gram-negative bacterium that has a spiral shape (1). They are about 6 to 20um in length and 18-20um in diameter (1). Treponema pallidum can only survive within a host which makes them obligate internal parasites, meaning that outside a host this organism will die due to the absence of nutrients, exposure to oxygen and heat. Also since this organism can’t be grown on a culture medium, animal models are used to study T. pallidum (1). Treponema pallidum bacteria consist of an inner and outer membrane with a thin peptidoglycan cell wall lacking liposaccharides (1). They have a distinctive corkscrew motility with the aid of 6 endoflagellas (2). The specific classifications of Treponema pallidum are the following: Scientific name- Treponema pallidum, Common name- Syphilis. Treponema causes syphilis, yaws, and spinta. Borrelia causes Lyme disease and relapsing fever and Leptospira causing leptospirosis (5). What makes this microorganism special to the human population is that it causes infection to its host by entering the body through microscopic skin or mucous abrasions via direct personal contact with the lesions (sexual or kissing), blood transfusions, accidental bacterial inoculation, and transplacental passage (2). According to the CDC website there are 55,400 people diagnosed with Syphilis every year and 322 reported cases of congenital Syphilis in 2012 (6).
Ever wonder what kind of parasites are in your water, or how they can enter in to your body to make you very sick? Well it is most definite that no on want to get sick. The information found in this paper was collected over the past month, either by going to the library or by accessing information off the Internet at home, almost every night. The point that will be given to you is a little in information about the infectious disease called Schistosomiasis. The points the main points will be the causative agents, symptoms, hosts, methods of transmission and history of the disease. So lets see what Schistosomiasis is.
Necrotizing Fasciitis (flesh eating bacteria ) from an essay by Katrina Tram Duong, edited by S.N. Carson M.D.
This disease may be on the brink of being eradicated entirely but that doesn’t stop it from being one of the grossest things that anyone has ever seen. The parasite enters the body via unsafe drinking water. When people drink water infected with water fleas that have have been hosting the larvae of the parasite. There are no symptoms, to begin with, but about one year later, blisters on the feet or legs begin to develop and then the adult fully developed worm crawls out of the host body over a period of a few weeks. It’s said that the pain is intense and can incapacitate a person for weeks at a time. The pain can also continue for months after the infection. The parasite needs to infect a person at least once a year in order to continue. There were an estimated 3.5 million cases in 1986 but the disease has been greatly reduced over time and only 22 cases were reported in 2015. This disease will probably be the first parasitic disease to be completely eradicated in humans.
I. Heartworm Disease is a very serious disease that affects and kills thousands of dogs each year. II. I would like to share some important information about this disease, especially if you are an owner or plan to become an owner of a dog. III. I am the owner of a beautiful mix breed named Cocoa.
The domain for Klebsiella is Bacteria, it is in the Proteobacteria phylum, Gammaproteobacteria class, Enterobacteriales is the Order, the family is Enterobacteriacaea, the Genus is Klebsiella, and finally the species is Klebsiella pneumonia.
Researchers from the Animal Parasitic Laboratory and Agriculture Research Service of the United States Department of Agriculture concluded that the linage of Trichinella spiralis originated from Europe over several thousand years ago (Rosenthal, LaRosa, Zarlenga, Dumans, Chunyu, Mingyuan & Pozio, 2008). This is the approximate time when pigs were first domesticated in that region. This implies that the species Trichinella spiralis was introduced to the Americas from pigs. However, there is evidence that the early people of this world, hominid hunters, have consumed foodborne parasites by hunting wild game from millions of years before. Today, swine is governed on the ensuing transmission, and evolutionary diversification.
Bacterial cells, like plant cells, are surrounded by a cell wall. However, bacterial cell walls are made up of polysaccharide chains linked to amino acids, while plant cell walls are made up of cellulose, which contains no amino acids. Many bacteria secrete a slimy capsule around the outside of the cell wall. The capsule provides additional protection for the cell. Many of the bacteria that cause diseases in animals are surrounded by a capsule. The capsule prevents the white blood cells and antibodies from destroying the invading bacterium. Inside the capsule and the cell wall is the cell membrane. In aerobic bacteria, the reactions of cellular respiration take place on fingerlike infoldings of the cell membrane. Ribosomes are scattered throughout the cytoplasm, and the DNA is generally found in the center of the cell. Many bacilli and spirilla have flagella, which are used for locomotion in water. A few types of bacteria that lack flagella move by gliding on a surface. However, the mechanism of this gliding motion is unknown. Most bacteria are aerobic, they require free oxygen to carry on cellular respiration. Some bacteria, called facultatibe anaerobes can live in either the presence or absence of free oxygen. They obtain energy either by aerobic respiration when oxygen is present or by fermentation when oxygen is absent. Still other bacteria cannot live in the presence of oxygen. These are called obligate anaerobes. Such bacteria obtain energy only fermentation. Through fermentation, different groups of bacteria produce a wide variety of organic compounds. Besides ethyl alcohol and lactic acid, bacterial fermentation can produce acetic acid, acetone, butyl alcohol, glycol, butyric acid, propionic acid, and methane, the main component of natural gas. Most bacteria are heterotrophic bacteria are either saprophytes or parasites. Saprophytes feed on the remains of dead plants and animals, and ordinarily do not cause disease. They release digestive enzymes onto the organic matter. The enzymes breakdown the large food molecules into smaller molecules, which are absorbed by the bacterial cells. Parasites live on or in living organisms, and may cause disease. A few types of bacteria are Autotrophic, they can synthesize the organic nutrients they require from inorganic substances. Autotrophic bacteria are either photosynthetic or Chemosynthetic. The photosynthetic bacteria contain chlorophyll that are different from the plant chlorophyll. In bacterial photosynthesis, hydrogen is obtained by the splitting of compounds other than water.
Food borne illnesses are caused by consuming contaminated foods or beverages. There are many different disease-causing microbes, or pathogens. In addition, poisonous chemicals, or other harmful substances can cause food borne illnesses if they are present in food. More than two hundred and fifty different food borne illnesses have been described; almost all of these illnesses are infections. They are caused by a variety of bacteria, viruses, and parasites that can be food borne. (Center 1)
Perhaps the most readily noticeable characteristic of archaea and bacteria is their size. These domains are comprised of the smallest, self-sustaining forms of life on the planet. Bacteria and archaea are prokaryotic, thus lack a nuclear envelope and membrane-bound organelles. They also present with circular chromosomes, sometimes referred to as “ring-DNA”. Many archaea are known a...