Enzyme Lab Report

My hypothesis was I think that if we change the temperature of the enzyme it will have an area where the enzyme works very well, but if we increase or decrease the temperature too much, it will slow the productivity. If we change the pH of the enzyme, there will be an area where it is extremely productive, but if it gets too acidic or basic the productivity will slow. If we change the concentration by increasing the amount of enzymes, it will be more productive. T think this because your are having more than 1 enzyme work on one task. I you decrease the amount of enzymes, the productivity will be slower because there are less enzymes working on that certain task. The data refutes my hypothesis because I found that the enzymes work best in one

condition, not an area as I first thought. However, my data supports my hypothesis because the mL produced went down as you decreased the concentration and made it more acidic and basic.
This experiment showed that the catalase was most productive when it was at 100% catalase. At 4 minutes, with 100% catalase, 83 mL of O2 was produced compared to 56 mL with 75% catalase. At the end of the 10 minutes, 100% catalase produced 102 mL of O2, while 50% catalase only produced 65 mL of O2. This shows that the more enzymes in the solution, the faster the reaction is. This is why if it was 0% catalase, the reaction wouldn’t occur. However, the fact that with 25% less catalase, it was still able to increase the speed of reaction shows homeostasis. At the end of 10 minutes, with 75% catalase 100 mL of O2 was produced, compared to 100% catalase which produced 102 mL of O2. This shows how important enzymes are to keeping homeostasis. Another thing this experiment showed is that the catalase works best a a pH level of 7. For example, at the end of 10 minutes when the pH level was 11-12 only 2 mL of O2 was produced, but when the pH level was 7 102 mL of O2. This because when the pH was increased or decreased to much, it became denatured and didn’t increase the reaction rate. While the amount of mL being produced with a pH of 7 was rapidly increasing, the amount of mL being produced with a pH of 2-3 was very slowly increasing.