Enzymes And Insane: How Does ADH Affect Alcohol

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Introduction: In this experiment it talked about enzymes and substrates. What are enzymes and substrates? Enzymes are proteins that act as catalysts and help complex reactions. Substrates are the substance that the enzymes act. The reaction studied in the exercise is the oxidation of an ethanol substrate, and an acetaldehyde product. The two electrons were removed from the ethanol to convert to the oxidized form of Nicotinamide Adenine Dinucleotide (NAD+) to form the reduced form, NADH. This reaction is called a dehydrogenation reaction. The reduced form of NADH represents a bond energy storage molecule. The enzyme alcohol dehydrogenase (ADH), used NAD+ as a cofactor is reduced in the reaction. The purpose of this experiment will be to determine …show more content…

Enzymes are proteins that act as a catalysts and help complex reactions. Enzymes work with substrates which bind to the active site. The active site is also where the enzymes take their exact place. ADH is an enzyme that uses NAD+ as a cofactor. How does ADH affect alcohol? ADH affect alcohol by breaking it apart before it is able to reach the kidneys, so the more ADH produced the quicker the alcohol particles are broken apart. A theory of the photometric assay is that light can be classified according to its wave length. This is why we used a spectrophotometer, we set the wavelength to a certain number and it measures the absorbance of the solution. Therefore the more light that passes through a specific substances has a higher …show more content…

Mainly because different enzymes have different pH, and temperatures that they act on with. Adding more substrate can causes the enzyme to increase in activity. In the experiment you have ethanol who worked best with the changes of ph and temperature, whereas methanol was not so successful with the changes. The four alcohols each had the same reaction group but each had different chain length. Ethanol has a 2 chain length, Propanol 3, Methanol 1, and Butanol 4. As you can see in graph 2, the preferred alcohol was ethanol and the least preferred was methanol. You see this because ethanol has the highest absorbance and methanol has the least. The size of the substrate can determine how quickly the enzymes is able to recognize

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