Wait a second!
More handpicked essays just for you.
More handpicked essays just for you.
Experiments on testing organic molecules
Don’t take our word for it - see why 10 million students trust us with their essay needs.
Recommended: Experiments on testing organic molecules
To uncover organic compounds like carbohydrates, lipids, proteins and nucleic acid, by using tests like Benedict, Lugol, Biuret and Beta Carotene. Each test was used to determine the presents of different organic molecules in substances. The substances that were tested for in each unknown sample were sugars, starches, fats, and oils. Moreover, carbohydrates are divided into two categories, simple and complex sugars. Additionally, for nonreducing sugars, according to Stanley R. Benedict, the bond is broken only by high heat to make make the molecules have a free aldehydes (Benedict). As for Lipids, there are two categories saturated and unsaturated fats. One of the difference is that saturated fats are mostly solids and have no double bond (Campbell Biology 73). The Beta Carotene test works by dissolving in a lipid, thus giving it color to make it visible. Moreover, proteins are made out of amino acids that are linked by a polypeptide bond (Campbell Biology 75). The purpose of this experiment was to determine whether an unknown class sample or food sample had any carbohydrates, lipids, or proteins in it. The expected result of the lab was that some substances would be present while other would be absent.
Materials and Methods : The first
…show more content…
According to Sara Florine, “Starch, which is a complex carbohydrate composed of glucose molecules bound together, is a major component of [corn]” (Florine). Thus, the Lugol’s test came back “black” or positive. Furthermore, the results can be backed up by the nutrition label on a Chex cereal box, which has corn listed as one of the main ingredients in the cereal, which is a strach. (EWG’S Food Scores) Additionally, according to the Environmental Working Group, Chex cereal is also made with and processed with corn oil. (EWG’S Food Scores). It is a small amount of oil, but nonetheless its enough to make the Beta Carotene test come back positive for
The beet Lab experiment was tested to examine bio-membranes and the amount of betacyanin extracted from the beets. The betacyanin is a reddish color because it transmits wavelengths in red color and absorbs most other colors. The membrane is composed of a phospholipid bilayer with proteins embedded in it. The phospholipid bilayer forms a barrier that is impermeable to many substances like large hydrophilic molecules. The cells of beets are red and have large vacuoles that play a big role for the reddish pigment. This experiment aimed to answer the question, “How do cell membranes work?” The hypothesis we aim to test is: Cell membranes work as a fluid mosaic bilayer of phospholipids with many embedded proteins. We predicted that the 50% Acetone will break down the most betacyanin. Our hypothesis was proven wrong by our data collected. We could test our predictions by doing the experiment multiple times and compare the
When the solution remains the same, it means the solution is negative control and does not have sugar. The presence of starch can be detected by using the Lugol’s iodine solution. If the unknown A, B, C milk samples turn to a dark blue color during the Lugol’s test, then these samples are positive control and also contain starch in them. But if the solutions turn to yellowish brown, it means these solutions are negative control
The weight of the final product was 0.979 grams. A nucleophile is an atom or molecule that wants to donate a pair of electrons. An electrophile is an atom or molecule that wants to accept a pair of electrons. In this reaction, the carboxylic acid (m-Toluic acid), is converted into an acyl chlorosulfite intermediate. The chlorosulfite intermediate reacts with a HCL. This yields an acid chloride (m-Toluyl chloride). Then diethylamine reacts with the acid chloride and this yields N,N-Diethyl-m-Toluamide.
This experiment will determine the levels of Vitamin C present in each of the different fruit juices selected. The level of Vitamin C will be compared between each fruit juice to determine which one has the highest amount of Vitamin C. Several drops from one fruit juice will be mixed into an iodine solution then repeated with a different fruit juice. With every drop of fruit juice a chemical reaction occurs and the process continues until the solution becomes colorless. The reaction will vary in according to the amount of Vitamin C present in each one of the different fruit juices.
The purpose of this lab is to test an enzyme amylase from digestion system, which it has a big part in breaking down carbs into maltose, glucose and others. And our data by end of the lab could lead us to the specific conditions which are required for amylase in order to do its job perfectly.
Most of our data supported the hypothesis. The average DO concentration is 8.3 ppm, which is between the optimal range of 8 – 12 ppm, suggesting that the aquatic organisms have a rather healthy life with abundant amount of oxygen provided to them. The average temperature is 20, which is rather cool considering that the river was exposed to sunlight and the actual temperature we felt on that day. But it is also reasonable, since the river was flowing swiftly due to the windy weather, which reduces the amount of sunlight it absorbed. Also there were trees near the river that provided shade for the river, and we got the sample from the shaded area. The average alkalinity level is 120.8 ppm, which exceeds 80 ppm, indicating a well-buffered stream.
Many academic, scientific, and regulatory organizations are considering ways to establish the scientific basis to support and further validate claims for functional components or the foods containing them. Consumer interest in the relationship between diet and health has increased the demand for information about functional foods. More recently, the food industry, the agricultural community, and now consumers have shown a growing interest in the field of...
These bands were also different colors and pH levels. The bands were ordered from bottom to top, most basic to most acidic. This was partly due to the fact that the KOH was solid and landed at the bottom. The bands of color, especially the green and purple were the result of the denaturation and blending of the color. The color of anthocyanin was changed by the level of acid or alkali. These molecules can tell you the pH of any substance [2].The higher the proportion of H+ ions when comparing H+ and OH-, the lower the pH would be [3]. The bonds of the organic molecule are also generally weak [4]. This leads to the relative ease with which the molecule seperates into different colored
I believe my molecule could be a Ketone because of its very high boiling point and solubility. The boiling point is 145.5˚C. The molecule I have been given is very soluble in water. There are dispersion and Dipole-dipole forces between each Ketone molecule. Oxygen is more electronegative than carbon, so it tends to pull the electrons towards the oxygen.
When the Coris began to study carbohydrate metabolism, it was believed that glucose, a type of carbohydr...
The purpose of the unknown project was to present nursing microbiology students with an opportunity to demonstrate their ability to identify an unidentified bacterial sample by observing macroscopic and microscopic morphologies, utilizing biochemical tests, and performing a gram stain. Immediately after being given the unknown bacterial sample, which was labeled as #18, a gram stain was performed. The unknown tested as gram positive, which determined which biochemical tests were utilized. The tests and/or agars included two Tryptic Soy Agar plates, which
The entirety of this lab experiments was testing general basic understanding of lab procedures. It tested if I can follow the basic direction and with carefully deduction, concluded to what my unknown organism was in the end. I had no problem in the beginning and determining I was working with a Gram-positive organism. I felt my process of doing lab test grew faster over time because in the beginning, I was very slow to smear prep. However, this gradually improved over time and in addition, so did my use of the microscope.
Early on an August morning, Dr. Armstrong was tending to his lab as he did every morning. He organized his tools in a manner in which he thought suitable for his own needs, he swept the floors, as well as sanitizing his work station where he performed most of his research and experiments. The room was very gray with one single window that let little light into the room, there was no other means of light in the room except one long light above his examination table and a single desk lamp he kept in the corner of the room. There was a metal desk on one side of the room with a computer and many files on it, on the other side of the room separated by a glass wall, was a stainless steal table, and many instruments the doctor used. The doctor
Metabolism is defined as the chemical processes that take place within an organism, such as production of energy (Nature Education, 2014). One component of metabolism occurs through gas exchange. In animals, gas exchange occurs through a process called diffusion where materials move from levels of high concentration to low concentration (Freeman, 2013). It usually occurs in the respiratory system of animals, the lungs, where oxygen is inhaled and carbon dioxide is exhaled (Freeman, 2013). However, some animals may also exchange gases through other unique methods. For example, Rana pipiens, or leopard frogs, have vascularized skin that are extensively essential in their extremely wet habitat (Hoese and Abraham, 2014).
The purpose of the nutrient lab was to learn about the nutritional architecture of the foods most prevalent in the American diet. The hypothesis of the lab was if you add a specific reagent then you could test the presence of a certain type of nutrient. The hypothesis in the lab was tested by using five reagents and ten items of food.